FuncClass

GlycoEnzDB
Comprehensive glycoEnzyme database and dynamic pathway generation

Description: The GlycoEnzDB is a manually curated glycoEnzyme database, primarily focused on humans. It covers 390 enzymes across 28 pathway maps. Facilities are also available to create custom glycosylation reaction pathways using experimental data in SBML format and for pathway simulation
Contacts: Sriram Neelamegham (neel@buffalo.edu), Yusen Zhou (yusenzho@buffalo.edu) or Ted Groth (tgroth@buffalo.edu).

GlycoEnzDB
Create glycosylation pathway
Custom database generation
Instructions/Reference
 
Pathway Map
FUT5



General Information
Gene Symbol:   
FUT5
Protein name:   
Alpha-(1,3)-fucosyltransferase 5
Enzyme Class:   
Glycosyltransferase
CAZy Family:   
GT10
E.C. No:   
2.4.1.65
Organs:   
Reaction (SNFG):   
+
→
+ GDP
+
→
+ GDP
Reaction (IUBMB):   
No specified reaction.
About

• The gene is only sparsely expressed and its physiological function remained largely unknown.
• Recombinant FUT5 can utilize both type 1 and type 2 LacNAc chains and exhibits α1,3/4 fucosyltransferase activity.
• FUT3/FUT5/FUT6 gene cluster is not found in rodents. These are only expressed in humans and higher primates.

Related Pathway
KEGG ORTHOLOGY:   
K07633
KEGG Pathway:   
ko00601  ko01100 
Reactome:   
R-HSA-9037629
Related Database
Organism:   
Homo sapiens
Uniprot:   
Q11128 
NCBI GeneID:   
2527
GlyMap GeneID:   
2527
DNA aRefSeq:   
NM_002034.2 
Protein RefSeq:   
NP_002025.2 
BRENDA:   
Kinetic info.
OMIM number:   
136835
GeneCards database:   
FUT5
Enzyme Specificity
Glycan Type:  
     N-linked, O-linked (mucin type), GSL
Compartment:  
     Cis, Medial, Trans, TGN
Substrate:  
     ...Gal(b1-4)GlcNAc(b1-?) or ...Gal(b1-3)GlcNAc(b1-?)
Product:  
     ...Gal(b1-4)[Fuc(a1-3)]GlcNAc(b1-?) or ...Gal(b1-3)[Fuc(a1-4)]GlcNAc(b1-?)
Constraints:  
     !GlcNAc(b1-6)[GlcNAc(b1-3)]Gal(b1-4)@GlcNAc(b1-?)
Simulation Parameters
Mechaelis-Menten parameters (suggested)
VM:    3.75e-06    (pmol/cell • hr)       KM:    1.40e+09    (pM)
Relative conc of enzymes in compartment.(sum will be normalized to 1)
ER Cis- Medial- Trans- TGN










Expression Data in Tissue {ref. 5} Source from NCBI gene database
adrenal appendix bone marrow brain colon duodenum endometrium esophagus fat gall bladder heart kidney liver lung lymph node ovary pancreas placenta prostate salivary gland skin small intestine spleen stomach testis thyroid urinary bladder 8 6 4 2 0 RPKM
Expression Data in Cells {ref. 13} Source from Cancer Cell Line Encyclopedia database
Kidney Lung Breast Prostate Large intestine Ovary HL60 haematopoietic and lymphoid tissue Skin Central nervous system 786O A498 ACHN CAKI1 UO31 A549 EKVX HOP62 HOP92 NCIH226 NCIH23 NCIH322 NCIH460 NCIH522 SHP77 BT549 HS578T MCF7 MDAMB231 MDAMB468 T47D DU145 PC3 HCT116 HCT15 HT29 KM12 SW620 OVCAR4 OVCAR8 IGROV1 SKOV3 HL60 K562 RPMI8226 LOXIMVI MALME3M RPMI7951 SKMEL28 SKMEL5 UACC257 UACC62 SF268 SF295 SF539 SNB75 2 0 Transcripts per million (TPM)
Transcription factor {ref. 10}
Transcriptional start site (TSS) 0 200 -2000 -1800 -1600 -1400 -1200 -1000 -800 -600 -400 -200
ENST00000588525 SP5 PPARG PPARG NR2F6 ZNF644 GATAD2A RARA RARA RXRB MIXL1 CEBPA SOX13 SOX13 HNF4G HNF4G REST JUND SP1 RXRA RXRA RXRA FOXA2 MIER3 TFAP2A ATF1 ATF3 CREB1 ZBTB26 MAX L3MBTL4 TFAP4 KLF5 SCRT1 FOXA1 MXD3 FOXA2 ZNF7 RCOR2 ZBTB33 ZNF3 GATAD1 KLF16 NFIA NFIA TEAD1 SMAD4 ZFP1 HLF TFE3 BHLHE40 ZHX2 CEBPG KLF10 CEBPD CEBPD TEAD3 NFIL3 NFIL3 SOX13 SOX13 L3MBTL4 ZKSCAN8 ZKSCAN8 ATF4 ATF4 FOXP1 MBD1 TBX3 TBX3 GATAD2A ZGPAT FOXO3 FOXO3 TFAP4 HNF1A HNF1A DMAP1 DMAP1 TCF7 HMG20B HMG20B NCOR1 NCOR1 ARID4B ARID4B CREM ETS1 ETS1 NFIC NFIC SP1 HNF4G ZBTB33 KAT8 KAT8 JUND ZBTB26 ZBTB26 ZBTB26 CTCF KLF6 KLF6 THRB RXRB MAX ETV1 ETV1 TSC22D4 ELF1 ELF1 MYBL2 RXRA HOMEZ HOMEZ RCOR2 RCOR2 SMAD4 SMAD4 ATF1 REST REST REST ZSCAN2 ATF7 ATF7 ATF7 ZNF48 TGIF2 NFIA MYC ZNF580 ZNF792 YY1 YY1 ERG ZNF3 ZNF384 NFE2 RCOR2 SOX2 ZNF3
miRNA-GlycoEnzyme Interactome {ref. 9}
miRNA Name Pos. Context Score Frac. Wt. Context Frac. Conserved Quality
hsa-miR-6726-3p 477-484 -0.652 98.0 -0.652 99.0 noncon
hsa-miR-1293 731-738 -0.549 99.0 -0.549 99.0 noncon
hsa-miR-5008-5p 44-51 -0.484 99.0 -0.484 99.0 noncon
hsa-miR-4483 731-738 -0.465 99.0 -0.465 99.0 noncon
hsa-miR-8073 211-218 -0.411 99.0 -0.411 99.0 noncon
hsa-miR-4726-3p 183-190 -0.404 99.0 -0.404 99.0 noncon
hsa-miR-3173-5p 438-445 -0.402 98.0 -0.402 98.0 noncon
hsa-miR-6799-3p 438-445 -0.402 98.0 -0.402 98.0 noncon
hsa-miR-221-5p 211-218 -0.395 99.0 -0.395 99.0 noncon
hsa-miR-6724-5p 403-410 -0.386 97.0 -0.386 98.0 noncon
hsa-miR-6809-5p 351-358 -0.377 97.0 -0.377 98.0 noncon
hsa-miR-6840-5p 73-79 -0.371 94.0 -0.371 96.0 noncon
hsa-miR-6773-5p 403-410 -0.365 97.0 -0.365 97.0 noncon
hsa-miR-4267 413-420 -0.361 98.0 -0.361 98.0 noncon
hsa-miR-3940-3p 200-206 -0.352 93.0 -0.352 95.0 noncon
hsa-miR-6886-3p 453-460 -0.349 96.0 -0.349 97.0 noncon
hsa-miR-4530 149-156 -0.349 98.0 -0.349 98.0 noncon
hsa-miR-1249-3p 477-483 -0.34 94.0 -0.34 96.0 noncon
hsa-miR-361-3p 523-530 -0.326 97.0 -0.326 98.0 noncon
hsa-miR-140-3p.1 608-615 -0.323 98.0 -0.323 98.0 noncon
hsa-miR-4290 475-481 -0.311 91.0 -0.311 93.0 noncon
hsa-miR-4290 637-644 -0.31 91.0 -0.31 93.0 noncon
hsa-miR-5008-5p 140-147 -0.306 95.0 -0.306 96.0 noncon
hsa-miR-326 343-350 -0.301 97.0 -0.301 97.0 noncon
hsa-miR-644a 355-361 -0.292 94.0 -0.292 96.0 noncon
hsa-miR-363-5p 732-738 -0.292 95.0 -0.292 96.0 noncon
hsa-miR-6793-3p 314-321 -0.29 97.0 -0.29 98.0 noncon
hsa-miR-3688-5p 353-360 -0.288 95.0 -0.288 97.0 noncon
hsa-miR-330-5p 343-350 -0.28 96.0 -0.28 97.0 noncon
hsa-miR-876-3p 730-736 -0.277 93.0 -0.277 95.0 noncon
hsa-miR-758-3p 559-566 -0.275 96.0 -0.275 97.0 noncon
hsa-miR-4302 357-364 -0.273 96.0 -0.273 97.0 noncon
hsa-miR-3918 648-655 -0.269 94.0 -0.269 95.0 noncon
hsa-miR-1291 42-48 -0.269 91.0 -0.269 93.0 noncon
hsa-miR-4732-3p 447-454 -0.269 98.0 -0.269 98.0 noncon
hsa-miR-2467-5p 379-386 -0.268 96.0 -0.268 97.0 noncon
hsa-miR-6715b-5p 605-611 -0.268 97.0 -0.268 97.0 noncon
hsa-miR-7157-5p 757-763 -0.263 96.0 -0.263 97.0 noncon