FuncClass

GlycoEnzDB
Comprehensive glycoEnzyme database and dynamic pathway generation

Description: The GlycoEnzDB is a manually curated glycoEnzyme database, primarily focused on humans. It covers 390 enzymes across 28 pathway maps. Facilities are also available to create custom glycosylation reaction pathways using experimental data in SBML format and for pathway simulation
Contacts: Sriram Neelamegham (neel@buffalo.edu), Yusen Zhou (yusenzho@buffalo.edu) or Ted Groth (tgroth@buffalo.edu).

GlycoEnzDB
Create glycosylation pathway
Custom database generation
Instructions/Reference
 
Pathway Map
FUT1



General Information
Gene Symbol:   
FUT1
Protein name:   
Galactoside 2-alpha-L-fucosyltransferase 1
Enzyme Class:   
Glycosyltransferase
CAZy Family:   
GT11
E.C. No:   
2.4.1.344
Organs:   
Reaction (SNFG):   
+
→
+ GDP
+
→
+ GDP
+
→
+ GDP
Reaction (IUBMB):   
No specified reaction.
About

• FUT1 and FUT2 α2-fucosyltransferases are encoded by the H and Se genes, respectively. Sec1 is a related pseudogene that has no functon in humans.
• FUT1 and FUT2 transfer fucose in α(1,2) linkage onto the terminal galactose of lactosamine predominantly on type 1 (Galβ1,3GlcNAcβ1-R) and type 2 (Galβ1,4GlcNAcβ1-R) chains. However, these enzymes can also work on type 3 (Galβ1,3GalNAcα1-R), type 4 (Galβ1,3GalNAcβ1-R), type 5 (Galβ1,3Galβ1-R), or type 6 (Galβ1,4Glcβ1-R) disaccharide acceptors although less efficiently.
• Final products of α2-fucosyltransferases are: CD173 or monofucosylated H type 2 (Fucα1,2Galβ1,4GlcNAcβ1-R) & CD174 or difucosylated Ley (Fucα1,2Galβ1,4[Fucα1,3]GlcNAcβ1-R).
• FUT1 (H) is expressed predominantly in erythroid tissues and primary sensory neurons (cells originating in the ecto- and meso-derm).
• Tissue specific distribution of a(1-2)FUT activity in rodent models is quite different from humans.
• The fucosylated glycan products of FUT1 and FUT2 may serve as ligands in cell adhesion or as receptors for certain microorganisms and play a role in susceptibility to infection.

Related Pathway
KEGG ORTHOLOGY:   
K00718
KEGG Pathway:   
ko00601  ko00603  ko01100 
Reactome:   
R-HSA-9033807
Related Database
Organism:   
Homo sapiens
Uniprot:   
P19526 
NCBI GeneID:   
2523
GlyMap GeneID:   
2523
DNA aRefSeq:   
NM_000148.3 
Protein RefSeq:   
NP_000139.1 
BRENDA:   
Kinetic info.
OMIM number:   
211100
GeneCards database:   
FUT1
Enzyme Specificity
Glycan Type:  
     N-linked, O-linked (mucin type), GSL
Compartment:  
     Cis, Medial, Trans, TGN
Substrate:  
     Gal(b1-?)...Gal(b1-?) or Gal(b1-?)GalNAc(b1-?) or Gal(b1-?)GlcNAc(b1-?)
Product:  
     Fuc(a1-2)Gal(b1-?)...Gal(b1-?) or Fuc(a1-2)Gal(b1-?)GalNAc(b1-?) or Fuc(a1-2)Gal(b1-?)GlcNAc(b1-?)
Simulation Parameters
Mechaelis-Menten parameters (suggested)
VM:    3.75e-06    (pmol/cell • hr)       KM:    1.40e+09    (pM)
Relative conc of enzymes in compartment.(sum will be normalized to 1)
ER Cis- Medial- Trans- TGN










Expression Data in Tissue {ref. 5} Source from NCBI gene database
adrenal appendix bone marrow brain colon duodenum endometrium esophagus fat gall bladder heart kidney liver lung lymph node ovary pancreas placenta prostate salivary gland skin small intestine spleen stomach testis thyroid urinary bladder 4 2 0 RPKM
Expression Data in Cells {ref. 13} Source from Cancer Cell Line Encyclopedia database
Kidney Lung Breast Prostate Large intestine Ovary HL60 haematopoietic and lymphoid tissue Skin Central nervous system 786O A498 ACHN CAKI1 UO31 A549 EKVX HOP62 HOP92 NCIH226 NCIH23 NCIH322 NCIH460 NCIH522 SHP77 BT549 HS578T MCF7 MDAMB231 MDAMB468 T47D DU145 PC3 HCT116 HCT15 HT29 KM12 SW620 OVCAR4 OVCAR8 IGROV1 SKOV3 HL60 K562 RPMI8226 LOXIMVI MALME3M RPMI7951 SKMEL28 SKMEL5 UACC257 UACC62 SF268 SF295 SF539 SNB75 40 30 20 10 0 Transcripts per million (TPM)
Transcription factor {ref. 10}
No high degree of confidence data
miRNA-GlycoEnzyme Interactome {ref. 9}
miRNA Name Pos. Context Score Frac. Wt. Context Frac. Conserved Quality
hsa-miR-1538 1308-1315 -0.61 97.0 -0.61 98.0 noncon
hsa-miR-4745-3p 1308-1315 -0.581 97.0 -0.581 98.0 noncon
hsa-miR-512-3p 2165-2172 -0.571 99.0 -0.571 99.0 noncon
hsa-miR-4473 2166-2173 -0.531 98.0 -0.138 78.0 noncon
hsa-miR-340-3p 894-901 -0.472 96.0 -0.472 97.0 noncon
hsa-miR-6827-3p 894-901 -0.466 95.0 -0.466 97.0 noncon
hsa-miR-449c-3p 189-196 -0.424 98.0 -0.424 98.0 noncon
hsa-miR-423-5p 1499-1506 -0.415 97.0 -0.415 98.0 noncon
hsa-miR-6748-3p 499-506 -0.407 98.0 -0.407 98.0 noncon
hsa-miR-6754-3p 534-541 -0.399 98.0 -0.399 99.0 noncon
hsa-miR-3184-5p 1499-1506 -0.399 97.0 -0.399 97.0 noncon
hsa-miR-412-3p 534-541 -0.385 98.0 -0.385 98.0 noncon
hsa-miR-6865-3p 1993-2000 -0.361 97.0 -0.361 98.0 noncon
hsa-miR-4308 1603-1610 -0.354 98.0 -0.354 99.0 noncon
hsa-miR-5001-5p 257-264 -0.354 95.0 -0.354 96.0 noncon
hsa-miR-4763-5p 217-224 -0.353 92.0 -0.353 95.0 noncon
hsa-miR-5580-5p 40-47 -0.343 96.0 -0.343 97.0 noncon
hsa-miR-556-5p 1632-1639 -0.342 98.0 -0.342 99.0 noncon
hsa-miR-548au-3p 1640-1647 -0.341 96.0 -0.341 97.0 noncon
hsa-miR-132-5p 2125-2131 -0.338 91.0 -0.338 94.0 noncon
hsa-miR-4498 257-264 -0.336 95.0 -0.336 96.0 noncon
hsa-miR-1183 268-275 -0.327 98.0 -0.327 99.0 noncon
hsa-miR-4671-3p 2167-2173 -0.318 92.0 -0.087 63.0 noncon
hsa-miR-4755-3p 64-71 -0.316 96.0 -0.316 97.0 noncon
hsa-miR-6734-5p 1500-1507 -0.311 94.0 -0.311 95.0 noncon
hsa-miR-3657 1615-1621 -0.31 94.0 -0.31 96.0 noncon
hsa-miR-1255b-5p 2048-2055 -0.308 95.0 -0.308 96.0 noncon
hsa-miR-6735-3p 698-705 -0.305 97.0 -0.305 98.0 noncon
hsa-miR-1255a 2048-2055 -0.305 95.0 -0.305 96.0 noncon
hsa-miR-3151-5p 1491-1498 -0.302 96.0 -0.302 97.0 noncon
hsa-miR-3194-5p 213-220 -0.294 95.0 -0.294 96.0 noncon
hsa-miR-34c-5p 1567-1574 -0.291 90.0 -0.291 93.0 noncon
hsa-miR-449b-5p 1567-1574 -0.291 90.0 -0.291 93.0 noncon
hsa-miR-34a-5p 1567-1574 -0.289 90.0 -0.289 93.0 noncon
hsa-miR-7113-3p 2078-2084 -0.287 97.0 -0.287 98.0 noncon
hsa-miR-6862-3p 2130-2137 -0.284 94.0 -0.284 96.0 noncon
hsa-miR-4435 1939-1946 -0.284 95.0 -0.284 97.0 noncon
hsa-miR-3192-5p 1836-1843 -0.283 98.0 -0.283 98.0 noncon
hsa-miR-4772-5p 26-32 -0.277 93.0 -0.277 95.0 noncon
hsa-miR-550a-5p 693-700 -0.275 92.0 -0.275 94.0 noncon
hsa-miR-550a-3-5p 693-700 -0.275 92.0 -0.275 94.0 noncon
hsa-miR-4652-5p 404-410 -0.273 91.0 -0.273 94.0 noncon
hsa-miR-3166 1708-1715 -0.271 91.0 -0.271 94.0 noncon
hsa-miR-1324 171-178 -0.271 97.0 -0.271 98.0 noncon
hsa-miR-6784-3p 2130-2137 -0.271 94.0 -0.271 96.0 noncon
hsa-miR-1271-3p 693-700 -0.267 91.0 -0.267 94.0 noncon
hsa-miR-214-5p 1534-1540 -0.265 96.0 -0.265 97.0 noncon
hsa-miR-762 257-264 -0.26 94.0 -0.26 95.0 noncon
hsa-miR-4492 257-264 -0.26 95.0 -0.26 96.0 noncon
hsa-miR-4319 2077-2083 -0.259 93.0 -0.259 95.0 con
hsa-miR-6822-3p 37-44 -0.259 94.0 -0.259 96.0 noncon
hsa-miR-19a-5p 193-200 -0.256 97.0 -0.256 98.0 noncon
hsa-miR-4308 1611-1618 -0.255 96.0 -0.255 97.0 noncon
hsa-miR-6802-3p 1555-1561 -0.251 90.0 -0.251 93.0 noncon
hsa-miR-873-5p.1 93-100 -0.251 98.0 -0.251 99.0 noncon
hsa-miR-4723-3p 451-458 -0.25 94.0 -0.25 96.0 noncon