FuncClass

GlycoEnzDB
Comprehensive glycoEnzyme database and dynamic pathway generation

Description: The GlycoEnzDB is a manually curated glycoEnzyme database, primarily focused on humans. It covers 390 enzymes across 28 pathway maps. Facilities are also available to create custom glycosylation reaction pathways using experimental data in SBML format and for pathway simulation
Contacts: Sriram Neelamegham (neel@buffalo.edu), Yusen Zhou (yusenzho@buffalo.edu) or Ted Groth (tgroth@buffalo.edu).

GlycoEnzDB
Create glycosylation pathway
Custom database generation
Instructions/Reference
 
Pathway Map
HS2ST1



General Information
Gene Symbol:   
HS2ST1
Protein name:   
Heparan sulfate 2-O-sulfotransferase 1
Enzyme Class:   
Sulfate synthase
CAZy Family:   
E.C. No:   
2.8.2.*
Organs:   
Reaction (SNFG):   
PAPS +
→
+ PAP
PAPS +
→
+ PAP
Reaction (IUBMB):   
No specified reaction.
About

• Sulfation of heparan sulfate occurs at C2 of HexA. It also occurs at C3, C6, and NS residues of GlcNAc or GlcNS. HSPGs are major regulators of cell behavior with roles in inflammation, blood coagulation, tumor invasion, and malignancy.
• 2-O-sulfation in HS/heparin plays pivotal roles in regulating biological function.
• HS2ST is closely related to UST. It transfers sulfo group to C2 of GlcA or IdoA in the context of N-sulfated GlcNAc, but not 6-sulfated GlcNAc. Thus 2-O-sulfation precedes 6-O-sulfation of GlcNSO3.
• HS2ST1 forms a complex with GLCE to facilitate conversion of GlcA to IdoA2S.
• HS2ST-deficient mice died in the neonatal period. Other defects are also associated with HS2ST function.
• CS, DS and KS are not acceptors for HS2ST activity.

Related Pathway
KEGG ORTHOLOGY:   
K02513
KEGG Pathway:   
ko00534 
Reactome:   
R-HSA-2022928
Related Database
Organism:   
Homo sapiens
Uniprot:   
Q7LGA3 
NCBI GeneID:   
9653
GlyMap GeneID:   
9653
DNA aRefSeq:   
NM_001134492.1 
Protein RefSeq:   
NP_001127964.1 
BRENDA:   
Kinetic info.
OMIM number:   
604844
GeneCards database:   
HS2ST1
Enzyme Specificity
Glycan Type:  
     GAG
Compartment:  
     Cis, Medial, Trans, TGN
Substrate:  
     GlcN(a1-4 [NS])GlcA(b1-4) or GlcN(a1-4 [NS])IdoA(a1-3)
Product:  
     GlcN(a1-4 [NS])GlcA(b1-4 [2S]) or GlcN(a1-4 [NS])IdoA(a1-3 [2S])
Constraints:  
     ! <[6S]> or !<[2,6S]> and var=50%
Simulation Parameters
Mechaelis-Menten parameters (suggested)
VM:       (pmol/cell • hr)       KM:       (pM)
Relative conc of enzymes in compartment.(sum will be normalized to 1)
ER Cis- Medial- Trans- TGN










Expression Data in Tissue {ref. 5} Source from NCBI gene database
adrenal appendix bone marrow brain colon duodenum endometrium esophagus fat gall bladder heart kidney liver lung lymph node ovary pancreas placenta prostate salivary gland skin small intestine spleen stomach testis thyroid urinary bladder 20 10 0 RPKM
Expression Data in Cells {ref. 13} Source from Cancer Cell Line Encyclopedia database
Kidney Lung Breast Prostate Large intestine Ovary HL60 haematopoietic and lymphoid tissue Skin Central nervous system 786O A498 ACHN CAKI1 UO31 A549 EKVX HOP62 HOP92 NCIH226 NCIH23 NCIH322 NCIH460 NCIH522 SHP77 BT549 HS578T MCF7 MDAMB231 MDAMB468 T47D DU145 PC3 HCT116 HCT15 HT29 KM12 SW620 OVCAR4 OVCAR8 IGROV1 SKOV3 HL60 K562 RPMI8226 LOXIMVI MALME3M RPMI7951 SKMEL28 SKMEL5 UACC257 UACC62 SF268 SF295 SF539 SNB75 40 30 20 10 0 Transcripts per million (TPM)
Transcription factor {ref. 10}
No high degree of confidence data
miRNA-GlycoEnzyme Interactome {ref. 9}
miRNA Name Pos. Context Score Frac. Wt. Context Frac. Conserved Quality
hsa-miR-6087 5325-5332 -0.567 99.0 -0.002 17.0 noncon
hsa-miR-6729-5p 2059-2066 -0.528 97.0 -0.456 96.0 noncon
hsa-miR-4649-5p 2059-2066 -0.504 96.0 -0.436 95.0 noncon
hsa-miR-129-1-3p 4765-4772 -0.484 99.0 -0.186 93.0 noncon
hsa-miR-129-2-3p 4765-4772 -0.484 99.0 -0.186 93.0 noncon
hsa-miR-133b 983-990 -0.433 97.0 -0.423 97.0 con
hsa-miR-133a-3p.2 983-990 -0.433 97.0 -0.423 97.0 con
hsa-miR-28-5p 2512-2519 -0.394 98.0 -0.343 98.0 con
hsa-miR-3139 2512-2519 -0.392 98.0 -0.341 98.0 con
hsa-miR-708-5p 2512-2519 -0.386 98.0 -0.336 98.0 con
hsa-miR-222-5p 419-426 -0.378 98.0 -0.378 99.0 noncon
hsa-miR-4659b-3p 4747-4754 -0.358 98.0 -0.142 91.0 noncon
hsa-miR-4659a-3p 4747-4754 -0.358 98.0 -0.142 92.0 noncon
hsa-miR-548v 5342-5349 -0.356 97.0 -0.002 21.0 noncon
hsa-miR-1301-5p 2188-2195 -0.355 97.0 -0.31 97.0 noncon
hsa-miR-492 2722-2729 -0.343 95.0 -0.299 95.0 noncon
hsa-miR-6502-5p 2188-2195 -0.336 96.0 -0.293 96.0 noncon
hsa-miR-1251-5p 3503-3510 -0.335 97.0 -0.15 91.0 noncon
hsa-miR-449b-5p 3885-3892 -0.335 93.0 -0.149 77.0 con
hsa-miR-124-3p.1 4236-4242 -0.328 96.0 -0.146 90.0 con
hsa-miR-4763-5p 1970-1976 -0.328 91.0 -0.286 93.0 noncon
hsa-miR-34c-5p 3885-3892 -0.327 93.0 -0.145 76.0 con
hsa-miR-634 4697-4704 -0.324 97.0 -0.139 88.0 noncon
hsa-miR-6800-5p 255-262 -0.317 97.0 -0.317 98.0 noncon
hsa-miR-449a 3885-3892 -0.317 92.0 -0.141 77.0 con
hsa-miR-3684 277-284 -0.316 94.0 -0.316 96.0 noncon
hsa-miR-4716-3p 4875-4881 -0.313 92.0 -0.125 75.0 noncon
hsa-miR-4763-3p 2302-2309 -0.312 96.0 -0.273 95.0 noncon
hsa-miR-4715-5p 3826-3833 -0.312 95.0 -0.139 88.0 noncon
hsa-miR-518b 2840-2846 -0.309 92.0 -0.27 92.0 noncon
hsa-miR-518d-3p 2840-2846 -0.309 92.0 -0.27 92.0 noncon
hsa-miR-518c-3p 2840-2846 -0.309 91.0 -0.27 92.0 noncon
hsa-miR-3651 4767-4773 -0.308 94.0 -0.123 82.0 noncon
hsa-miR-656-5p 2416-2423 -0.303 92.0 -0.265 93.0 noncon
hsa-miR-34a-5p 3885-3892 -0.301 91.0 -0.134 75.0 con
hsa-miR-3171 250-257 -0.301 95.0 -0.301 97.0 noncon
hsa-miR-4790-3p 1438-1445 -0.298 97.0 -0.292 98.0 noncon
hsa-miR-4659a-3p 2975-2982 -0.298 97.0 -0.26 97.0 noncon
hsa-miR-4659b-3p 2975-2982 -0.298 97.0 -0.26 97.0 noncon
hsa-miR-134-3p 2561-2568 -0.297 96.0 -0.26 96.0 noncon
hsa-miR-593-5p 3376-3383 -0.296 92.0 -0.151 80.0 noncon
hsa-miR-4705 3058-3064 -0.291 97.0 -0.254 97.0 noncon
hsa-miR-383-5p.1 3296-3302 -0.289 94.0 -0.148 82.0 noncon
hsa-miR-6889-3p 4816-4822 -0.285 95.0 -0.114 84.0 noncon
hsa-miR-4527 2406-2413 -0.282 94.0 -0.247 94.0 noncon
hsa-miR-3913-3p 5096-5103 -0.28 94.0 -0.112 75.0 noncon
hsa-miR-1233-5p 2559-2566 -0.278 92.0 -0.243 92.0 noncon
hsa-miR-6503-5p 2406-2413 -0.276 94.0 -0.242 94.0 noncon
hsa-miR-3612 658-664 -0.275 95.0 -0.269 96.0 noncon
hsa-miR-6756-3p 4478-4485 -0.273 92.0 -0.118 81.0 noncon
hsa-miR-4711-5p 4448-4455 -0.272 94.0 -0.122 82.0 noncon
hsa-miR-3613-3p 5287-5293 -0.272 99.0 -0.089 95.0 noncon
hsa-miR-1207-5p 2302-2309 -0.27 93.0 -0.236 93.0 noncon
hsa-miR-215-3p 1907-1914 -0.266 97.0 -0.233 97.0 noncon
hsa-miR-6509-3p 1170-1177 -0.264 95.0 -0.258 96.0 noncon
hsa-miR-596 1971-1977 -0.262 97.0 -0.229 97.0 noncon
hsa-miR-6778-5p 2559-2566 -0.262 90.0 -0.229 90.0 noncon
hsa-miR-518f-3p 2840-2846 -0.261 92.0 -0.228 92.0 noncon
hsa-miR-518a-3p 2840-2846 -0.261 92.0 -0.228 92.0 noncon
hsa-miR-6776-3p 557-563 -0.257 95.0 -0.257 97.0 noncon
hsa-miR-182-3p 2774-2781 -0.256 95.0 -0.224 95.0 noncon
hsa-miR-1178-5p 38-44 -0.253 92.0 -0.253 94.0 noncon
hsa-miR-3194-5p 158-165 -0.253 93.0 -0.253 95.0 noncon
hsa-miR-203a-5p 2776-2782 -0.251 92.0 -0.22 93.0 noncon
hsa-miR-6871-3p 3218-3224 -0.251 94.0 -0.129 86.0 noncon