GlyClass

GlycoEnzDB
Comprehensive glycoEnzyme database and dynamic pathway generation

Description: The GlycoEnzDB is a manually curated glycoEnzyme database, primarily focused on humans. It covers 390 enzymes across 28 pathway maps. Facilities are also available to create custom glycosylation reaction pathways using experimental data in SBML format and for pathway simulation
Contacts: Sriram Neelamegham (neel@buffalo.edu), Yusen Zhou (yusenzho@buffalo.edu) or Ted Groth (tgroth@buffalo.edu).

GlycoEnzDB
Create glycosylation pathway
Custom database generation
Instructions/Reference
 
Pathway Map
GALNS



General Information
Gene Symbol:   
GALNS
Protein name:   
N-acetylgalactosamine-6-sulfatase
Enzyme Class:   
CAZy Family:   
E.C. No:   
3.1.6.4
Organs:   
Reaction (SNFG):   
→
→
Reaction (IUBMB):   
No specified reaction.
About

• Hydrolyzes the 6 carbon sulfates on GalNAc residues on chondroitin sulfate and the 6 carbon sulfates on Gal residues on keratin sulfate.

Related Pathway
KEGG ORTHOLOGY:   
KEGG Pathway:   
No Record 
Reactome:   
R-HSA-2022857
Related Database
Organism:   
Homo sapiens
Uniprot:   
P34059 
NCBI GeneID:   
2588
GlyMap GeneID:   
2588
DNA aRefSeq:   
NM_000512.4 
Protein RefSeq:   
NP_000503.1 
BRENDA:   
Kinetic info.
OMIM number:   
612222
GeneCards database:   
GALNS
Enzyme Specificity
Glycan Type:  
     GAG
Compartment:  
     
Substrate:  
     GalNAc(b1-4 S-6)GlcA(b1-3) or Gal(b1-4 S-6)GlcNAc(b1-3)
Product:  
     GalNAc(b1-4)GlcA(b1-3) or Gal(b1-4)GlcNAc(b1-3)
Constraints:  
     None
Simulation Parameters
Mechaelis-Menten parameters (suggested)
VM:       (pmol/cell • hr)       KM:       (pM)
Relative conc of enzymes in compartment.(sum will be normalized to 1)
ER Cis- Medial- Trans- TGN










Expression Data in Tissue {ref. 5} Source from NCBI gene database
adrenal appendix bone marrow brain colon duodenum endometrium esophagus fat gall bladder heart kidney liver lung lymph node ovary pancreas placenta prostate salivary gland skin small intestine spleen stomach testis thyroid urinary bladder 6 4 2 0 RPKM
Expression Data in Cells {ref. 13} Source from Cancer Cell Line Encyclopedia database
Kidney Lung Breast Prostate Large intestine Ovary HL60 haematopoietic and lymphoid tissue Skin Central nervous system 786O A498 ACHN CAKI1 UO31 A549 EKVX HOP62 HOP92 NCIH226 NCIH23 NCIH322 NCIH460 NCIH522 SHP77 BT549 HS578T MCF7 MDAMB231 MDAMB468 T47D DU145 PC3 HCT116 HCT15 HT29 KM12 SW620 OVCAR4 OVCAR8 IGROV1 SKOV3 HL60 K562 RPMI8226 LOXIMVI MALME3M RPMI7951 SKMEL28 SKMEL5 UACC257 UACC62 SF268 SF295 SF539 SNB75 60 50 40 30 20 10 0 Transcripts per million (TPM)
Transcription factor {ref. 10}
Transcriptional start site (TSS) 0 200 -2000 -1800 -1600 -1400 -1200 -1000 -800 -600 -400 -200
ENST00000268695 ENST00000562593 ENST00000565364 ENST00000568311 SOX2 CTCF MYB USF1 SP1 ZFHX2 TAL1 EGR2 EGR1 EGR1 CREM ATF2 JUND MAX BHLHE40 USF2 CEBPB USF1 FOSL1 MITF FOS ZNF770
miRNA-GlycoEnzyme Interactome {ref. 9}
miRNA Name Pos. Context Score Frac. Wt. Context Frac. Conserved Quality
hsa-miR-122-3p 430-437 -0.64 99.0 -0.64 99.0 noncon
hsa-miR-4726-5p 478-485 -0.526 98.0 -0.526 99.0 noncon
hsa-miR-4649-5p 189-196 -0.524 97.0 -0.524 97.0 noncon
hsa-miR-4640-5p 478-485 -0.516 98.0 -0.516 99.0 noncon
hsa-miR-6778-3p 675-682 -0.509 99.0 -0.506 99.0 noncon
hsa-miR-4649-5p 225-232 -0.501 96.0 -0.501 97.0 noncon
hsa-miR-6729-5p 189-196 -0.492 96.0 -0.492 97.0 noncon
hsa-miR-6729-5p 225-232 -0.485 96.0 -0.485 96.0 noncon
hsa-miR-3138 589-596 -0.44 97.0 -0.437 98.0 noncon
hsa-miR-4665-5p 84-91 -0.437 97.0 -0.437 98.0 noncon
hsa-miR-6132 41-48 -0.415 98.0 -0.415 98.0 noncon
hsa-miR-3170 276-283 -0.414 98.0 -0.414 98.0 noncon
hsa-miR-7113-5p 604-611 -0.413 98.0 -0.41 99.0 noncon
hsa-miR-3197 199-206 -0.408 98.0 -0.408 98.0 noncon
hsa-miR-6836-5p 41-48 -0.402 97.0 -0.402 98.0 noncon
hsa-miR-6855-5p 276-283 -0.393 98.0 -0.393 98.0 noncon
hsa-miR-1281 625-631 -0.39 95.0 -0.388 96.0 noncon
hsa-miR-3197 235-242 -0.389 97.0 -0.389 98.0 noncon
hsa-miR-7111-5p 83-90 -0.386 93.0 -0.386 95.0 noncon
hsa-miR-4723-5p 83-90 -0.378 93.0 -0.378 94.0 noncon
hsa-miR-5698 83-90 -0.362 92.0 -0.362 94.0 noncon
hsa-miR-6870-5p 83-90 -0.362 92.0 -0.362 94.0 noncon
hsa-miR-212-5p 312-318 -0.361 97.0 -0.361 98.0 noncon
hsa-miR-6721-5p 93-100 -0.358 97.0 -0.358 97.0 noncon
hsa-miR-5572 277-284 -0.358 98.0 -0.358 98.0 noncon
hsa-miR-626 109-116 -0.346 95.0 -0.346 96.0 noncon
hsa-miR-6876-3p 109-116 -0.346 95.0 -0.346 97.0 noncon
hsa-miR-6721-5p 148-155 -0.336 96.0 -0.336 97.0 noncon
hsa-miR-1275 84-91 -0.332 97.0 -0.332 98.0 noncon
hsa-miR-581 411-418 -0.327 97.0 -0.327 98.0 noncon
hsa-miR-544b 245-252 -0.319 98.0 -0.319 98.0 noncon
hsa-miR-3665 115-122 -0.314 95.0 -0.314 96.0 noncon
hsa-miR-6778-3p 624-630 -0.3 96.0 -0.298 97.0 noncon
hsa-miR-4330 286-293 -0.296 98.0 -0.296 98.0 noncon
hsa-miR-1205 117-124 -0.295 98.0 -0.295 98.0 noncon
hsa-miR-4283 551-558 -0.293 93.0 -0.291 94.0 noncon
hsa-miR-4799-5p 660-667 -0.29 99.0 -0.288 99.0 noncon
hsa-miR-939-3p 151-158 -0.29 96.0 -0.29 97.0 noncon
hsa-miR-6884-5p 337-344 -0.284 96.0 -0.284 97.0 noncon
hsa-miR-3918 40-46 -0.283 95.0 -0.283 96.0 noncon
hsa-miR-7109-5p 85-91 -0.282 91.0 -0.282 94.0 noncon
hsa-miR-1207-5p 92-98 -0.278 94.0 -0.278 95.0 noncon
hsa-miR-5189-3p 36-42 -0.274 93.0 -0.274 96.0 noncon
hsa-miR-452-5p 506-513 -0.264 96.0 -0.262 97.0 noncon
hsa-miR-6836-3p 676-682 -0.262 95.0 -0.261 96.0 noncon
hsa-miR-892c-3p 506-513 -0.259 96.0 -0.257 97.0 noncon
hsa-miR-4676-3p 506-513 -0.259 96.0 -0.257 97.0 noncon
hsa-miR-653-3p 483-490 -0.257 96.0 -0.255 97.0 noncon
hsa-miR-544b 340-347 -0.257 96.0 -0.257 97.0 noncon
hsa-miR-6715b-5p 362-369 -0.255 96.0 -0.255 97.0 noncon