GlyClass

GlycoEnzDB
Comprehensive glycoEnzyme database and dynamic pathway generation

Description: The GlycoEnzDB is a manually curated glycoEnzyme database, primarily focused on humans. It covers 390 enzymes across 28 pathway maps. Facilities are also available to create custom glycosylation reaction pathways using experimental data in SBML format and for pathway simulation
Contacts: Sriram Neelamegham (neel@buffalo.edu), Yusen Zhou (yusenzho@buffalo.edu) or Ted Groth (tgroth@buffalo.edu).

GlycoEnzDB
Create glycosylation pathway
Custom database generation
Instructions/Reference
 
Pathway Map
DSEL



General Information
Gene Symbol:   
DSEL
Protein name:   
Dermatan-sulfate epimerase-like protein
Enzyme Class:   
Epimerase
CAZy Family:   
E.C. No:   
5.1.3.19
Organs:   
Reaction (SNFG):   
→
Reaction (IUBMB):   
No specified reaction.
About

• DS is derived from CS by the epimerization of GlcA to IdoA. By definition, a single iduronic acid is sufficient to name the chain DS
• Dermatan sulfate epimerase 1 and 2 epimerize glucuronic acid to iduronic acid in DS
• After epimerization of the chain, the C2 hydroxyl group of GlcA/IdoA and the C4 and/or C6 hydroxyl group of GalNAc can be sulfated, giving rise to seven different disaccharide structures
• Disregulation of the epimerase activity results in the propagation of various inflammatory and oncological disorders

Related Pathway
KEGG ORTHOLOGY:   
KEGG Pathway:   
No Record 
Reactome:   
R-HSA-2022923
Related Database
Organism:   
Homo sapiens
Uniprot:   
Q8IZU8 
NCBI GeneID:   
92126
GlyMap GeneID:   
92126
DNA aRefSeq:   
NM_032160.2 
Protein RefSeq:   
NP_115536.1 
BRENDA:   
Kinetic info.
OMIM number:   
611125
GeneCards database:   
DSEL
Enzyme Specificity
Glycan Type:  
     GAG
Compartment:  
     
Substrate:  
     ...GlcA(b1-3)
Product:  
     ...IdoA(a1-3)
Constraints:  
     !@GlcA(b1-3)Gal(b1-3)Gal(b1-4)Xyl(b1-r)
Simulation Parameters
Mechaelis-Menten parameters (suggested)
VM:       (pmol/cell • hr)       KM:       (pM)
Relative conc of enzymes in compartment.(sum will be normalized to 1)
ER Cis- Medial- Trans- TGN










Expression Data in Tissue {ref. 5} Source from NCBI gene database
adrenal appendix bone marrow brain colon duodenum endometrium esophagus fat gall bladder heart kidney liver lung lymph node ovary pancreas placenta prostate salivary gland skin small intestine spleen stomach testis thyroid urinary bladder 6 4 2 0 RPKM
Expression Data in Cells {ref. 13} Source from Cancer Cell Line Encyclopedia database
Kidney Lung Breast Prostate Large intestine Ovary HL60 haematopoietic and lymphoid tissue Skin Central nervous system 786O A498 ACHN CAKI1 UO31 A549 EKVX HOP62 HOP92 NCIH226 NCIH23 NCIH322 NCIH460 NCIH522 SHP77 BT549 HS578T MCF7 MDAMB231 MDAMB468 T47D DU145 PC3 HCT116 HCT15 HT29 KM12 SW620 OVCAR4 OVCAR8 IGROV1 SKOV3 HL60 K562 RPMI8226 LOXIMVI MALME3M RPMI7951 SKMEL28 SKMEL5 UACC257 UACC62 SF268 SF295 SF539 SNB75 30 20 10 0 Transcripts per million (TPM)
Transcription factor {ref. 10}
No high degree of confidence data
miRNA-GlycoEnzyme Interactome {ref. 9}
miRNA Name Pos. Context Score Frac. Wt. Context Frac. Conserved Quality
hsa-miR-4295 22-29 -0.519 99.0 -0.519 99.0 con
hsa-miR-454-3p 22-29 -0.498 99.0 -0.498 99.0 con
hsa-miR-3666 22-29 -0.495 99.0 -0.495 99.0 con
hsa-miR-301b-3p 22-29 -0.495 99.0 -0.495 99.0 con
hsa-miR-301a-3p 22-29 -0.495 99.0 -0.495 99.0 con
hsa-miR-130b-3p 22-29 -0.487 99.0 -0.487 99.0 con
hsa-miR-130a-3p 22-29 -0.479 99.0 -0.479 99.0 con
hsa-miR-4671-3p 23-30 -0.441 97.0 -0.441 98.0 noncon
hsa-miR-6853-5p 391-398 -0.397 94.0 -0.276 89.0 noncon
hsa-miR-19a-3p 21-27 -0.32 95.0 -0.32 96.0 con
hsa-miR-19b-3p 21-27 -0.32 95.0 -0.32 96.0 con
hsa-miR-208b-5p 4369-4376 -0.285 98.0 -0.201 97.0 noncon
hsa-miR-1273d 4152-4159 -0.266 98.0 -0.188 98.0 noncon
hsa-miR-1252-5p 4264-4271 -0.253 95.0 -0.179 92.0 noncon