FuncClass

GlycoEnzDB
Comprehensive glycoEnzyme database and dynamic pathway generation

Description: The GlycoEnzDB is a manually curated glycoEnzyme database, primarily focused on humans. It covers 390 enzymes across 28 pathway maps. Facilities are also available to create custom glycosylation reaction pathways using experimental data in SBML format and for pathway simulation
Contacts: Sriram Neelamegham (neel@buffalo.edu), Yusen Zhou (yusenzho@buffalo.edu) or Ted Groth (tgroth@buffalo.edu).

GlycoEnzDB
Create glycosylation pathway
Custom database generation
Instructions/Reference
 
Pathway Map
ST8SIA4



General Information
Gene Symbol:   
ST8SIA4
Protein name:   
CMP-N-acetylneuraminate-poly-alpha-2,8-sialyltransferase
Enzyme Class:   
Glycosyltransferase
CAZy Family:   
GT29
E.C. No:   
2.4.99.8
Organs:   
Reaction (SNFG):   
+
→
+ CMP
Reaction (IUBMB):   
No specified reaction.
About

• Polysialyltransferase ST8SIA4 adds sialic acids by α2,8-linkage on α2,3- or α2,6-linked sialic acid
• Polysialic acid on NCAM in the brain is synthesized by two polysialyltransferases, ST8SIA2 and ST8SIA4
• The majority of polysialic acid expressed in adult brain is lost in ST8SIA4-deficient mice, but polysialic acid is remained in premature neurons and migrating neurons expressing ST8SIA2. Lack of polysialic acid in double-knockout mice causes severe abnormalities in brain development such as neural cell death, aberrant neural cell migration, and differentiation.
• Polysialic acid is detected in many cancer cell lines. ST8SIA4 mRNA is detectable in human neuroblastoma, small and non-small cell lung carcinoma, and glioma. Here, polySia is thought to affect adhesive property of tumor cells and promote metastasis and migration of cancer cells in vivo. Thus, polysialic acid regulation may be an important target on tumor cells.
• Restricted to selected proteins (NCAM, SynCAM-1)

Related Pathway
KEGG ORTHOLOGY:   
K06614
KEGG Pathway:   
No Record 
Reactome:   
R-HSA-4085001
Related Database
Organism:   
Homo sapiens
Uniprot:   
Q92187 
NCBI GeneID:   
7903
GlyMap GeneID:   
7903
DNA aRefSeq:   
NM_005668.5 
Protein RefSeq:   
NP_005659.1 
BRENDA:   
Kinetic info.
OMIM number:   
602547
GeneCards database:   
ST8SIA4
Enzyme Specificity
Glycan Type:  
     N-linked, O-linked (mucin type)
Compartment:  
     
Substrate:  
     NeuAc(a2-?)
Product:  
     NeuAc(a2-8)NeuAc(a2-?)
Simulation Parameters
Mechaelis-Menten parameters (suggested)
VM:       (pmol/cell • hr)       KM:       (pM)
Relative conc of enzymes in compartment.(sum will be normalized to 1)
ER Cis- Medial- Trans- TGN










Expression Data in Tissue {ref. 5} Source from NCBI gene database
adrenal appendix bone marrow brain colon duodenum endometrium esophagus fat gall bladder heart kidney liver lung lymph node ovary pancreas placenta prostate salivary gland skin small intestine spleen stomach testis thyroid urinary bladder 8 6 4 2 0 RPKM
Expression Data in Cells {ref. 13} Source from Cancer Cell Line Encyclopedia database
Kidney Lung Breast Prostate Large intestine Ovary HL60 haematopoietic and lymphoid tissue Skin Central nervous system 786O A498 ACHN CAKI1 UO31 A549 EKVX HOP62 HOP92 NCIH226 NCIH23 NCIH322 NCIH460 NCIH522 SHP77 BT549 HS578T MCF7 MDAMB231 MDAMB468 T47D DU145 PC3 HCT116 HCT15 HT29 KM12 SW620 OVCAR4 OVCAR8 IGROV1 SKOV3 HL60 K562 RPMI8226 LOXIMVI MALME3M RPMI7951 SKMEL28 SKMEL5 UACC257 UACC62 SF268 SF295 SF539 SNB75 20 10 0 Transcripts per million (TPM)
Transcription factor {ref. 10}
No high degree of confidence data
miRNA-GlycoEnzyme Interactome {ref. 9}
miRNA Name Pos. Context Score Frac. Wt. Context Frac. Conserved Quality
hsa-miR-125b-5p 2924-2931 -0.385 97.0 -0.385 98.0 con
hsa-miR-3668 3578-3585 -0.374 98.0 -0.374 98.0 noncon
hsa-miR-4319 2924-2931 -0.369 97.0 -0.369 98.0 con
hsa-miR-125a-5p 2924-2931 -0.361 97.0 -0.361 98.0 con
hsa-miR-541-3p 3932-3938 -0.354 97.0 -0.354 97.0 noncon
hsa-miR-193b-5p 4825-4832 -0.337 94.0 -0.337 96.0 noncon
hsa-miR-6814-5p 1968-1975 -0.333 98.0 -0.333 99.0 noncon
hsa-miR-654-5p 3932-3938 -0.332 96.0 -0.332 97.0 noncon
hsa-miR-124-3p.1 2794-2800 -0.328 96.0 -0.328 97.0 con
hsa-miR-1178-3p 3417-3424 -0.327 97.0 -0.327 98.0 noncon
hsa-miR-1263 1122-1129 -0.325 94.0 -0.325 96.0 noncon
hsa-miR-1909-5p 2298-2305 -0.324 95.0 -0.324 97.0 noncon
hsa-miR-1207-3p 144-151 -0.317 98.0 -0.317 98.0 noncon
hsa-miR-183-5p.2 209-215 -0.316 95.0 -0.316 96.0 con
hsa-miR-3614-3p 3088-3095 -0.311 97.0 -0.311 98.0 noncon
hsa-miR-4735-3p 4364-4370 -0.301 96.0 -0.301 97.0 noncon
hsa-miR-6500-3p 4905-4912 -0.294 98.0 -0.294 98.0 noncon
hsa-miR-18b-5p 4364-4370 -0.289 95.0 -0.289 97.0 noncon
hsa-miR-18a-5p 4364-4370 -0.289 95.0 -0.289 97.0 noncon
hsa-miR-541-5p 4845-4852 -0.288 97.0 -0.288 98.0 noncon
hsa-miR-3944-5p 4461-4468 -0.284 95.0 -0.284 97.0 noncon
hsa-miR-4793-3p 1446-1453 -0.283 97.0 -0.283 98.0 noncon
hsa-miR-7112-3p 4002-4009 -0.28 95.0 -0.28 96.0 noncon
hsa-miR-553 4380-4386 -0.279 91.0 -0.279 94.0 noncon
hsa-miR-630 225-232 -0.279 94.0 -0.279 96.0 noncon
hsa-miR-7843-3p 4289-4296 -0.275 95.0 -0.275 96.0 noncon
hsa-miR-218-5p 4401-4408 -0.269 94.0 -0.269 95.0 con
hsa-miR-5189-3p 4763-4770 -0.268 93.0 -0.268 95.0 noncon
hsa-miR-4656 3455-3462 -0.267 95.0 -0.267 96.0 noncon
hsa-miR-324-5p 559-565 -0.265 91.0 -0.265 94.0 noncon
hsa-miR-3170 4826-4832 -0.262 94.0 -0.262 96.0 noncon
hsa-miR-6832-5p 3825-3832 -0.261 92.0 -0.261 95.0 noncon
hsa-miR-208b-5p 643-650 -0.258 97.0 -0.258 98.0 noncon
hsa-miR-371b-3p 2297-2303 -0.257 92.0 -0.257 94.0 noncon
hsa-miR-1206 4811-4817 -0.257 95.0 -0.257 97.0 noncon
hsa-miR-5195-5p 3619-3626 -0.254 93.0 -0.254 95.0 noncon
hsa-miR-3938 4888-4895 -0.253 94.0 -0.253 96.0 noncon