FuncClass

GlycoEnzDB
Comprehensive glycoEnzyme database and dynamic pathway generation

Description: The GlycoEnzDB is a manually curated glycoEnzyme database, primarily focused on humans. It covers 390 enzymes across 28 pathway maps. Facilities are also available to create custom glycosylation reaction pathways using experimental data in SBML format and for pathway simulation
Contacts: Sriram Neelamegham (neel@buffalo.edu), Yusen Zhou (yusenzho@buffalo.edu) or Ted Groth (tgroth@buffalo.edu).

GlycoEnzDB
Create glycosylation pathway
Custom database generation
Instructions/Reference
 
Pathway Map
CHST3



General Information
Gene Symbol:   
CHST3
Protein name:   
Carbohydrate sulfotransferase 3
Enzyme Class:   
Sulfate synthase
CAZy Family:   
E.C. No:   
2.8.2.17
Organs:   
Reaction (SNFG):   
PAPS +
→
+ PAP
PAPS +
→
+ PAP
Reaction (IUBMB):   
No specified reaction.
About

• Chondroitin sulfate chains are composed of GlcAβ1-3GalNAcβ1-4 repeating unit. Various sulfotransferases, CHST3, 7, 11, 12, 13, 14, 15, and UST, sulfate either GalNAc and GlcA residues. CS are a major functional component of aggrecan, and needed for cartilage formation and maintenance. It is also involved in interactions with growth factors pleiotrophin and midkine.
• CHST3 (C6ST-1) and CHST7 (C6ST-2) are Chondroitin 6-sulfotransferases that attach sulfate to C6 of GalNAc. They are both type II transmembrane proteins.
• CHST3 can also sulfate C6 of Gal in keratin sulfate and sialyl LacNAc oligonucleotides, but not SLeX. It may also sulfate GAG protein linkage. This is not the only sulfoT capable of modifying keratin sulfate.
• C4-sulfation prevents C6-sulfation by CHST3.
• CHST3 KO-mice are fertile and show no obvious pathological features. Some repair mechanisms were impaired.
• CHST3 and CHST15 are secreted into culture medium.

Related Pathway
KEGG ORTHOLOGY:   
K01020
KEGG Pathway:   
ko00532 
Reactome:   
R-HSA-2022870
Related Database
Organism:   
Homo sapiens
Uniprot:   
Q7LGC8 
NCBI GeneID:   
9469
GlyMap GeneID:   
9469
DNA aRefSeq:   
NM_004273.4 
Protein RefSeq:   
NP_004264.2 
BRENDA:   
Kinetic info.
OMIM number:   
603799
GeneCards database:   
CHST3
Enzyme Specificity
Glycan Type:  
     N-linked, O-linked (mucin type), GAG
Compartment:  
     Cis, Medial, Trans, TGN
Substrate:  
     GlcA(b1-3)GalNAc(b1-4)GlcA(b1-3) or NeuAc(a2-3)Gal(b1-4)GlcNAc(b1-3 [6S])
Product:  
     GlcA(b1-3)GalNAc(b1-4 [6S])GlcA(b1-3) or NeuAc(a2-3)Gal(b1-4 [6S])GlcNAc(b1-3 [6S])
Constraints:  
     var=50%
Simulation Parameters
Mechaelis-Menten parameters (suggested)
VM:       (pmol/cell • hr)       KM:       (pM)
Relative conc of enzymes in compartment.(sum will be normalized to 1)
ER Cis- Medial- Trans- TGN










Expression Data in Tissue {ref. 5} Source from NCBI gene database
adrenal appendix bone marrow brain colon duodenum endometrium esophagus fat gall bladder heart kidney liver lung lymph node ovary pancreas placenta prostate salivary gland skin small intestine spleen stomach testis thyroid urinary bladder 20 10 0 RPKM
Expression Data in Cells {ref. 13} Source from Cancer Cell Line Encyclopedia database
Kidney Lung Breast Prostate Large intestine Ovary HL60 haematopoietic and lymphoid tissue Skin Central nervous system 786O A498 ACHN CAKI1 UO31 A549 EKVX HOP62 HOP92 NCIH226 NCIH23 NCIH322 NCIH460 NCIH522 SHP77 BT549 HS578T MCF7 MDAMB231 MDAMB468 T47D DU145 PC3 HCT116 HCT15 HT29 KM12 SW620 OVCAR4 OVCAR8 IGROV1 SKOV3 HL60 K562 RPMI8226 LOXIMVI MALME3M RPMI7951 SKMEL28 SKMEL5 UACC257 UACC62 SF268 SF295 SF539 SNB75 60 50 40 30 20 10 0 Transcripts per million (TPM)
Transcription factor {ref. 10}
Transcriptional start site (TSS) 0 200 -2000 -1800 -1600 -1400 -1200 -1000 -800 -600 -400 -200
ENST00000373115 TFAP2C REST NFIC SP1 ZFX MYC ZBTB26 ZNF600 GATA2 MAX MAZ ZBTB33 ZSCAN9 ZNF335 ZFP64 TEAD4 REST EGR3 EGR2 EGR2 MAZ SP3 KLF15 WT1 SP5 ZNF398 ZNF263 SP4 ZNF467 KLF9
miRNA-GlycoEnzyme Interactome {ref. 9}
miRNA Name Pos. Context Score Frac. Wt. Context Frac. Conserved Quality
hsa-miR-6820-5p 52-59 -0.483 95.0 -0.483 96.0 noncon
hsa-miR-657 5086-5093 -0.363 98.0 -0.007 44.0 noncon
hsa-miR-4290 3413-3420 -0.344 93.0 -0.333 94.0 noncon
hsa-miR-6801-3p 795-802 -0.343 96.0 -0.332 96.0 noncon
hsa-miR-3193 1298-1305 -0.33 92.0 -0.32 94.0 noncon
hsa-miR-1296-5p 1683-1690 -0.33 91.0 -0.32 94.0 noncon
hsa-miR-6798-5p 4637-4644 -0.328 96.0 -0.318 97.0 noncon
hsa-miR-4660 602-609 -0.327 96.0 -0.317 97.0 noncon
hsa-miR-15a-3p 5037-5044 -0.326 98.0 -0.316 99.0 noncon
hsa-miR-6810-3p 795-802 -0.325 95.0 -0.315 96.0 noncon
hsa-miR-6503-3p 4668-4675 -0.315 97.0 -0.305 98.0 noncon
hsa-miR-3173-5p 790-797 -0.303 95.0 -0.294 96.0 noncon
hsa-miR-6799-3p 790-797 -0.295 95.0 -0.286 96.0 noncon
hsa-miR-4529-5p 5036-5042 -0.29 92.0 -0.281 94.0 noncon
hsa-miR-128-1-5p 149-156 -0.277 93.0 -0.277 94.0 noncon
hsa-miR-1301-3p 603-610 -0.268 95.0 -0.26 96.0 noncon
hsa-miR-7160-3p 2801-2807 -0.264 93.0 -0.256 94.0 noncon
hsa-miR-550b-2-5p 670-677 -0.261 94.0 -0.253 96.0 noncon
hsa-miR-4529-3p 4670-4677 -0.257 91.0 -0.249 93.0 noncon
hsa-miR-6812-5p 2156-2163 -0.255 90.0 -0.247 91.0 noncon
hsa-miR-5047 603-610 -0.252 94.0 -0.244 96.0 noncon
hsa-miR-328-3p 1126-1133 -0.25 93.0 -0.242 95.0 noncon