FuncClass

GlycoEnzDB
Comprehensive glycoEnzyme database and dynamic pathway generation

Description: The GlycoEnzDB is a manually curated glycoEnzyme database, primarily focused on humans. It covers 390 enzymes across 28 pathway maps. Facilities are also available to create custom glycosylation reaction pathways using experimental data in SBML format and for pathway simulation
Contacts: Sriram Neelamegham (neel@buffalo.edu), Yusen Zhou (yusenzho@buffalo.edu) or Ted Groth (tgroth@buffalo.edu).

GlycoEnzDB
Create glycosylation pathway
Custom database generation
Instructions/Reference
 
Pathway Map
FKTN



General Information
Gene Symbol:   
FKTN
Protein name:   
Fukutin
Enzyme Class:   
CAZy Family:   
E.C. No:   
2.4.2.*
Organs:   
Reaction (SNFG):   
+
→
+ UDP
Reaction (IUBMB):   
No specified reaction.
About

• A series of reactions occur on the core M3 O-mannosyl trisaccharide of α-dystroglycan (DAG1), and these elongate the core structure.
• Among these, first, two phospho-ribitols are added to the trisaccharide core. The first phospho-ribitol is added to GalNAc by FKTN/fukutin. The second ribitol is added by Fukutin-related protein/FKRP. Together, this results in the ribose 5-phosphate tandem repeat of DAG1.
• This unit is then primed by TMEM5 () and B3GAT1 (formerly B4GAT1).
• The core M3 structure can then be further extended by repeating GlcAβ(1-3)Xylα(1-3) units. This polymerization bifunctional GTs, LARGE1 (formerly LARGE) and/or its paralog LARGE2 (formerly GYLTL1B) in the Golgi apparatus.

Related Pathway
KEGG ORTHOLOGY:   
K19872
KEGG Pathway:   
No Record 
Reactome:   
No Record
Related Database
Organism:   
Homo sapiens
Uniprot:   
O75072 
NCBI GeneID:   
2218
GlyMap GeneID:   
2218
DNA aRefSeq:   
NM_001079802.1 
Protein RefSeq:   
NP_001073270.1 
BRENDA:   
Kinetic info.
OMIM number:   
607440
GeneCards database:   
FKTN
Enzyme Specificity
Glycan Type:  
     O-linked (other)
Compartment:  
     Cis, Medial, Trans, TGN
Substrate:  
     GalNAc(b1-3)GlcNAc(b1-4)Man(a1-?)Ser|Thr
Product:  
     Rib()P(-CHAR)GalNAc(b1-3)GlcNAc(b1-4)Man(a1-?)Ser|Thr
Simulation Parameters
Mechaelis-Menten parameters (suggested)
VM:       (pmol/cell • hr)       KM:       (pM)
Relative conc of enzymes in compartment.(sum will be normalized to 1)
ER Cis- Medial- Trans- TGN










Expression Data in Tissue {ref. 5} Source from NCBI gene database
adrenal appendix bone marrow brain colon duodenum endometrium esophagus fat gall bladder heart kidney liver lung lymph node ovary pancreas placenta prostate salivary gland skin small intestine spleen stomach testis thyroid urinary bladder 4 2 0 RPKM
Expression Data in Cells {ref. 13} Source from Cancer Cell Line Encyclopedia database
Kidney Lung Breast Prostate Large intestine Ovary HL60 haematopoietic and lymphoid tissue Skin Central nervous system 786O A498 ACHN CAKI1 UO31 A549 EKVX HOP62 HOP92 NCIH226 NCIH23 NCIH322 NCIH460 NCIH522 SHP77 BT549 HS578T MCF7 MDAMB231 MDAMB468 T47D DU145 PC3 HCT116 HCT15 HT29 KM12 SW620 OVCAR4 OVCAR8 IGROV1 SKOV3 HL60 K562 RPMI8226 LOXIMVI MALME3M RPMI7951 SKMEL28 SKMEL5 UACC257 UACC62 SF268 SF295 SF539 SNB75 40 30 20 10 0 Transcripts per million (TPM)
Transcription factor {ref. 10}
No high degree of confidence data