FuncClass

GlycoEnzDB
Comprehensive glycoEnzyme database and dynamic pathway generation

Description: The GlycoEnzDB is a manually curated glycoEnzyme database, primarily focused on humans. It covers 390 enzymes across 28 pathway maps. Facilities are also available to create custom glycosylation reaction pathways using experimental data in SBML format and for pathway simulation
Contacts: Sriram Neelamegham (neel@buffalo.edu), Yusen Zhou (yusenzho@buffalo.edu) or Ted Groth (tgroth@buffalo.edu).

GlycoEnzDB
Create glycosylation pathway
Custom database generation
Instructions/Reference
 
Pathway Map
HK1



General Information
Gene Symbol:   
HK1
Protein name:   
Hexokinase-1
Enzyme Class:   
CAZy Family:   
E.C. No:   
2.7.1.1
Organs:   
Reaction (SNFG):   

Glc or Fruc or GlcN or Man → Glc(6P) or Fruc(6P) or GlcN(6P) or Man(6P)
Reaction (IUBMB):   
No specified reaction.
About

• Hexokinase 1 phosphorylates various hexoses, such as glucose, fructose, and galactose in the cytoplasm
• It phosphorylates glucose to create glucose-6-phosphate. This is the first step of glycolysis, and necessary to form nucleotide-sugars.
• Does NOT phosphorylate D-Nacetylglucosamine (GlcNAc).
• This enzyme is responsible for the catalysis of the first event of glycolysis: the phosphorylation of glucose to glucose-1-phosphate.

Related Pathway
KEGG ORTHOLOGY:   
KEGG Pathway:   
No Record 
Reactome:   
R-HSA-5619056
Related Database
Organism:   
Homo sapiens
Uniprot:   
P19367 
NCBI GeneID:   
3098
GlyMap GeneID:   
3098
DNA aRefSeq:   
NM_000188.2 
Protein RefSeq:   
NP_000179.2 
BRENDA:   
Kinetic info.
OMIM number:   
142600
GeneCards database:   
HK1
Enzyme Specificity
Glycan Type:  
     
Compartment:  
     
Substrate:  
     Glc or Fruc or GlcN or Man
Product:  
     Glc(6P) or Fruc(6P) or GlcN(6P) or Man(6P)
Constraints:  
     None
Simulation Parameters
Mechaelis-Menten parameters (suggested)
VM:       (pmol/cell • hr)       KM:       (pM)
Relative conc of enzymes in compartment.(sum will be normalized to 1)
ER Cis- Medial- Trans- TGN










Expression Data in Tissue {ref. 5} Source from NCBI gene database
adrenal appendix bone marrow brain colon duodenum endometrium esophagus fat gall bladder heart kidney liver lung lymph node ovary pancreas placenta prostate salivary gland skin small intestine spleen stomach testis thyroid urinary bladder 50 40 30 20 10 0 RPKM
Expression Data in Cells {ref. 13} Source from Cancer Cell Line Encyclopedia database
Kidney Lung Breast Prostate Large intestine Ovary HL60 haematopoietic and lymphoid tissue Skin Central nervous system 786O A498 ACHN CAKI1 UO31 A549 EKVX HOP62 HOP92 NCIH226 NCIH23 NCIH322 NCIH460 NCIH522 SHP77 BT549 HS578T MCF7 MDAMB231 MDAMB468 T47D DU145 PC3 HCT116 HCT15 HT29 KM12 SW620 OVCAR4 OVCAR8 IGROV1 SKOV3 HL60 K562 RPMI8226 LOXIMVI MALME3M RPMI7951 SKMEL28 SKMEL5 UACC257 UACC62 SF268 SF295 SF539 SNB75 400 300 200 100 0 Transcripts per million (TPM)
Transcription factor {ref. 10}
No high degree of confidence data
miRNA-GlycoEnzyme Interactome {ref. 9}
miRNA Name Pos. Context Score Frac. Wt. Context Frac. Conserved Quality
hsa-miR-218-1-3p 657-664 -0.596 99.0 -0.596 99.0 noncon
hsa-miR-150-3p 734-741 -0.595 99.0 -0.594 99.0 noncon
hsa-miR-6886-5p 621-628 -0.567 98.0 -0.567 98.0 noncon
hsa-miR-1256 673-680 -0.546 99.0 -0.546 99.0 noncon
hsa-miR-4763-3p 213-220 -0.466 99.0 -0.466 99.0 noncon
hsa-miR-1207-5p 213-220 -0.464 99.0 -0.464 99.0 noncon
hsa-miR-6880-3p 450-457 -0.426 94.0 -0.426 95.0 noncon
hsa-miR-138-5p 407-414 -0.411 97.0 -0.411 97.0 con
hsa-miR-5689 284-291 -0.399 95.0 -0.399 97.0 noncon
hsa-miR-3186-3p 569-575 -0.388 91.0 -0.388 94.0 noncon
hsa-miR-3135b 28-35 -0.374 99.0 -0.374 99.0 noncon
hsa-miR-1256 530-537 -0.365 97.0 -0.365 98.0 noncon
hsa-miR-6732-5p 55-62 -0.364 97.0 -0.364 97.0 noncon
hsa-miR-3692-5p 409-416 -0.358 98.0 -0.358 98.0 noncon
hsa-miR-6786-5p 693-699 -0.321 93.0 -0.321 94.0 noncon
hsa-miR-149-3p 63-70 -0.315 96.0 -0.315 97.0 noncon
hsa-miR-4696 581-588 -0.308 97.0 -0.308 98.0 noncon
hsa-miR-6883-5p 63-70 -0.291 96.0 -0.291 96.0 noncon
hsa-miR-1263 735-741 -0.282 91.0 -0.282 94.0 noncon
hsa-miR-3144-5p 245-251 -0.28 91.0 -0.28 93.0 noncon
hsa-miR-6132 212-218 -0.277 93.0 -0.277 95.0 noncon
hsa-miR-4728-5p 63-70 -0.273 94.0 -0.273 95.0 noncon
hsa-miR-6836-5p 212-218 -0.262 92.0 -0.262 94.0 noncon
hsa-miR-6785-5p 63-70 -0.257 94.0 -0.257 95.0 noncon
hsa-miR-4515 559-565 -0.254 90.0 -0.254 93.0 noncon