FuncClass

GlycoEnzDB
Comprehensive glycoEnzyme database and dynamic pathway generation

Description: The GlycoEnzDB is a manually curated glycoEnzyme database, primarily focused on humans. It covers 390 enzymes across 28 pathway maps. Facilities are also available to create custom glycosylation reaction pathways using experimental data in SBML format and for pathway simulation
Contacts: Sriram Neelamegham (neel@buffalo.edu), Yusen Zhou (yusenzho@buffalo.edu) or Ted Groth (tgroth@buffalo.edu).

GlycoEnzDB
Create glycosylation pathway
Custom database generation
Instructions/Reference
 
Pathway Map
UGGT2



General Information
Gene Symbol:   
UGGT2
Protein name:   
UDP-glucose:glycoprotein glucosyltransferase 2
Enzyme Class:   
Glycosyltransferase
CAZy Family:   
GT24
E.C. No:   
2.4.1.*
Organs:   
Reaction (SNFG):   
+
→
+ UDP
Reaction (IUBMB):   
No specified reaction.
About

• UGGT is a large soluble protein (150-170 kDa) that is mostly ER retained.
• This is the glycoprotein folding sensor enzyme. If the glycoprotein is incompletely folded, the misfolded protein is recognized in the context of its N-glycan by the folding sensor UGGT. UGGT puts back one α(1-3)Glc, and refolding is again attempted by CNX/CRT.
• Proteins unable to properly fold after multiple cycles are eventually retro-translocated to the cytosol to be degraded by the proteasome, a process known as endoplasmic reticulum-associated degradation (ERAD).
• It is currently thought that the N-terminal may bind the misfolded polypeptide, while the C-terminal performs glycosyltransferase activity. In humans there are two proteins mediating this enzyme activity.
• Although extensively distributed in nature, UGGT is not required for single cell viability under normal conditions.

Related Pathway
KEGG ORTHOLOGY:   
K11718
KEGG Pathway:   
ko04141 
Reactome:   
R-HSA-901032
Related Database
Organism:   
Homo sapiens
Uniprot:   
Q9NYU1 
NCBI GeneID:   
55757
GlyMap GeneID:   
55757
DNA aRefSeq:   
NM_020121.3 
Protein RefSeq:   
NP_064506.3 
BRENDA:   
Kinetic info.
OMIM number:   
605898
GeneCards database:   
UGGT2
Enzyme Specificity
Glycan Type:  
     N-linked
Compartment:  
     ER
Substrate:  
     Man(a1-2)Man(a1-2)Man(a1-3)
Product:  
     Glc(a1-3)Man(a1-2)Man(a1-2)Man(a1-3)
Constraints:  
     nMan(a1-?)<9
Simulation Parameters
Mechaelis-Menten parameters (suggested)
VM:       (pmol/cell • hr)       KM:       (pM)
Relative conc of enzymes in compartment.(sum will be normalized to 1)
ER Cis- Medial- Trans- TGN










Expression Data in Tissue {ref. 5} Source from NCBI gene database
adrenal appendix bone marrow brain colon duodenum endometrium esophagus fat gall bladder heart kidney liver lung lymph node ovary pancreas placenta prostate salivary gland skin small intestine spleen stomach testis thyroid urinary bladder 8 6 4 2 0 RPKM
Expression Data in Cells {ref. 13} Source from Cancer Cell Line Encyclopedia database
Kidney Lung Breast Prostate Large intestine Ovary HL60 haematopoietic and lymphoid tissue Skin Central nervous system 786O A498 ACHN CAKI1 UO31 A549 EKVX HOP62 HOP92 NCIH226 NCIH23 NCIH322 NCIH460 NCIH522 SHP77 BT549 HS578T MCF7 MDAMB231 MDAMB468 T47D DU145 PC3 HCT116 HCT15 HT29 KM12 SW620 OVCAR4 OVCAR8 IGROV1 SKOV3 HL60 K562 RPMI8226 LOXIMVI MALME3M RPMI7951 SKMEL28 SKMEL5 UACC257 UACC62 SF268 SF295 SF539 SNB75 40 30 20 10 0 Transcripts per million (TPM)
Transcription factor {ref. 10}
No high degree of confidence data
miRNA-GlycoEnzyme Interactome {ref. 9}
miRNA Name Pos. Context Score Frac. Wt. Context Frac. Conserved Quality
hsa-miR-6880-3p 20-27 -0.721 99.0 -0.721 99.0 noncon
hsa-miR-27a-5p 756-763 -0.497 99.0 -0.002 16.0 noncon
hsa-miR-6868-3p 15-22 -0.435 99.0 -0.435 99.0 noncon
hsa-miR-3128 255-262 -0.402 99.0 -0.001 26.0 noncon
hsa-miR-5692a 138-145 -0.399 99.0 -0.399 99.0 noncon
hsa-miR-4772-5p 228-235 -0.391 97.0 -0.001 23.0 noncon
hsa-miR-4729 186-193 -0.357 99.0 -0.357 99.0 noncon
hsa-miR-3126-3p 257-264 -0.343 97.0 -0.001 38.0 noncon
hsa-miR-410-3p 178-185 -0.342 99.0 -0.342 99.0 noncon
hsa-miR-4297 19-25 -0.32 94.0 -0.32 96.0 noncon
hsa-miR-5581-5p 19-25 -0.299 93.0 -0.299 95.0 noncon
hsa-miR-3691-5p 327-334 -0.288 93.0 -0.001 26.0 noncon
hsa-miR-4641 897-903 -0.286 92.0 -0.001 19.0 noncon
hsa-miR-4283 67-73 -0.284 92.0 -0.284 94.0 noncon
hsa-miR-4727-5p 478-485 -0.279 95.0 -0.001 22.0 noncon
hsa-miR-3937 47-53 -0.273 92.0 -0.273 95.0 noncon
hsa-miR-3686 684-691 -0.27 96.0 -0.001 22.0 noncon
hsa-miR-6758-3p 166-172 -0.267 97.0 -0.267 98.0 noncon
hsa-miR-1248 15-21 -0.266 96.0 -0.266 97.0 noncon
hsa-miR-5700 692-698 -0.261 97.0 -0.001 39.0 noncon
hsa-miR-186-5p 114-121 -0.26 99.0 -0.26 99.0 noncon
hsa-miR-6878-3p 421-428 -0.258 93.0 -0.001 36.0 noncon
hsa-miR-4524a-3p 654-661 -0.255 94.0 -0.001 30.0 noncon
hsa-miR-3686 704-711 -0.255 96.0 -0.001 22.0 noncon
hsa-miR-888-5p 197-203 -0.254 97.0 -0.254 98.0 noncon
hsa-miR-617 88-94 -0.25 92.0 -0.25 94.0 noncon