FuncClass

GlycoEnzDB
Comprehensive glycoEnzyme database and dynamic pathway generation

Description: The GlycoEnzDB is a manually curated glycoEnzyme database, primarily focused on humans. It covers 390 enzymes across 28 pathway maps. Facilities are also available to create custom glycosylation reaction pathways using experimental data in SBML format and for pathway simulation
Contacts: Sriram Neelamegham (neel@buffalo.edu), Yusen Zhou (yusenzho@buffalo.edu) or Ted Groth (tgroth@buffalo.edu).

GlycoEnzDB
Create glycosylation pathway
Custom database generation
Instructions/Reference
 
Pathway Map
HS3ST6



General Information
Gene Symbol:   
HS3ST6
Protein name:   
Heparan sulfate glucosamine 3-O-sulfotransferase 6
Enzyme Class:   
Sulfate synthase
CAZy Family:   
E.C. No:   
2.8.2.23
Organs:   
Reaction (SNFG):   
PAPS +
→
+ PAP
PAPS +
→
+ PAP
Reaction (IUBMB):   
No specified reaction.
About

• Sulfation of heparan sulfate occurs at C2 of HexA. It also occurs at C3, C6, and NS residues of GlcNAc or GlcNS. HSPGs are major regulators of cell behavior with roles in inflammation, blood coagulation, tumor invasion, and malignancy.
• Seven isoenzymes display HS 3-O-sulfotransferase activity, with all enzymes transferring sulfate to C3 of GlcNAc in heparin and heparan sulfate.
• HS3ST1 sulfates an N-sulfo glucosamine (GlcNS) that has a nonsulfated glucuronic acid (GlcA) or an iduronic acid (IdoA) at the nonreducing end. This modification is necessary for anti-thrombin activity of heparin. HS
• HS3ST-2, 3 (3A and 3B), 4, and 6, all sulfate a GlcNS6S residue that has a 2-O-sulfated iduronic acid (IdoA2S) on the nonreducing end. This does not provide anti-thrombin activity, but serves viral entry for herpes simplex virus type-1.
• The tissue distribution of the different HS3STs is quite different.

Related Pathway
KEGG ORTHOLOGY:   
K09679
KEGG Pathway:   
 
Reactome:   
R-HSA-2022928
Related Database
Organism:   
Homo sapiens
Uniprot:   
Q96QI5 
NCBI GeneID:   
64711
GlyMap GeneID:   
64711
DNA aRefSeq:   
NM_001009606.3 
Protein RefSeq:   
NP_001009606.3 
BRENDA:   
Kinetic info.
OMIM number:   
-
GeneCards database:   
HS3ST6
Enzyme Specificity
Glycan Type:  
     GAG
Compartment:  
     Cis, Medial, Trans, TGN
Substrate:  
     IdoA(a1-4 [2S])GlcN(a1-4 [2S]) or IdoA(a1-4 [2S])GlcN(a1-4 [2,6S])
Product:  
     IdoA(a1-4 [2S])GlcN(a1-4 [2,3S]) or IdoA(a1-4 [2S])GlcN(a1-4 [2,3,6S])
Simulation Parameters
Mechaelis-Menten parameters (suggested)
VM:       (pmol/cell • hr)       KM:       (pM)
Relative conc of enzymes in compartment.(sum will be normalized to 1)
ER Cis- Medial- Trans- TGN










Expression Data in Tissue {ref. 5} Source from NCBI gene database
adrenal appendix bone marrow brain colon duodenum endometrium esophagus fat gall bladder heart kidney liver lung lymph node ovary pancreas placenta prostate salivary gland skin small intestine spleen stomach testis thyroid urinary bladder 4 2 0 RPKM
Expression Data in Cells {ref. 13} Source from Cancer Cell Line Encyclopedia database
Kidney Lung Breast Prostate Large intestine Ovary HL60 haematopoietic and lymphoid tissue Skin Central nervous system 786O A498 ACHN CAKI1 UO31 A549 EKVX HOP62 HOP92 NCIH226 NCIH23 NCIH322 NCIH460 NCIH522 SHP77 BT549 HS578T MCF7 MDAMB231 MDAMB468 T47D DU145 PC3 HCT116 HCT15 HT29 KM12 SW620 OVCAR4 OVCAR8 IGROV1 SKOV3 HL60 K562 RPMI8226 LOXIMVI MALME3M RPMI7951 SKMEL28 SKMEL5 UACC257 UACC62 SF268 SF295 SF539 SNB75 4 2 0 Transcripts per million (TPM)
Transcription factor {ref. 10}
Transcriptional start site (TSS) 0 200 -2000 -1800 -1600 -1400 -1200 -1000 -800 -600 -400 -200
ENST00000293937 ENST00000454677 RELA EBF1 MYCN ASCL1 MAZ E2F4 RCOR1 TWIST1 SP1 MYCN ERG GABPA RUNX1 SP2 SP1 SP1 RUNX1 ERG MYC SP1 MAX KLF15 SP1 USF2 EGR2 RBPJ EGR3 RELA
miRNA-GlycoEnzyme Interactome {ref. 9}
miRNA Name Pos. Context Score Frac. Wt. Context Frac. Conserved Quality
hsa-miR-6833-5p 119-126 -0.764 99.0 -0.278 98.0 noncon
hsa-miR-4663 17-24 -0.606 99.0 -0.606 99.0 noncon
hsa-miR-7111-3p 80-87 -0.579 99.0 -0.579 99.0 noncon
hsa-miR-4761-5p 23-30 -0.551 99.0 -0.551 99.0 noncon
hsa-miR-6769b-3p 79-86 -0.533 99.0 -0.533 99.0 noncon
hsa-miR-4723-3p 79-86 -0.517 99.0 -0.517 99.0 noncon
hsa-miR-3183 79-86 -0.428 99.0 -0.428 99.0 noncon
hsa-miR-1298-3p 110-116 -0.415 98.0 -0.162 88.0 noncon
hsa-miR-3148 116-123 -0.41 99.0 -0.161 97.0 noncon
hsa-miR-3656 35-41 -0.383 95.0 -0.383 96.0 noncon
hsa-miR-18a-5p 22-28 -0.368 98.0 -0.368 98.0 noncon
hsa-miR-18b-5p 22-28 -0.368 98.0 -0.368 98.0 noncon
hsa-miR-6861-5p 109-115 -0.363 98.0 -0.363 99.0 noncon
hsa-miR-4735-3p 22-28 -0.358 97.0 -0.358 98.0 noncon
hsa-miR-4752 120-126 -0.347 98.0 -0.138 89.0 noncon
hsa-miR-3190-5p 47-54 -0.314 98.0 -0.314 98.0 noncon
hsa-miR-3190-3p 119-125 -0.308 96.0 -0.123 83.0 noncon
hsa-miR-6776-5p 109-115 -0.255 95.0 -0.255 96.0 noncon
hsa-miR-18a-3p 75-81 -0.252 95.0 -0.252 96.0 noncon