GlyClass

GlycoEnzDB
Comprehensive glycoEnzyme database and dynamic pathway generation

Description: The GlycoEnzDB is a manually curated glycoEnzyme database, primarily focused on humans. It covers 390 enzymes across 28 pathway maps. Facilities are also available to create custom glycosylation reaction pathways using experimental data in SBML format and for pathway simulation
Contacts: Sriram Neelamegham (neel@buffalo.edu), Yusen Zhou (yusenzho@buffalo.edu) or Ted Groth (tgroth@buffalo.edu).

GlycoEnzDB
Create glycosylation pathway
Custom database generation
Instructions/Reference
 
Pathway Map
CHST11



General Information
Gene Symbol:   
CHST11
Protein name:   
Carbohydrate sulfotransferase 11
Enzyme Class:   
Sulfate synthase
CAZy Family:   
E.C. No:   
2.8.2.5
Organs:   
Reaction (SNFG):   
PAPS +
→
+ PAP
Reaction (IUBMB):   
No specified reaction.
About

• Chondroitin sulfate chains are composed of GlcAβ1-3GalNAcβ1-4 repeating unit. Various sulfotransferases, CHST3, 7, 11-13, 14, 15, and UST, sulfate either GalNAc and GlcA residues. CS are a major functional component of aggrecan, and needed for cartilage formation and maintenance.
• CHST11-13 are three isoforms of the C4ST family, C4ST-1, C4ST-2 and C4ST-3.
• These enzymes transfer sulfate from PAPS donor to C4 of GalNAc on CS chains.
• CHST11 transfer sulfate to GalNAc residue adjacent to the reducing side of the GlcA, and also at the non-reducing end. Chondroitin 4-sulfate formed by C4STs serve as acceptors for GalNAc4S-6ST to form chondroitin sulfate E.
• CHST12 acts on GalNAc adjacent not only to GlcA but also IdoA in desulfated dermatan sulfate. The activity for the latter is lower.
• CHST13 displays similar activity to CHST11

Related Pathway
KEGG ORTHOLOGY:   
K01017
KEGG Pathway:   
ko00532 
Reactome:   
R-HSA-2022870
Related Database
Organism:   
Homo sapiens
Uniprot:   
Q9NPF2 
NCBI GeneID:   
50515
GlyMap GeneID:   
50515
DNA aRefSeq:   
NM_001173982.1 
Protein RefSeq:   
NP_001167453.1 
BRENDA:   
Kinetic info.
OMIM number:   
610128
GeneCards database:   
CHST11
Enzyme Specificity
Glycan Type:  
     GAG
Compartment:  
     Cis, Medial, Trans, TGN
Substrate:  
     GlcA(b1-3)GalNAc(b1-4)GlcA(b1-3)
Product:  
     GlcA(b1-3)GalNAc(b1-4 [4S])GlcA(b1-3)
Constraints:  
     var=50%
Simulation Parameters
Mechaelis-Menten parameters (suggested)
VM:       (pmol/cell • hr)       KM:       (pM)
Relative conc of enzymes in compartment.(sum will be normalized to 1)
ER Cis- Medial- Trans- TGN










Expression Data in Tissue {ref. 5} Source from NCBI gene database
adrenal appendix bone marrow brain colon duodenum endometrium esophagus fat gall bladder heart kidney liver lung lymph node ovary pancreas placenta prostate salivary gland skin small intestine spleen stomach testis thyroid urinary bladder 10 8 6 4 2 0 RPKM
Expression Data in Cells {ref. 13} Source from Cancer Cell Line Encyclopedia database
Kidney Lung Breast Prostate Large intestine Ovary HL60 haematopoietic and lymphoid tissue Skin Central nervous system 786O A498 ACHN CAKI1 UO31 A549 EKVX HOP62 HOP92 NCIH226 NCIH23 NCIH322 NCIH460 NCIH522 SHP77 BT549 HS578T MCF7 MDAMB231 MDAMB468 T47D DU145 PC3 HCT116 HCT15 HT29 KM12 SW620 OVCAR4 OVCAR8 IGROV1 SKOV3 HL60 K562 RPMI8226 LOXIMVI MALME3M RPMI7951 SKMEL28 SKMEL5 UACC257 UACC62 SF268 SF295 SF539 SNB75 100 80 60 40 20 0 Transcripts per million (TPM)
Transcription factor {ref. 10}
No high degree of confidence data
miRNA-GlycoEnzyme Interactome {ref. 9}
miRNA Name Pos. Context Score Frac. Wt. Context Frac. Conserved Quality
hsa-miR-6763-3p 1843-1850 -0.511 97.0 -0.421 97.0 noncon
hsa-miR-6801-3p 173-180 -0.493 98.0 -0.493 99.0 noncon
hsa-miR-6729-3p 104-111 -0.484 99.0 -0.484 99.0 noncon
hsa-miR-132-5p 2550-2557 -0.472 96.0 -0.389 95.0 noncon
hsa-miR-6810-3p 173-180 -0.467 98.0 -0.467 99.0 noncon
hsa-miR-3918 777-784 -0.396 98.0 -0.328 97.0 noncon
hsa-miR-4786-5p 314-321 -0.395 98.0 -0.327 98.0 noncon
hsa-miR-551a 188-194 -0.38 95.0 -0.38 97.0 noncon
hsa-miR-4524a-3p 225-232 -0.38 98.0 -0.38 99.0 noncon
hsa-miR-6508-3p 961-968 -0.376 98.0 -0.312 97.0 noncon
hsa-miR-4445-3p 1715-1721 -0.373 95.0 -0.31 94.0 noncon
hsa-miR-551b-3p 188-194 -0.369 94.0 -0.369 96.0 noncon
hsa-miR-6889-5p 2941-2947 -0.362 90.0 -0.301 86.0 noncon
hsa-miR-652-3p 3708-3714 -0.356 94.0 -0.296 93.0 noncon
hsa-miR-1299 357-364 -0.337 98.0 -0.28 97.0 noncon
hsa-miR-6738-5p 1334-1341 -0.337 97.0 -0.28 96.0 noncon
hsa-miR-5194 1334-1341 -0.324 97.0 -0.27 96.0 noncon
hsa-miR-3652 1551-1558 -0.315 98.0 -0.262 97.0 noncon
hsa-miR-6807-3p 4035-4042 -0.312 98.0 -0.26 98.0 con
hsa-miR-135b-3p 939-946 -0.309 92.0 -0.257 91.0 noncon
hsa-miR-138-5p 3799-3806 -0.309 93.0 -0.257 92.0 noncon
hsa-miR-3189-3p 613-620 -0.309 96.0 -0.257 96.0 noncon
hsa-miR-101-3p.1 4080-4086 -0.308 96.0 -0.257 96.0 con
hsa-miR-217 4035-4042 -0.299 98.0 -0.249 98.0 con
hsa-miR-4430 1551-1558 -0.288 97.0 -0.24 97.0 noncon
hsa-miR-591 312-318 -0.286 93.0 -0.239 92.0 noncon
hsa-miR-135b-5p 4153-4159 -0.286 94.0 -0.239 94.0 con
hsa-miR-135a-5p 4153-4159 -0.286 94.0 -0.239 94.0 con
hsa-miR-6734-3p 154-161 -0.279 95.0 -0.279 96.0 noncon
hsa-miR-450a-2-3p 2281-2288 -0.278 92.0 -0.232 91.0 noncon
hsa-miR-3675-5p 1474-1480 -0.277 93.0 -0.231 93.0 noncon
hsa-miR-1294 978-985 -0.277 95.0 -0.231 94.0 noncon
hsa-miR-5587-5p 3729-3736 -0.275 95.0 -0.23 95.0 noncon
hsa-miR-654-5p 3748-3754 -0.27 93.0 -0.226 93.0 noncon
hsa-miR-367-5p 2500-2507 -0.265 94.0 -0.221 94.0 noncon
hsa-miR-548v 2303-2310 -0.264 92.0 -0.221 92.0 noncon
hsa-miR-1914-3p 1334-1341 -0.264 97.0 -0.221 96.0 noncon
hsa-miR-766-5p 3808-3815 -0.264 95.0 -0.221 94.0 noncon
hsa-miR-3127-5p 778-785 -0.262 92.0 -0.219 92.0 noncon
hsa-miR-1226-5p 817-824 -0.262 95.0 -0.219 94.0 noncon
hsa-miR-6850-3p 1880-1886 -0.261 90.0 -0.218 89.0 noncon
hsa-miR-7158-5p 755-762 -0.261 97.0 -0.218 97.0 noncon
hsa-miR-541-3p 3748-3754 -0.259 93.0 -0.216 92.0 noncon
hsa-miR-5571-5p 130-137 -0.256 99.0 -0.256 99.0 noncon
hsa-miR-6802-3p 1731-1737 -0.255 90.0 -0.213 91.0 noncon
hsa-miR-3160-3p 394-400 -0.253 95.0 -0.212 94.0 noncon
hsa-miR-6069 3644-3651 -0.251 91.0 -0.21 90.0 noncon