FuncClass

GlycoEnzDB
Comprehensive glycoEnzyme database and dynamic pathway generation

Description: The GlycoEnzDB is a manually curated glycoEnzyme database, primarily focused on humans. It covers 390 enzymes across 28 pathway maps. Facilities are also available to create custom glycosylation reaction pathways using experimental data in SBML format and for pathway simulation
Contacts: Sriram Neelamegham (neel@buffalo.edu), Yusen Zhou (yusenzho@buffalo.edu) or Ted Groth (tgroth@buffalo.edu).

GlycoEnzDB
Create glycosylation pathway
Custom database generation
Instructions/Reference
 
Pathway Map
MGAT2



General Information
Gene Symbol:   
MGAT2
Protein name:   
Alpha-1,6-mannosyl-glycoprotein 2-beta-N-acetylglucosaminyltransferase
Enzyme Class:   
Glycosyltransferase
CAZy Family:   
GT16
E.C. No:   
2.4.1.143
Organs:   
Reaction (SNFG):   
+
→
+ UDP
Reaction (IUBMB):   
No specified reaction.
About

• MGAT2 catalyzes the transfer of β1,2-linked GlcNAc from UDP-GlcNAc to the terminal α1,6-linked Man in N-glycans
• The enzyme does not require the core chitobiosyl, α1-6 Fuc or peptide backbone. It is inhibited by the presence of Gaβb1-4 (i.e. β1-4 Gal-T) and bisecting β1-4GlcNAc (i.e. GnT-III/MGAT3)
• It is ubiquitously expressed

Related Pathway
KEGG ORTHOLOGY:   
K00736
KEGG Pathway:   
ko00510  ko00513  ko01100 
Reactome:   
R-HSA-4793952
Related Database
Organism:   
Homo sapiens
Uniprot:   
Q10469 
NCBI GeneID:   
4247
GlyMap GeneID:   
4247
DNA aRefSeq:   
NM_002408.3 
Protein RefSeq:   
NP_002399.1 
BRENDA:   
Kinetic info.
OMIM number:   
602616
GeneCards database:   
MGAT2
Enzyme Specificity
Glycan Type:  
     N-linked
Compartment:  
     Cis, Medial, Trans, TGN
Substrate:  
     Man(a1-6)
Product:  
     GlcNAc(b1-2)Man(a1-6)
Constraints:  
     nMan(a1-?)=2&!GlcNAc(b1-4)...Man(b1-4)&!Fuc(a1-3)&!Gal(b1-?)
Simulation Parameters
Mechaelis-Menten parameters (suggested)
VM:    1.40e-04    (pmol/cell • hr)       KM:    1.90e+08    (pM)
Relative conc of enzymes in compartment.(sum will be normalized to 1)
ER Cis- Medial- Trans- TGN










Expression Data in Tissue {ref. 5} Source from NCBI gene database
No expression data available.
Expression Data in Cells {ref. 13} Source from Cancer Cell Line Encyclopedia database
Kidney Lung Breast Prostate Large intestine Ovary HL60 haematopoietic and lymphoid tissue Skin Central nervous system 786O A498 ACHN CAKI1 UO31 A549 EKVX HOP62 HOP92 NCIH226 NCIH23 NCIH322 NCIH460 NCIH522 SHP77 BT549 HS578T MCF7 MDAMB231 MDAMB468 T47D DU145 PC3 HCT116 HCT15 HT29 KM12 SW620 OVCAR4 OVCAR8 IGROV1 SKOV3 HL60 K562 RPMI8226 LOXIMVI MALME3M RPMI7951 SKMEL28 SKMEL5 UACC257 UACC62 SF268 SF295 SF539 SNB75 120 100 80 60 40 20 0 Transcripts per million (TPM)
Transcription factor {ref. 10}
No high degree of confidence data
miRNA-GlycoEnzyme Interactome {ref. 9}
miRNA Name Pos. Context Score Frac. Wt. Context Frac. Conserved Quality
hsa-miR-4718 340-347 -0.616 99.0 -0.257 95.0 noncon
hsa-miR-6826-3p 256-263 -0.595 99.0 -0.25 93.0 noncon
hsa-miR-3914 713-720 -0.583 99.0 -0.166 92.0 noncon
hsa-miR-7854-3p 629-636 -0.519 99.0 -0.219 94.0 noncon
hsa-miR-383-3p 675-682 -0.503 99.0 -0.213 96.0 noncon
hsa-miR-496.1 691-698 -0.502 99.0 -0.213 93.0 con
hsa-miR-181b-3p 764-771 -0.475 99.0 -0.138 91.0 noncon
hsa-miR-181b-2-3p 764-771 -0.475 99.0 -0.138 91.0 noncon
hsa-miR-4520-2-3p 43-50 -0.461 98.0 -0.461 98.0 noncon
hsa-miR-374b-3p 223-230 -0.459 99.0 -0.198 94.0 noncon
hsa-miR-320e 852-859 -0.455 99.0 -0.132 89.0 noncon
hsa-miR-4520-2-3p 125-132 -0.45 97.0 -0.194 87.0 noncon
hsa-miR-491-3p 703-710 -0.433 98.0 -0.129 83.0 noncon
hsa-miR-297 55-62 -0.414 98.0 -0.414 99.0 noncon
hsa-miR-4420 764-771 -0.41 99.0 -0.121 94.0 noncon
hsa-miR-200b-3p 521-528 -0.391 99.0 -0.171 95.0 con
hsa-miR-200c-3p 521-528 -0.391 99.0 -0.171 95.0 con
hsa-miR-4763-5p 1250-1257 -0.388 94.0 -0.004 28.0 noncon
hsa-miR-4790-5p 15-21 -0.38 94.0 -0.38 96.0 noncon
hsa-miR-3660 502-509 -0.37 98.0 -0.162 91.0 noncon
hsa-miR-429 521-528 -0.367 99.0 -0.161 94.0 con
hsa-miR-941 1337-1343 -0.359 92.0 -0.003 23.0 noncon
hsa-miR-3942-5p 816-823 -0.357 99.0 -0.106 88.0 noncon
hsa-miR-4316 1952-1959 -0.357 98.0 -0.003 44.0 noncon
hsa-miR-4703-5p 816-823 -0.355 99.0 -0.106 88.0 noncon
hsa-miR-4436b-3p 1903-1910 -0.35 98.0 -0.003 32.0 noncon
hsa-miR-5002-3p 1522-1528 -0.346 95.0 -0.003 29.0 noncon
hsa-miR-3942-3p 296-303 -0.34 99.0 -0.15 95.0 noncon
hsa-miR-661 905-912 -0.338 98.0 -0.003 33.0 noncon
hsa-miR-584-3p 80-86 -0.338 97.0 -0.338 98.0 noncon
hsa-miR-6879-5p 1903-1910 -0.326 97.0 -0.003 32.0 noncon
hsa-miR-2909 1905-1912 -0.319 97.0 -0.003 44.0 noncon
hsa-miR-6735-5p 1903-1910 -0.318 97.0 -0.003 32.0 noncon
hsa-miR-595 437-444 -0.318 99.0 -0.141 95.0 noncon
hsa-miR-3177-5p 307-313 -0.318 91.0 -0.141 73.0 noncon
hsa-miR-4672 2114-2121 -0.314 98.0 -0.003 40.0 noncon
hsa-miR-3152-5p 1592-1599 -0.312 96.0 -0.003 35.0 noncon
hsa-miR-877-3p 253-259 -0.309 96.0 -0.137 87.0 noncon
hsa-miR-609 496-502 -0.308 94.0 -0.137 83.0 noncon
hsa-miR-3126-3p 2159-2165 -0.302 96.0 -0.003 41.0 noncon
hsa-miR-424-3p 1045-1052 -0.3 96.0 -0.003 32.0 noncon
hsa-miR-6795-3p 256-262 -0.299 98.0 -0.133 86.0 noncon
hsa-miR-3128 2157-2164 -0.294 97.0 -0.003 29.0 noncon
hsa-miR-570-3p 624-631 -0.292 98.0 -0.13 92.0 noncon
hsa-miR-5589-5p 850-856 -0.289 99.0 -0.087 91.0 noncon
hsa-miR-5691 2148-2155 -0.287 96.0 -0.003 37.0 noncon
hsa-miR-873-3p 1524-1531 -0.285 98.0 -0.003 44.0 noncon
hsa-miR-6854-5p 1771-1778 -0.285 97.0 -0.003 40.0 noncon
hsa-miR-4526 502-509 -0.284 98.0 -0.127 93.0 noncon
hsa-miR-193a-5p 2054-2061 -0.279 95.0 -0.003 27.0 noncon
hsa-miR-6839-3p 848-854 -0.275 94.0 -0.083 80.0 noncon
hsa-miR-3672 1525-1532 -0.273 97.0 -0.002 40.0 noncon
hsa-miR-3176 1782-1789 -0.272 96.0 -0.002 37.0 noncon
hsa-miR-6805-3p 2148-2155 -0.271 96.0 -0.002 39.0 noncon
hsa-miR-1537-5p 341-347 -0.269 91.0 -0.12 75.0 noncon
hsa-miR-1911-5p 1713-1719 -0.266 90.0 -0.002 23.0 noncon
hsa-miR-3922-3p 1782-1789 -0.262 96.0 -0.002 36.0 noncon
hsa-miR-431-5p 2052-2058 -0.26 94.0 -0.002 42.0 noncon
hsa-miR-4796-3p 318-324 -0.258 98.0 -0.116 94.0 noncon
hsa-miR-6892-5p 1129-1136 -0.252 94.0 -0.002 24.0 noncon
hsa-miR-942-5p 250-257 -0.25 96.0 -0.112 88.0 noncon
hsa-miR-4693-3p 426-432 -0.25 91.0 -0.112 78.0 noncon