GlyClass

GlycoEnzDB
Comprehensive glycoEnzyme database and dynamic pathway generation

Description: The GlycoEnzDB is a manually curated glycoEnzyme database, primarily focused on humans. It covers 390 enzymes across 28 pathway maps. Facilities are also available to create custom glycosylation reaction pathways using experimental data in SBML format and for pathway simulation
Contacts: Sriram Neelamegham (neel@buffalo.edu), Yusen Zhou (yusenzho@buffalo.edu) or Ted Groth (tgroth@buffalo.edu).

GlycoEnzDB
Create glycosylation pathway
Custom database generation
Instructions/Reference
 
Pathway Map
CSGALNACT1



General Information
Gene Symbol:   
CSGALNACT1
Protein name:   
Chondroitin sulfate N-acetylgalactosaminyltransferase 1
Enzyme Class:   
Glycosyltransferase
CAZy Family:   
GT7
E.C. No:   
2.4.1.174
Organs:   
Reaction (SNFG):   
+
→
+ UDP
+
→
+ UDP
Reaction (IUBMB):   
No specified reaction.
About

• The GalNAcT-I activity catalyzes GalNAc transfer toward GlcA in the linkage tetrasaccharide (GlcAβ1-3Galβ1-3Galβ1-4Xylβ1-). This is strongly mediated by CSGalNAcT1 (ChGn1) but can also be performed by CSGalNAcT-II (ChGn2).
• GalNAcT-II catalyzes elongation activity to create repeating disaccharide unit (GlcAβ1-3GalNAcβ1-4)n in the CS chain. This is more readily performed by CSGalNAcT2. Mice lacking CSGalNAcT1 exhibit dwarf phenotype with less cartilage development
• Overall, CSGalNAcT1 and CSGalNAcT2 mediate both initiation and elongation of CS via GalNAcT activity
• CHSY1 (Chondroitin synthase-1/chondroitin sulfate synthase-1 (CSS1)) and CHSY3 (chondroitin synthase-3/chondroitin sulfate synthase-3 (CSS3)) exhibit dual glycosyltransferase activities of β1,3 GlcA-T and β1,4 GalNAc-T for CS initiation and elongation
• CHPF (chondroitin sulfate synthase-2 (CSS2)) also exhibits dual glycosyltransferase activities of β1,3 GlcA-T and β1,4 GalNAc-T for CS initiation and elongation. Its activity is similar to that of CSS1 and CSS3.
• CHPF2 (chondroitin polymerizing factor 2/chondroitin sulfate glucuronyltransferase (CSGlcA-T)) transfers glucuronic acid (GlcUA) from UDP-GlcUA to N-acetylgalactosamine in the elongating chondroitin polymer. It does not have GalNAcT activity.
• CS is also present broadly in the tissues and abundantly in the cartilage and brain. Aggrecan contains abundant CS in cartilage.

Related Pathway
KEGG ORTHOLOGY:   
K00746
KEGG Pathway:   
ko00532  ko01100 
Reactome:   
R-HSA-2022870
Related Database
Organism:   
Homo sapiens
Uniprot:   
Q8TDX6 
NCBI GeneID:   
55790
GlyMap GeneID:   
55790
DNA aRefSeq:   
NM_001130518.1 
Protein RefSeq:   
NP_001123990.1 
BRENDA:   
Kinetic info.
OMIM number:   
616615
GeneCards database:   
CSGALNACT1
Enzyme Specificity
Glycan Type:  
     GAG
Compartment:  
     Cis, Medial, Trans, TGN
Substrate:  
     GlcA(b1-3)Gal(b1-3)Gal(b1-4)Xyl(b1-?)Ser or GlcA(b1-3)GalNAc(b1-4)
Product:  
     GalNAc(b1-4)GlcA(b1-3)Gal(b1-3)Gal(b1-4)Xyl(b1-?)Ser or GalNAc(b1-4)GlcA(b1-3)GalNAc(b1-4)
Simulation Parameters
Mechaelis-Menten parameters (suggested)
VM:       (pmol/cell • hr)       KM:       (pM)
Relative conc of enzymes in compartment.(sum will be normalized to 1)
ER Cis- Medial- Trans- TGN










Expression Data in Tissue {ref. 5} Source from NCBI gene database
adrenal appendix bone marrow brain colon duodenum endometrium esophagus fat gall bladder heart kidney liver lung lymph node ovary pancreas placenta prostate salivary gland skin small intestine spleen stomach testis thyroid urinary bladder 30 20 10 0 RPKM
Expression Data in Cells {ref. 13} Source from Cancer Cell Line Encyclopedia database
Kidney Lung Breast Prostate Large intestine Ovary HL60 haematopoietic and lymphoid tissue Skin Central nervous system 786O A498 ACHN CAKI1 UO31 A549 EKVX HOP62 HOP92 NCIH226 NCIH23 NCIH322 NCIH460 NCIH522 SHP77 BT549 HS578T MCF7 MDAMB231 MDAMB468 T47D DU145 PC3 HCT116 HCT15 HT29 KM12 SW620 OVCAR4 OVCAR8 IGROV1 SKOV3 HL60 K562 RPMI8226 LOXIMVI MALME3M RPMI7951 SKMEL28 SKMEL5 UACC257 UACC62 SF268 SF295 SF539 SNB75 80 60 40 20 0 Transcripts per million (TPM)
Transcription factor {ref. 10}
No high degree of confidence data
miRNA-GlycoEnzyme Interactome {ref. 9}
miRNA Name Pos. Context Score Frac. Wt. Context Frac. Conserved Quality
hsa-miR-4268 910-917 -0.524 99.0 -0.524 99.0 noncon
hsa-miR-7152-3p 504-511 -0.488 99.0 -0.488 99.0 noncon
hsa-miR-6734-3p 848-855 -0.482 99.0 -0.482 99.0 noncon
hsa-miR-5580-5p 1398-1405 -0.458 98.0 -0.458 98.0 noncon
hsa-miR-5091 600-606 -0.457 96.0 -0.457 97.0 noncon
hsa-miR-136-5p 1144-1151 -0.455 99.0 -0.455 99.0 noncon
hsa-miR-7156-3p 59-66 -0.447 99.0 -0.447 99.0 noncon
hsa-miR-6511b-5p 184-191 -0.438 98.0 -0.438 99.0 noncon
hsa-miR-6811-5p 184-191 -0.419 98.0 -0.419 99.0 noncon
hsa-miR-7846-3p 175-181 -0.414 95.0 -0.414 96.0 noncon
hsa-miR-199a-5p 923-930 -0.41 97.0 -0.41 98.0 con
hsa-miR-199b-5p 923-930 -0.41 97.0 -0.41 98.0 con
hsa-miR-6735-3p 1561-1568 -0.398 98.0 -0.398 99.0 noncon
hsa-miR-4750-3p 112-119 -0.392 98.0 -0.392 99.0 noncon
hsa-miR-127-5p 1476-1483 -0.372 98.0 -0.372 99.0 noncon
hsa-miR-532-3p 18-25 -0.372 99.0 -0.372 99.0 noncon
hsa-miR-1976 447-454 -0.37 98.0 -0.37 99.0 noncon
hsa-miR-671-5p 1128-1135 -0.368 97.0 -0.368 98.0 noncon
hsa-miR-548q 1403-1410 -0.354 98.0 -0.354 99.0 noncon
hsa-miR-6868-5p 1191-1198 -0.348 98.0 -0.348 98.0 noncon
hsa-miR-770-5p 1530-1536 -0.34 97.0 -0.34 98.0 noncon
hsa-miR-4712-5p 1530-1536 -0.34 97.0 -0.34 98.0 noncon
hsa-miR-6747-3p 445-452 -0.336 97.0 -0.336 98.0 noncon
hsa-miR-367-5p 63-70 -0.332 97.0 -0.332 97.0 noncon
hsa-miR-9-5p 1065-1071 -0.331 98.0 -0.331 98.0 con
hsa-miR-2110 545-552 -0.323 97.0 -0.323 98.0 noncon
hsa-miR-6792-3p 927-934 -0.314 96.0 -0.314 97.0 noncon
hsa-miR-4784 383-390 -0.302 97.0 -0.302 97.0 noncon
hsa-miR-3150b-3p 383-390 -0.302 97.0 -0.302 98.0 noncon
hsa-miR-584-5p 522-529 -0.297 96.0 -0.297 97.0 noncon
hsa-miR-519d-3p 629-635 -0.291 97.0 -0.291 98.0 con
hsa-miR-4652-3p 160-167 -0.29 99.0 -0.29 99.0 noncon
hsa-miR-425-5p 535-542 -0.289 98.0 -0.289 98.0 noncon
hsa-miR-519c-3p 628-634 -0.284 97.0 -0.284 98.0 noncon
hsa-miR-5001-3p 228-235 -0.282 95.0 -0.282 97.0 noncon
hsa-miR-4279 453-459 -0.281 97.0 -0.281 97.0 noncon
hsa-miR-4771 654-660 -0.275 94.0 -0.275 96.0 noncon
hsa-miR-519b-3p 628-634 -0.273 96.0 -0.273 97.0 noncon
hsa-miR-519a-3p 628-634 -0.273 96.0 -0.273 97.0 noncon
hsa-miR-3150b-3p 1023-1030 -0.269 96.0 -0.269 96.0 noncon
hsa-miR-4784 1023-1030 -0.269 96.0 -0.269 96.0 noncon
hsa-miR-5092 1610-1616 -0.267 97.0 -0.267 98.0 noncon
hsa-miR-526b-3p 629-635 -0.265 98.0 -0.265 99.0 con
hsa-miR-20a-5p 629-635 -0.261 97.0 -0.261 98.0 con
hsa-miR-15a-3p 704-710 -0.259 97.0 -0.259 98.0 noncon
hsa-miR-455-3p.1 103-110 -0.256 96.0 -0.256 97.0 noncon
hsa-miR-4689 384-390 -0.256 95.0 -0.256 96.0 noncon
hsa-miR-6772-3p 911-918 -0.253 93.0 -0.253 95.0 noncon