FuncClass

GlycoEnzDB
Comprehensive glycoEnzyme database and dynamic pathway generation

Description: The GlycoEnzDB is a manually curated glycoEnzyme database, primarily focused on humans. It covers 390 enzymes across 28 pathway maps. Facilities are also available to create custom glycosylation reaction pathways using experimental data in SBML format and for pathway simulation
Contacts: Sriram Neelamegham (neel@buffalo.edu), Yusen Zhou (yusenzho@buffalo.edu) or Ted Groth (tgroth@buffalo.edu).

GlycoEnzDB
Create glycosylation pathway
Custom database generation
Instructions/Reference
 
Pathway Map
GNS



General Information
Gene Symbol:   
GNS
Protein name:   
N-acetylglucosamine-6-sulfatase
Enzyme Class:   
CAZy Family:   
E.C. No:   
3.1.6.14
Organs:   
Reaction (SNFG):   
→
Reaction (IUBMB):   
No specified reaction.
About

• Ubiquitously expressed lysosomal enzyme that promotes hydrolysis of the 6-sulfate groups of the GlcNAC6S units of heparan sulfate and keratan sulfate.
• This enzyme is deficient in Sanfilippo Syndrome type IIId.

Related Pathway
KEGG ORTHOLOGY:   
KEGG Pathway:   
No Record 
Reactome:   
R-HSA-2022857
Related Database
Organism:   
Homo sapiens
Uniprot:   
P15586 
NCBI GeneID:   
2799
GlyMap GeneID:   
2799
DNA aRefSeq:   
NM_002076.3 
Protein RefSeq:   
NP_002067.1 
BRENDA:   
Kinetic info.
OMIM number:   
607664
GeneCards database:   
GNS
Enzyme Specificity
Glycan Type:  
     
Compartment:  
     
Substrate:  
     GlcNAc(a1-4 S-6)
Product:  
     GlcNAc(a1-4)
Constraints:  
     None
Simulation Parameters
Mechaelis-Menten parameters (suggested)
VM:       (pmol/cell • hr)       KM:       (pM)
Relative conc of enzymes in compartment.(sum will be normalized to 1)
ER Cis- Medial- Trans- TGN










Expression Data in Tissue {ref. 5} Source from NCBI gene database
adrenal appendix bone marrow brain colon duodenum endometrium esophagus fat gall bladder heart kidney liver lung lymph node ovary pancreas placenta prostate salivary gland skin small intestine spleen stomach testis thyroid urinary bladder 100 80 60 40 20 0 RPKM
Expression Data in Cells {ref. 13} Source from Cancer Cell Line Encyclopedia database
Kidney Lung Breast Prostate Large intestine Ovary HL60 haematopoietic and lymphoid tissue Skin Central nervous system 786O A498 ACHN CAKI1 UO31 A549 EKVX HOP62 HOP92 NCIH226 NCIH23 NCIH322 NCIH460 NCIH522 SHP77 BT549 HS578T MCF7 MDAMB231 MDAMB468 T47D DU145 PC3 HCT116 HCT15 HT29 KM12 SW620 OVCAR4 OVCAR8 IGROV1 SKOV3 HL60 K562 RPMI8226 LOXIMVI MALME3M RPMI7951 SKMEL28 SKMEL5 UACC257 UACC62 SF268 SF295 SF539 SNB75 200 180 160 140 120 100 80 60 40 20 0 Transcripts per million (TPM)
Transcription factor {ref. 10}
No high degree of confidence data
miRNA-GlycoEnzyme Interactome {ref. 9}
miRNA Name Pos. Context Score Frac. Wt. Context Frac. Conserved Quality
hsa-miR-3156-3p 3292-3299 -0.587 99.0 0.0 0.0 noncon
hsa-miR-1268b 1360-1367 -0.483 90.0 -0.472 93.0 noncon
hsa-miR-1268a 1360-1367 -0.483 90.0 -0.472 93.0 noncon
hsa-miR-4301 3291-3298 -0.462 99.0 -0.132 89.0 noncon
hsa-miR-4773 3285-3292 -0.409 99.0 -0.34 99.0 noncon
hsa-miR-1294 1776-1783 -0.409 98.0 -0.4 98.0 noncon
hsa-miR-4731-5p 1787-1794 -0.382 98.0 -0.373 98.0 noncon
hsa-miR-874-5p 279-286 -0.365 96.0 -0.361 97.0 noncon
hsa-miR-585-5p 2259-2266 -0.342 96.0 -0.287 95.0 noncon
hsa-miR-27a-3p 175-182 -0.339 98.0 -0.338 98.0 con
hsa-miR-27b-3p 175-182 -0.339 98.0 -0.338 98.0 con
hsa-miR-194-3p 3139-3146 -0.338 98.0 -0.282 97.0 noncon
hsa-miR-4471 3135-3142 -0.325 95.0 -0.272 95.0 noncon
hsa-miR-6755-3p 2909-2916 -0.303 93.0 -0.254 92.0 noncon
hsa-miR-6857-3p 3206-3213 -0.3 96.0 -0.251 96.0 noncon
hsa-miR-6801-3p 1595-1602 -0.299 94.0 -0.292 95.0 noncon
hsa-miR-6861-3p 2419-2426 -0.288 92.0 -0.241 92.0 noncon
hsa-miR-3934-3p 2058-2065 -0.28 95.0 -0.236 95.0 noncon
hsa-miR-3165 3080-3087 -0.276 96.0 -0.231 95.0 noncon
hsa-miR-3944-5p 3254-3260 -0.273 95.0 -0.229 95.0 noncon
hsa-miR-6856-3p 919-926 -0.267 94.0 -0.261 96.0 noncon
hsa-miR-29a-3p 2585-2591 -0.265 90.0 -0.222 90.0 con
hsa-miR-29b-3p 2585-2591 -0.265 90.0 -0.222 90.0 con
hsa-miR-29c-3p 2585-2591 -0.265 90.0 -0.222 90.0 con
hsa-miR-200c-5p 1694-1701 -0.265 95.0 -0.259 96.0 noncon
hsa-miR-8059 3135-3142 -0.265 95.0 -0.222 95.0 noncon
hsa-miR-6735-5p 3186-3192 -0.264 94.0 -0.222 93.0 noncon
hsa-miR-3124-3p 937-944 -0.256 96.0 -0.25 97.0 noncon
hsa-miR-106b-5p 3257-3263 -0.255 97.0 -0.214 96.0 con
hsa-miR-6079 2217-2224 -0.255 96.0 -0.215 95.0 noncon
hsa-miR-631 2012-2018 -0.254 90.0 -0.215 90.0 noncon
hsa-miR-3157-5p 1702-1709 -0.253 95.0 -0.248 96.0 noncon
hsa-miR-597-3p 2447-2454 -0.253 93.0 -0.213 93.0 noncon
hsa-miR-4695-5p 2367-2373 -0.25 94.0 -0.21 94.0 noncon
hsa-miR-5579-5p 520-526 -0.25 94.0 -0.245 96.0 noncon