FuncClass

GlycoEnzDB
Comprehensive glycoEnzyme database and dynamic pathway generation

Description: The GlycoEnzDB is a manually curated glycoEnzyme database, primarily focused on humans. It covers 390 enzymes across 28 pathway maps. Facilities are also available to create custom glycosylation reaction pathways using experimental data in SBML format and for pathway simulation
Contacts: Sriram Neelamegham (neel@buffalo.edu), Yusen Zhou (yusenzho@buffalo.edu) or Ted Groth (tgroth@buffalo.edu).

GlycoEnzDB
Create glycosylation pathway
Custom database generation
Instructions/Reference
 
Pathway Map
ST6GAL2



General Information
Gene Symbol:   
ST6GAL2
Protein name:   
Beta-galactoside alpha-2,6-sialyltransferase 2
Enzyme Class:   
Glycosyltransferase
CAZy Family:   
GT29
E.C. No:   
2.4.99.1
Organs:   
Reaction (SNFG):   
+
→
+ CMP
+
→
+ CMP
Reaction (IUBMB):   
No specified reaction.
About

• ST6Gal-II utilizes the LacdiNAc structure [GalNAc(β1-4)GlcNAc] as a preferred acceptor substrate over the Gal(β1-4)GlcNAc structure.
• ST6Gal-II gene is expressed in tissue- and stage-specific manner.
• It is expressed in brain, lymph node, testis, thyroid gland, and fetal tissues.
• ST6Gal-II prefers oligosaccharides to glycoproteins, whereas ST6Gal-I prefers glycoproteins to oligosaccharides as acceptor substrates. ST6Gal-2 does not act on glycolipids.

Related Pathway
KEGG ORTHOLOGY:   
K00779
KEGG Pathway:   
ko00510  ko00514  ko01100 
Reactome:   
R-HSA-4085001
Related Database
Organism:   
Homo sapiens
Uniprot:   
Q96JF0 
NCBI GeneID:   
84620
GlyMap GeneID:   
84620
DNA aRefSeq:   
NM_001142351.1 
Protein RefSeq:   
NP_001135823.1 
BRENDA:   
Kinetic info.
OMIM number:   
608472
GeneCards database:   
ST6GAL2
Enzyme Specificity
Glycan Type:  
     N-linked, O-linked (mucin type), O-linked (other)
Compartment:  
     
Substrate:  
     GalNAc(b1-4)GlcNAc(b1-?) or Gal(b1-4)GlcNAc(b1-?)
Product:  
     NeuAc(a2-6)GalNAc(b1-4)GlcNAc(b1-?) or NeuAc(a2-6)Gal(b1-4)GlcNAc(b1-?)
Simulation Parameters
Mechaelis-Menten parameters (suggested)
VM:    7.26e-05    (pmol/cell • hr)       KM:    2.60e+08    (pM)
Relative conc of enzymes in compartment.(sum will be normalized to 1)
ER Cis- Medial- Trans- TGN










Expression Data in Tissue {ref. 5} Source from NCBI gene database
adrenal appendix bone marrow brain colon duodenum endometrium esophagus fat gall bladder heart kidney liver lung lymph node ovary pancreas placenta prostate salivary gland skin small intestine spleen stomach testis thyroid urinary bladder 20 10 0 RPKM
Expression Data in Cells {ref. 13} Source from Cancer Cell Line Encyclopedia database
Kidney Lung Breast Prostate Large intestine Ovary HL60 haematopoietic and lymphoid tissue Skin Central nervous system 786O A498 ACHN CAKI1 UO31 A549 EKVX HOP62 HOP92 NCIH226 NCIH23 NCIH322 NCIH460 NCIH522 SHP77 BT549 HS578T MCF7 MDAMB231 MDAMB468 T47D DU145 PC3 HCT116 HCT15 HT29 KM12 SW620 OVCAR4 OVCAR8 IGROV1 SKOV3 HL60 K562 RPMI8226 LOXIMVI MALME3M RPMI7951 SKMEL28 SKMEL5 UACC257 UACC62 SF268 SF295 SF539 SNB75 30 20 10 0 Transcripts per million (TPM)
Transcription factor {ref. 10}
No high degree of confidence data
miRNA-GlycoEnzyme Interactome {ref. 9}
miRNA Name Pos. Context Score Frac. Wt. Context Frac. Conserved Quality
hsa-miR-4305 5070-5077 -0.519 99.0 -0.519 99.0 noncon
hsa-miR-519e-3p 4586-4593 -0.441 98.0 -0.441 99.0 noncon
hsa-miR-4641 4118-4125 -0.406 97.0 -0.406 98.0 noncon
hsa-miR-4730 4510-4517 -0.403 97.0 -0.403 97.0 noncon
hsa-miR-4460 4440-4447 -0.399 96.0 -0.399 97.0 noncon
hsa-miR-33b-3p 4586-4593 -0.396 97.0 -0.396 98.0 noncon
hsa-miR-25-5p 4509-4516 -0.374 94.0 -0.374 95.0 noncon
hsa-miR-210-3p 3731-3738 -0.362 93.0 -0.362 95.0 noncon
hsa-miR-515-3p 4586-4593 -0.347 98.0 -0.347 99.0 noncon
hsa-miR-363-3p 23-30 -0.335 97.0 -0.335 97.0 con
hsa-miR-25-3p 23-30 -0.322 97.0 -0.322 97.0 con
hsa-miR-367-3p 23-30 -0.319 96.0 -0.319 97.0 con
hsa-miR-92a-3p 23-30 -0.317 97.0 -0.317 97.0 con
hsa-miR-92b-3p 23-30 -0.317 97.0 -0.317 97.0 con
hsa-miR-574-5p 4528-4535 -0.316 94.0 -0.316 95.0 noncon
hsa-miR-487a-3p 4883-4890 -0.313 98.0 -0.313 99.0 con
hsa-miR-154-3p 4883-4890 -0.313 98.0 -0.313 99.0 con
hsa-miR-140-5p 4439-4446 -0.302 96.0 -0.302 97.0 noncon
hsa-miR-32-5p 23-30 -0.293 96.0 -0.293 97.0 con
hsa-miR-1205 839-846 -0.291 98.0 -0.291 98.0 noncon
hsa-miR-5187-5p 1234-1241 -0.289 94.0 -0.289 96.0 noncon
hsa-miR-22-3p 1215-1222 -0.281 94.0 -0.281 95.0 noncon
hsa-miR-887-3p 4164-4171 -0.277 91.0 -0.277 94.0 noncon
hsa-miR-219b-5p 401-408 -0.273 92.0 -0.273 94.0 noncon
hsa-miR-659-5p 647-654 -0.272 93.0 -0.272 96.0 noncon
hsa-miR-199b-3p 35-41 -0.27 94.0 -0.27 95.0 con
hsa-miR-199a-3p 35-41 -0.27 94.0 -0.27 95.0 con
hsa-miR-4535 648-655 -0.26 95.0 -0.26 96.0 noncon
hsa-miR-7153-3p 1395-1402 -0.257 96.0 -0.257 97.0 noncon
hsa-miR-758-3p 53-60 -0.256 96.0 -0.256 97.0 con