FuncClass

GlycoEnzDB
Comprehensive glycoEnzyme database and dynamic pathway generation

Description: The GlycoEnzDB is a manually curated glycoEnzyme database, primarily focused on humans. It covers 390 enzymes across 28 pathway maps. Facilities are also available to create custom glycosylation reaction pathways using experimental data in SBML format and for pathway simulation
Contacts: Sriram Neelamegham (neel@buffalo.edu), Yusen Zhou (yusenzho@buffalo.edu) or Ted Groth (tgroth@buffalo.edu).

GlycoEnzDB
Create glycosylation pathway
Custom database generation
Instructions/Reference
 
Pathway Map
OGT



General Information
Gene Symbol:   
OGT
Protein name:   
UDP-N-acetylglucosamine--peptide N-acetylglucosaminyltransferase 110 kDa subunit
Enzyme Class:   
Glycosyltransferase
CAZy Family:   
GT41
E.C. No:   
2.4.1.255
Organs:   
Reaction (SNFG):   
+ Ser →
+ UDP
+ Thr →
+ UDP
Reaction (IUBMB):   
No specified reaction.
About

• 2-5% of cellular Glc is converted to UDP-GlcNAc. O-GlcNAc transferase (OGT) transfers this GlcNAc to a variety of substrates that regulate cell cycle progression, transcription, intracellular signaling, nutrient sensing, and neuronal plasticity. Such transfer typically takes place in protein regions that are non-structured.
• Due to its broad siubstrate specificity, this enzyme activity is associated with diabetes, neurodegenerative and oncology related disorders.
• The extent of O-GlcNAcylation is regulated by OGT and also an O-GlcNAc hydrolase (OGA), with the latter removing the GlcNAc residue.
• OGT is broadly distributed (particulalry in brain and pancrea) and it exists in three alternate spliced forms.

Related Pathway
KEGG ORTHOLOGY:   
K09667
KEGG Pathway:   
ko00514  ko04931 
Reactome:   
R-HSA-3214847
Related Database
Organism:   
Homo sapiens
Uniprot:   
O15294 
NCBI GeneID:   
8473
GlyMap GeneID:   
8473
DNA aRefSeq:   
NM_181672.2 
Protein RefSeq:   
NP_858058.1 
BRENDA:   
Kinetic info.
OMIM number:   
300255
GeneCards database:   
OGT
Enzyme Specificity
Glycan Type:  
     O-linked (other)
Compartment:  
     
Substrate:  
     Ser or Thr
Product:  
     GlcNAc(b1-?)Ser or GlcNAc(b1-?)Thr
Simulation Parameters
Mechaelis-Menten parameters (suggested)
VM:       (pmol/cell • hr)       KM:       (pM)
Relative conc of enzymes in compartment.(sum will be normalized to 1)
ER Cis- Medial- Trans- TGN










Expression Data in Tissue {ref. 5} Source from NCBI gene database
adrenal appendix bone marrow brain colon duodenum endometrium esophagus fat gall bladder heart kidney liver lung lymph node ovary pancreas placenta prostate salivary gland skin small intestine spleen stomach testis thyroid urinary bladder 80 60 40 20 0 RPKM
Expression Data in Cells {ref. 13} Source from Cancer Cell Line Encyclopedia database
Kidney Lung Breast Prostate Large intestine Ovary HL60 haematopoietic and lymphoid tissue Skin Central nervous system 786O A498 ACHN CAKI1 UO31 A549 EKVX HOP62 HOP92 NCIH226 NCIH23 NCIH322 NCIH460 NCIH522 SHP77 BT549 HS578T MCF7 MDAMB231 MDAMB468 T47D DU145 PC3 HCT116 HCT15 HT29 KM12 SW620 OVCAR4 OVCAR8 IGROV1 SKOV3 HL60 K562 RPMI8226 LOXIMVI MALME3M RPMI7951 SKMEL28 SKMEL5 UACC257 UACC62 SF268 SF295 SF539 SNB75 300 200 100 0 Transcripts per million (TPM)
Transcription factor {ref. 10}
No high degree of confidence data
miRNA-GlycoEnzyme Interactome {ref. 9}
miRNA Name Pos. Context Score Frac. Wt. Context Frac. Conserved Quality
hsa-miR-1199-3p 312-318 -0.417 97.0 -0.379 96.0 noncon
hsa-miR-361-3p 48-55 -0.398 98.0 -0.363 98.0 noncon
hsa-miR-2467-5p 35-42 -0.397 98.0 -0.362 98.0 noncon
hsa-miR-483-3p.2 785-792 -0.387 99.0 -0.185 95.0 con
hsa-miR-3162-3p 161-168 -0.374 95.0 -0.341 96.0 noncon
hsa-miR-7974 137-144 -0.369 96.0 -0.336 96.0 noncon
hsa-miR-593-5p 764-771 -0.35 95.0 -0.168 82.0 noncon
hsa-miR-6504-5p 139-146 -0.347 98.0 -0.316 98.0 con
hsa-miR-3679-3p 50-57 -0.337 95.0 -0.308 96.0 noncon
hsa-miR-3064-5p 139-146 -0.331 98.0 -0.302 97.0 con
hsa-miR-4772-3p 101-108 -0.331 99.0 -0.302 99.0 noncon
hsa-miR-6887-3p 291-298 -0.326 93.0 -0.297 93.0 noncon
hsa-miR-7851-3p 1021-1028 -0.323 96.0 -0.14 84.0 noncon
hsa-miR-770-5p 375-382 -0.323 97.0 -0.294 97.0 noncon
hsa-miR-4712-5p 375-382 -0.323 97.0 -0.294 97.0 noncon
hsa-miR-1271-5p 766-773 -0.318 95.0 -0.154 88.0 con
hsa-miR-3616-3p 1794-1801 -0.317 94.0 -0.137 76.0 noncon
hsa-miR-140-5p 544-550 -0.311 96.0 -0.28 96.0 con
hsa-miR-6881-5p 21-27 -0.306 91.0 -0.282 92.0 noncon
hsa-miR-96-5p 766-773 -0.305 95.0 -0.148 88.0 con
hsa-miR-6895-3p 167-174 -0.303 96.0 -0.277 96.0 noncon
hsa-miR-502-3p 235-242 -0.303 96.0 -0.277 97.0 con
hsa-miR-501-3p 235-242 -0.303 96.0 -0.277 97.0 con
hsa-miR-20b-3p 2041-2048 -0.297 97.0 -0.039 72.0 noncon
hsa-miR-3682-5p 2019-2026 -0.281 96.0 -0.122 88.0 noncon
hsa-miR-619-3p 1923-1930 -0.278 98.0 -0.121 92.0 noncon
hsa-miR-339-5p 503-510 -0.278 94.0 -0.25 94.0 noncon
hsa-miR-1226-5p 1795-1802 -0.273 96.0 -0.119 87.0 noncon
hsa-miR-7109-3p 1335-1342 -0.27 96.0 -0.118 88.0 noncon
hsa-miR-1265 735-742 -0.266 96.0 -0.13 91.0 noncon
hsa-miR-202-5p 1291-1298 -0.262 96.0 -0.115 86.0 con
hsa-miR-1226-3p 515-521 -0.26 95.0 -0.234 95.0 noncon
hsa-miR-3650 754-761 -0.254 93.0 -0.124 86.0 noncon
hsa-miR-6512-5p 120-127 -0.252 97.0 -0.23 97.0 noncon
hsa-miR-5579-3p 455-462 -0.251 96.0 -0.226 96.0 noncon