GlyClass

GlycoEnzDB
Comprehensive glycoEnzyme database and dynamic pathway generation

Description: The GlycoEnzDB is a manually curated glycoEnzyme database, primarily focused on humans. It covers 390 enzymes across 28 pathway maps. Facilities are also available to create custom glycosylation reaction pathways using experimental data in SBML format and for pathway simulation
Contacts: Sriram Neelamegham (neel@buffalo.edu), Yusen Zhou (yusenzho@buffalo.edu) or Ted Groth (tgroth@buffalo.edu).

GlycoEnzDB
Create glycosylation pathway
Custom database generation
Instructions/Reference
 
Pathway Map
GUSB



General Information
Gene Symbol:   
GUSB
Protein name:   
Beta-glucuronidase
Enzyme Class:   
Glycosidase
CAZy Family:   
GH2
E.C. No:   
3.2.1.31
Tissues:   
Muscular, Epithelial, Connective, Nervous
Organs:   
Reaction (SNFG):   
GUSB
H2O +
→
+
Reaction (IUBMB):   
No specified reaction.
About

• GUSB is required to degrade glycosaminoglycans (GAGs), including heparan sulfate (HS), dermatan sulfate (DS), and chondroitin-4,6-sulfate (CS).
• Mucopolysaccharidosis VII (MPS VII; Sly syndrome) is an autosomal recessive disorder caused by a deficiency of beta-glucuronidase (GUS, EC 3.2.1.31; GUSB). Here, the accumulation of undegraded GAGs in lysosomes of affected tissues leads to mental retardation, short stature, hepatosplenomegaly, bone dysplasia, and hydrops fetalis.

Related Pathway
KEGG ORTHOLOGY:   
KEGG Pathway:   
No Record 
Reactome:   
R-HSA-2024096
Related Database
Organism:   
Homo sapiens
Uniprot:   
P08236 
NCBI GeneID:   
2990
GlyMap GeneID:   
2990
DNA aRefSeq:   
NM_000181.3 
Protein RefSeq:   
NP_000172.2 
BRENDA:   
Kinetic info.
OMIM number:   
611499
GeneCards database:   
GUSB
Enzyme Specificity
Glycan Type:  
     GAG
Compartment:  
     
Substrate:  
      GlcA(b1-3)GalNAc(b1-4)
Product:  
     GalNAc(b1-4)
Constraints:  
     None
Simulation Parameters
Mechaelis-Menten parameters (suggested)
VM:       (pmol/cell • hr)       KM:       (pM)
Relative conc of enzymes in compartment.(sum will be normalized to 1)
ER Cis- Medial- Trans- TGN










Expression Data in Tissue {ref. 5} Source from NCBI gene database
adrenal appendix bone marrow brain colon duodenum endometrium esophagus fat gall bladder heart kidney liver lung lymph node ovary pancreas placenta prostate salivary gland skin small intestine spleen stomach testis thyroid urinary bladder 50 40 30 20 10 0 RPKM
Expression Data in Cells {ref. 13} Source from Cancer Cell Line Encyclopedia database
Kidney Lung Breast Prostate Large intestine Ovary HL60 haematopoietic and lymphoid tissue Skin Central nervous system 786O A498 ACHN CAKI1 UO31 A549 EKVX HOP62 HOP92 NCIH226 NCIH23 NCIH322 NCIH460 NCIH522 SHP77 BT549 HS578T MCF7 MDAMB231 MDAMB468 T47D DU145 PC3 HCT116 HCT15 HT29 KM12 SW620 OVCAR4 OVCAR8 IGROV1 SKOV3 HL60 K562 RPMI8226 LOXIMVI MALME3M RPMI7951 SKMEL28 SKMEL5 UACC257 UACC62 SF268 SF295 SF539 SNB75 200 180 160 140 120 100 80 60 40 20 0 Transcripts per million (TPM)
Transcription factor {ref. 10}
Transcriptional start site (TSS) 0 200 -2000 -1800 -1600 -1400 -1200 -1000 -800 -600 -400 -200
ENST00000304895 ENST00000446111 ENST00000461622 CTCF NKX3-1 VDR STAT5A ERG STAT5A ZNF316 NFE2L2 MAFG NFE2 MAFK BACH2
miRNA-GlycoEnzyme Interactome {ref. 9}
miRNA Name Pos. Context Score Frac. Wt. Context Frac. Conserved Quality
hsa-miR-6777-5p 31-38 -0.675 99.0 -0.675 99.0 noncon
hsa-miR-6889-5p 31-38 -0.673 99.0 -0.673 99.0 noncon
hsa-miR-642b-5p 138-145 -0.517 99.0 -0.516 99.0 noncon
hsa-miR-4255 141-148 -0.491 99.0 -0.49 99.0 noncon
hsa-miR-6503-5p 90-97 -0.475 98.0 -0.475 99.0 noncon
hsa-miR-222-5p 204-211 -0.472 99.0 -0.002 33.0 noncon
hsa-miR-3591-5p 143-150 -0.458 99.0 -0.457 99.0 noncon
hsa-miR-4527 90-97 -0.457 98.0 -0.457 99.0 noncon
hsa-miR-3942-3p 216-223 -0.439 99.0 0.0 36.0 noncon
hsa-miR-6071 58-65 -0.427 99.0 -0.427 99.0 noncon
hsa-miR-587 174-181 -0.409 99.0 -0.407 99.0 noncon
hsa-miR-3190-3p 49-56 -0.38 98.0 -0.38 98.0 noncon
hsa-miR-142-3p.2 142-149 -0.37 98.0 -0.369 98.0 noncon
hsa-miR-30a-3p 206-213 -0.359 99.0 -0.001 44.0 noncon
hsa-miR-30d-3p 206-213 -0.359 99.0 -0.001 44.0 noncon
hsa-miR-30e-3p 206-213 -0.359 99.0 -0.001 44.0 noncon
hsa-miR-665 73-80 -0.357 98.0 -0.357 98.0 noncon
hsa-miR-6726-3p 42-48 -0.354 93.0 -0.354 95.0 noncon
hsa-miR-449b-3p 200-206 -0.319 98.0 -0.318 98.0 noncon
hsa-miR-3663-5p 92-99 -0.294 98.0 -0.294 98.0 noncon
hsa-miR-542-5p 32-38 -0.277 91.0 -0.277 93.0 noncon
hsa-miR-1245b-3p 215-221 -0.266 97.0 0.0 21.0 noncon
hsa-miR-936 204-210 -0.263 93.0 -0.262 95.0 noncon
hsa-miR-5189-3p 188-194 -0.254 92.0 -0.253 95.0 noncon
hsa-miR-199b-3p 204-210 -0.253 93.0 -0.252 95.0 noncon
hsa-miR-199a-3p 204-210 -0.253 93.0 -0.252 95.0 noncon