FuncClass

GlycoEnzDB
Comprehensive glycoEnzyme database and dynamic pathway generation

Description: The GlycoEnzDB is a manually curated glycoEnzyme database, primarily focused on humans. It covers 390 enzymes across 28 pathway maps. Facilities are also available to create custom glycosylation reaction pathways using experimental data in SBML format and for pathway simulation
Contacts: Sriram Neelamegham (neel@buffalo.edu), Yusen Zhou (yusenzho@buffalo.edu) or Ted Groth (tgroth@buffalo.edu).

GlycoEnzDB
Create glycosylation pathway
Custom database generation
Instructions/Reference
 
Pathway Map
MGAT5B



General Information
Gene Symbol:   
MGAT5B
Protein name:   
Alpha-1,6-mannosylglycoprotein 6-beta-N-acetylglucosaminyltransferase B
Enzyme Class:   
Glycosyltransferase
CAZy Family:   
GT18
E.C. No:   
2.4.1.*
Organs:   
Reaction (SNFG):   
+
→
+ UDP
Reaction (IUBMB):   
No specified reaction.
About

• ~30 % of all O-linked glycans in the brain are O-mannose based. This is initiated by protein O-mannosyltransferase 1 (POMT1) and POMT 2, followed by POMGnT1 activity.
• A brain-specific β1,6-N-GlcNAcT (MGAT5B) has been cloned that transfers β1,6GlcNAc to a-mannose in GlcNAc-β1,2-Man-a sequence on both N-glycans and O-mannosyl glycans. In vivo this enzyme is dominantly considered to act on o-mannose glycans of the brain.
• Due to its activity, a variety of extended structures can be formed on the O-mannose backbone including sialylated glycans, glycans with HNK-1 epitope, and those with Lewis X structures.

Related Pathway
KEGG ORTHOLOGY:   
K09661
KEGG Pathway:   
ko00510  ko00515  ko01100 
Reactome:   
No Record
Related Database
Organism:   
Homo sapiens
Uniprot:   
Q3V5L5 
NCBI GeneID:   
146664
GlyMap GeneID:   
146664
DNA aRefSeq:   
NM_001199172.1 
Protein RefSeq:   
NP_001186101.1 
BRENDA:   
Kinetic info.
OMIM number:   
612441
GeneCards database:   
MGAT5B
Enzyme Specificity
Glycan Type:  
     O-linked (other)
Compartment:  
     Cis, Medial, Trans, TGN
Substrate:  
     GlcNAc(b1-2)Man(a1-?)Ser|Thr
Product:  
     GlcNAc(b1-2)[GlcNAc(b1-6)]Man(a1-?)Ser|Thr
Simulation Parameters
Mechaelis-Menten parameters (suggested)
VM:    1.00e-05    (pmol/cell • hr)       KM:    1.30e+08    (pM)
Relative conc of enzymes in compartment.(sum will be normalized to 1)
ER Cis- Medial- Trans- TGN










Expression Data in Tissue {ref. 5} Source from NCBI gene database
adrenal appendix bone marrow brain colon duodenum endometrium esophagus fat gall bladder heart kidney liver lung lymph node ovary pancreas placenta prostate salivary gland skin small intestine spleen stomach testis thyroid urinary bladder 8 6 4 2 0 RPKM
Expression Data in Cells {ref. 13} Source from Cancer Cell Line Encyclopedia database
Kidney Lung Breast Prostate Large intestine Ovary HL60 haematopoietic and lymphoid tissue Skin Central nervous system 786O A498 ACHN CAKI1 UO31 A549 EKVX HOP62 HOP92 NCIH226 NCIH23 NCIH322 NCIH460 NCIH522 SHP77 BT549 HS578T MCF7 MDAMB231 MDAMB468 T47D DU145 PC3 HCT116 HCT15 HT29 KM12 SW620 OVCAR4 OVCAR8 IGROV1 SKOV3 HL60 K562 RPMI8226 LOXIMVI MALME3M RPMI7951 SKMEL28 SKMEL5 UACC257 UACC62 SF268 SF295 SF539 SNB75 80 60 40 20 0 Transcripts per million (TPM)
Transcription factor {ref. 10}
Transcriptional start site (TSS) 0 200 -2000 -1800 -1600 -1400 -1200 -1000 -800 -600 -400 -200
ENST00000428789 ENST00000565043 ENST00000565675 ENST00000568598 POU5F1 MYCN GRHL2
miRNA-GlycoEnzyme Interactome {ref. 9}
miRNA Name Pos. Context Score Frac. Wt. Context Frac. Conserved Quality
hsa-miR-4737 94-101 -0.503 96.0 -0.503 97.0 noncon
hsa-miR-3130-3p 1541-1548 -0.43 99.0 0.0 0.0 noncon
hsa-miR-6885-5p 1052-1059 -0.409 91.0 -0.409 93.0 noncon
hsa-miR-328-5p 1052-1059 -0.363 91.0 -0.363 93.0 noncon
hsa-miR-744-5p 1192-1198 -0.358 90.0 -0.358 92.0 noncon
hsa-miR-3187-5p 1184-1191 -0.356 98.0 -0.356 99.0 noncon
hsa-miR-4755-3p 19-26 -0.353 97.0 -0.353 98.0 noncon
hsa-miR-5189-5p 1184-1191 -0.351 98.0 -0.351 99.0 noncon
hsa-miR-6860 1184-1191 -0.345 98.0 -0.345 99.0 noncon
hsa-miR-296-5p 348-355 -0.345 93.0 -0.345 95.0 noncon
hsa-miR-1254 1263-1270 -0.34 98.0 -0.34 98.0 noncon
hsa-miR-1285-3p 1184-1191 -0.335 98.0 -0.335 99.0 noncon
hsa-miR-4749-3p 576-583 -0.333 95.0 -0.333 96.0 noncon
hsa-miR-6766-5p 1234-1241 -0.332 94.0 -0.332 95.0 noncon
hsa-miR-6788-3p 339-346 -0.331 91.0 -0.331 94.0 noncon
hsa-miR-6756-5p 1234-1241 -0.321 92.0 -0.321 94.0 noncon
hsa-miR-34c-5p 1226-1233 -0.303 91.0 -0.303 93.0 con
hsa-miR-449b-5p 1226-1233 -0.295 90.0 -0.295 93.0 con
hsa-miR-4540 1443-1450 -0.292 94.0 -0.292 96.0 noncon
hsa-miR-6837-5p 461-468 -0.288 95.0 -0.288 96.0 noncon
hsa-miR-34a-5p 1226-1233 -0.285 90.0 -0.285 92.0 con
hsa-miR-589-5p 1435-1442 -0.283 96.0 -0.283 97.0 noncon
hsa-miR-3116 1263-1270 -0.282 97.0 -0.282 98.0 noncon
hsa-miR-1247-3p 1307-1314 -0.277 91.0 -0.277 93.0 noncon
hsa-miR-4685-5p 461-468 -0.275 94.0 -0.275 95.0 noncon
hsa-miR-3679-3p 1456-1463 -0.273 92.0 -0.273 94.0 noncon
hsa-miR-6850-3p 322-328 -0.273 91.0 -0.273 93.0 noncon
hsa-miR-612 1184-1191 -0.267 98.0 -0.267 98.0 noncon
hsa-miR-138-5p 60-67 -0.265 90.0 -0.265 92.0 con
hsa-miR-6776-5p 1236-1242 -0.26 95.0 -0.26 96.0 noncon
hsa-miR-6804-3p 1540-1546 -0.256 91.0 0.0 0.0 noncon