FuncClass

GlycoEnzDB
Comprehensive glycoEnzyme database and dynamic pathway generation

Description: The GlycoEnzDB is a manually curated glycoEnzyme database, primarily focused on humans. It covers 390 enzymes across 28 pathway maps. Facilities are also available to create custom glycosylation reaction pathways using experimental data in SBML format and for pathway simulation
Contacts: Sriram Neelamegham (neel@buffalo.edu), Yusen Zhou (yusenzho@buffalo.edu) or Ted Groth (tgroth@buffalo.edu).

GlycoEnzDB
Create glycosylation pathway
Custom database generation
Instructions/Reference
 
Pathway Map
POMT1



General Information
Gene Symbol:   
POMT1
Protein name:   
Protein O-mannosyl-transferase 1
Enzyme Class:   
Glycosyltransferase
CAZy Family:   
GT39
E.C. No:   
2.4.1.109
Organs:   
Reaction (SNFG):   
+ Ser →
+ UDP
+ Thr →
+ UDP
Reaction (IUBMB):   
No specified reaction.
About

• POMT1/POMT2 enzymes initiate O-Man biosynthesis at unfolded regions (mucin-like domain) of alpha-dystroglycan. Protein context appears to be important for directing coreM1-3 biosynthesis.
• These are unusual ER localized enzymes with 7 & 9 transmembrane helices.
• Defects in O-mannosyl glycans on α-DG are reported to cause several a-dystroglycanopathies.

Related Pathway
KEGG ORTHOLOGY:   
K00728
KEGG Pathway:   
ko00514  ko00515  ko01100 
Reactome:   
R-HSA-5083629
Related Database
Organism:   
Homo sapiens
Uniprot:   
Q9Y6A1 
NCBI GeneID:   
10585
GlyMap GeneID:   
10585
DNA aRefSeq:   
NM_001077365.1 
Protein RefSeq:   
NP_001070833.1 
BRENDA:   
Kinetic info.
OMIM number:   
607423
GeneCards database:   
POMT1
Enzyme Specificity
Glycan Type:  
     O-linked (other)
Compartment:  
     ER
Substrate:  
     Ser or Thr
Product:  
     Man(a1-?)Ser or Man(a1-?)Thr
Simulation Parameters
Mechaelis-Menten parameters (suggested)
VM:       (pmol/cell • hr)       KM:       (pM)
Relative conc of enzymes in compartment.(sum will be normalized to 1)
ER Cis- Medial- Trans- TGN










Expression Data in Tissue {ref. 5} Source from NCBI gene database
adrenal appendix bone marrow brain colon duodenum endometrium esophagus fat gall bladder heart kidney liver lung lymph node ovary pancreas placenta prostate salivary gland skin small intestine spleen stomach testis thyroid urinary bladder 30 20 10 0 RPKM
Expression Data in Cells {ref. 13} Source from Cancer Cell Line Encyclopedia database
Kidney Lung Breast Prostate Large intestine Ovary HL60 haematopoietic and lymphoid tissue Skin Central nervous system 786O A498 ACHN CAKI1 UO31 A549 EKVX HOP62 HOP92 NCIH226 NCIH23 NCIH322 NCIH460 NCIH522 SHP77 BT549 HS578T MCF7 MDAMB231 MDAMB468 T47D DU145 PC3 HCT116 HCT15 HT29 KM12 SW620 OVCAR4 OVCAR8 IGROV1 SKOV3 HL60 K562 RPMI8226 LOXIMVI MALME3M RPMI7951 SKMEL28 SKMEL5 UACC257 UACC62 SF268 SF295 SF539 SNB75 60 50 40 30 20 10 0 Transcripts per million (TPM)
Transcription factor {ref. 10}
No high degree of confidence data
miRNA-GlycoEnzyme Interactome {ref. 9}
miRNA Name Pos. Context Score Frac. Wt. Context Frac. Conserved Quality
hsa-miR-6761-3p 265-272 -0.68 98.0 -0.676 99.0 noncon
hsa-miR-4638-5p 289-296 -0.577 98.0 -0.573 99.0 noncon
hsa-miR-218-1-3p 681-688 -0.513 99.0 -0.51 99.0 noncon
hsa-miR-6744-5p 631-638 -0.407 98.0 -0.405 99.0 noncon
hsa-miR-4462 217-223 -0.395 95.0 -0.395 96.0 noncon
hsa-miR-4757-5p 257-264 -0.366 98.0 -0.364 98.0 noncon
hsa-miR-6859-3p 140-146 -0.352 95.0 -0.352 96.0 noncon
hsa-miR-4691-3p 592-599 -0.338 98.0 -0.336 98.0 noncon
hsa-miR-609 395-402 -0.327 95.0 -0.325 96.0 noncon
hsa-miR-6755-3p 278-285 -0.327 94.0 -0.325 96.0 noncon
hsa-miR-3934-3p 407-414 -0.32 97.0 -0.318 98.0 noncon
hsa-miR-1307-3p 290-296 -0.314 97.0 -0.312 98.0 noncon
hsa-miR-6503-5p 322-329 -0.311 95.0 -0.309 96.0 noncon
hsa-miR-6775-3p 300-307 -0.31 93.0 -0.308 95.0 noncon
hsa-miR-4527 322-329 -0.301 95.0 -0.299 96.0 noncon
hsa-miR-1273g-3p 308-315 -0.298 97.0 -0.296 98.0 noncon
hsa-miR-507 181-187 -0.297 98.0 -0.297 99.0 noncon
hsa-miR-5701 202-209 -0.293 97.0 -0.293 98.0 noncon
hsa-miR-3907 167-173 -0.289 93.0 -0.289 95.0 noncon
hsa-miR-6744-3p 257-264 -0.288 97.0 -0.286 98.0 noncon
hsa-miR-5008-5p 120-126 -0.282 94.0 -0.282 96.0 noncon
hsa-miR-3622b-5p 646-652 -0.28 95.0 -0.278 96.0 noncon
hsa-miR-6772-5p 396-402 -0.269 93.0 -0.268 95.0 noncon
hsa-miR-4640-5p 536-542 -0.269 90.0 -0.268 92.0 noncon
hsa-miR-5589-5p 398-405 -0.266 98.0 -0.265 99.0 noncon
hsa-miR-6878-3p 258-264 -0.26 93.0 -0.259 95.0 noncon
hsa-miR-1291 300-307 -0.26 90.0 -0.259 93.0 noncon
hsa-miR-6072 39-45 -0.255 93.0 -0.255 95.0 noncon
hsa-miR-6891-3p 39-45 -0.252 93.0 -0.252 95.0 noncon