FuncClass

GlycoEnzDB
Comprehensive glycoEnzyme database and dynamic pathway generation

Description: The GlycoEnzDB is a manually curated glycoEnzyme database, primarily focused on humans. It covers 390 enzymes across 28 pathway maps. Facilities are also available to create custom glycosylation reaction pathways using experimental data in SBML format and for pathway simulation
Contacts: Sriram Neelamegham (neel@buffalo.edu), Yusen Zhou (yusenzho@buffalo.edu) or Ted Groth (tgroth@buffalo.edu).

GlycoEnzDB
Create glycosylation pathway
Custom database generation
Instructions/Reference
 
Pathway Map
MFNG



General Information
Gene Symbol:   
MFNG
Protein name:   
Beta-1,3-N-acetylglucosaminyltransferase manic fringe
Enzyme Class:   
Glycosyltransferase
CAZy Family:   
GT31
E.C. No:   
2.4.1.222
Organs:   
Reaction (SNFG):   
+
→
+ UDP
Reaction (IUBMB):   
No specified reaction.
About

• Fringe catalyzes the addition of a β1,3GlcNAc linkage to O-fucose in certain Epidermal Growth Factor-like (EGF) repeats, for example in the extracellular domain of Notch. This then controls Notch dependent signaling.
• This structure may be further elongated to a tetrasaccharide with the structure NeuAcα2-3/6Galβ1-4GlcNAcβ1-3Fuc in mammals.
• Three vertebrate fringe homologues have been identified: Lunatic fringe (Lfng), Manic fringe (Mfng), and Radical fringe (Rfng).
• Since over 100 proteins contain O-fucose consensus sites in EGF repeats, fringe may modify O-fucose on a wide variety of proteins.

Related Pathway
KEGG ORTHOLOGY:   
K05948
KEGG Pathway:   
ko00514  ko04330  ko05165 
Reactome:   
R-HSA-1912420
Related Database
Organism:   
Homo sapiens
Uniprot:   
O00587 
NCBI GeneID:   
4242
GlyMap GeneID:   
4242
DNA aRefSeq:   
NM_001166343.1 
Protein RefSeq:   
NP_001159815.1 
BRENDA:   
Kinetic info.
OMIM number:   
602577
GeneCards database:   
MFNG
Enzyme Specificity
Glycan Type:  
     O-linked (other)
Compartment:  
     Cis, Medial, Trans, TGN
Substrate:  
     Fuc(a1-?)Ser|Thr
Product:  
     GlcNAc(b1-3)Fuc(a1-?)Ser|Thr
Simulation Parameters
Mechaelis-Menten parameters (suggested)
VM:       (pmol/cell • hr)       KM:       (pM)
Relative conc of enzymes in compartment.(sum will be normalized to 1)
ER Cis- Medial- Trans- TGN










Expression Data in Tissue {ref. 5} Source from NCBI gene database
adrenal appendix bone marrow brain colon duodenum endometrium esophagus fat gall bladder heart kidney liver lung lymph node ovary pancreas placenta prostate salivary gland skin small intestine spleen stomach testis thyroid urinary bladder 40 30 20 10 0 RPKM
Expression Data in Cells {ref. 13} Source from Cancer Cell Line Encyclopedia database
Kidney Lung Breast Prostate Large intestine Ovary HL60 haematopoietic and lymphoid tissue Skin Central nervous system 786O A498 ACHN CAKI1 UO31 A549 EKVX HOP62 HOP92 NCIH226 NCIH23 NCIH322 NCIH460 NCIH522 SHP77 BT549 HS578T MCF7 MDAMB231 MDAMB468 T47D DU145 PC3 HCT116 HCT15 HT29 KM12 SW620 OVCAR4 OVCAR8 IGROV1 SKOV3 HL60 K562 RPMI8226 LOXIMVI MALME3M RPMI7951 SKMEL28 SKMEL5 UACC257 UACC62 SF268 SF295 SF539 SNB75 160 140 120 100 80 60 40 20 0 Transcripts per million (TPM)
Transcription factor {ref. 10}
No high degree of confidence data
miRNA-GlycoEnzyme Interactome {ref. 9}
miRNA Name Pos. Context Score Frac. Wt. Context Frac. Conserved Quality
hsa-miR-6797-5p 847-854 -0.647 99.0 -0.647 99.0 noncon
hsa-miR-1249-5p 847-854 -0.631 99.0 -0.631 99.0 noncon
hsa-miR-6829-5p 740-747 -0.612 99.0 -0.612 99.0 noncon
hsa-miR-3691-5p 834-841 -0.508 98.0 -0.508 99.0 noncon
hsa-miR-6165 758-765 -0.486 99.0 -0.486 99.0 noncon
hsa-let-7g-3p 865-872 -0.475 99.0 -0.475 99.0 noncon
hsa-miR-4313 278-285 -0.472 98.0 -0.472 98.0 noncon
hsa-let-7a-2-3p 865-872 -0.459 99.0 -0.459 99.0 noncon
hsa-miR-7152-3p 552-559 -0.45 98.0 -0.45 99.0 noncon
hsa-miR-939-5p 531-538 -0.449 98.0 -0.449 99.0 noncon
hsa-miR-5189-3p 122-129 -0.444 98.0 -0.444 99.0 noncon
hsa-miR-342-5p 163-170 -0.441 98.0 -0.441 98.0 noncon
hsa-miR-2355-5p 726-733 -0.44 99.0 -0.44 99.0 noncon
hsa-miR-4782-3p 828-835 -0.439 98.0 -0.439 98.0 con
hsa-miR-219a-5p 828-835 -0.439 98.0 -0.439 98.0 con
hsa-miR-6766-3p 828-835 -0.439 98.0 -0.439 98.0 con
hsa-miR-1343-5p 531-538 -0.433 98.0 -0.433 99.0 noncon
hsa-miR-4727-5p 470-477 -0.419 98.0 -0.419 99.0 noncon
hsa-miR-4664-5p 163-170 -0.407 98.0 -0.407 98.0 noncon
hsa-miR-939-3p 131-138 -0.403 98.0 -0.403 99.0 noncon
hsa-miR-6890-3p 324-331 -0.38 99.0 -0.38 99.0 noncon
hsa-miR-6827-5p 50-57 -0.379 96.0 -0.379 97.0 noncon
hsa-miR-6769a-3p 665-672 -0.378 99.0 -0.378 99.0 noncon
hsa-miR-1225-3p 392-399 -0.366 97.0 -0.366 97.0 noncon
hsa-miR-296-5p 584-591 -0.365 94.0 -0.365 95.0 noncon
hsa-miR-4505 612-619 -0.349 97.0 -0.349 98.0 noncon
hsa-miR-5006-5p 75-82 -0.344 97.0 -0.344 98.0 noncon
hsa-miR-1225-3p 299-306 -0.341 96.0 -0.341 97.0 noncon
hsa-miR-3135b 850-857 -0.338 98.0 -0.338 99.0 noncon
hsa-miR-6842-3p 613-620 -0.336 97.0 -0.336 98.0 noncon
hsa-miR-6857-3p 377-384 -0.334 97.0 -0.334 98.0 noncon
hsa-miR-4769-3p 469-476 -0.322 98.0 -0.322 98.0 noncon
hsa-miR-4741 192-199 -0.316 97.0 -0.316 98.0 noncon
hsa-miR-3190-5p 688-695 -0.309 98.0 -0.309 98.0 noncon
hsa-miR-6817-5p 469-476 -0.306 98.0 -0.306 98.0 noncon
hsa-miR-6788-5p 515-522 -0.301 98.0 -0.301 98.0 noncon
hsa-miR-30c-1-3p 515-522 -0.301 98.0 -0.301 98.0 noncon
hsa-miR-6127 423-430 -0.294 94.0 -0.294 95.0 noncon
hsa-miR-887-5p 516-523 -0.293 96.0 -0.293 97.0 noncon
hsa-miR-30c-2-3p 515-522 -0.293 98.0 -0.293 98.0 noncon
hsa-miR-4505 646-652 -0.289 95.0 -0.289 96.0 noncon
hsa-miR-6133 423-430 -0.286 94.0 -0.286 95.0 noncon
hsa-miR-6810-3p 276-282 -0.283 93.0 -0.283 95.0 noncon
hsa-miR-196a-3p 502-509 -0.281 94.0 -0.281 95.0 noncon
hsa-miR-5002-3p 96-102 -0.28 92.0 -0.28 95.0 noncon
hsa-miR-6129 423-430 -0.278 94.0 -0.278 95.0 noncon
hsa-miR-331-3p 197-204 -0.277 97.0 -0.277 97.0 noncon
hsa-miR-6738-3p 471-477 -0.274 95.0 -0.274 96.0 noncon
hsa-miR-5787 612-619 -0.271 96.0 -0.271 97.0 noncon
hsa-miR-4510 423-430 -0.27 93.0 -0.27 94.0 noncon
hsa-miR-6515-5p 848-854 -0.267 93.0 -0.267 95.0 noncon
hsa-miR-4675 192-199 -0.267 98.0 -0.267 98.0 noncon
hsa-miR-6130 423-430 -0.262 92.0 -0.262 94.0 noncon
hsa-miR-4419a 423-430 -0.262 92.0 -0.262 94.0 noncon
hsa-miR-2467-5p 569-576 -0.258 95.0 -0.258 97.0 noncon
hsa-miR-6769a-3p 238-245 -0.255 97.0 -0.255 98.0 noncon
hsa-miR-4323 239-245 -0.253 94.0 -0.253 96.0 noncon
hsa-miR-1266-3p 547-554 -0.253 94.0 -0.253 96.0 noncon
hsa-miR-6801-3p 276-282 -0.251 91.0 -0.251 93.0 noncon
hsa-miR-3139 523-529 -0.25 94.0 -0.25 96.0 noncon
hsa-miR-4323 666-672 -0.25 94.0 -0.25 96.0 noncon