GlyClass

GlycoEnzDB
Comprehensive glycoEnzyme database and dynamic pathway generation

Description: The GlycoEnzDB is a manually curated glycoEnzyme database, primarily focused on humans. It covers 390 enzymes across 28 pathway maps. Facilities are also available to create custom glycosylation reaction pathways using experimental data in SBML format and for pathway simulation
Contacts: Sriram Neelamegham (neel@buffalo.edu), Yusen Zhou (yusenzho@buffalo.edu) or Ted Groth (tgroth@buffalo.edu).

GlycoEnzDB
Create glycosylation pathway
Custom database generation
Instructions/Reference
 
Pathway Map
HS3ST1



General Information
Gene Symbol:   
HS3ST1
Protein name:   
Heparan sulfate glucosamine 3-O-sulfotransferase 1
Enzyme Class:   
Sulfate synthase
CAZy Family:   
E.C. No:   
2.8.2.23
Organs:   
Reaction (SNFG):   
PAPS +
→
+ PAP
PAPS +
→
+ PAP
PAPS +
→
+ PAP
PAPS +
→
+ PAP
Reaction (IUBMB):   
No specified reaction.
About

• Sulfation of heparan sulfate occurs at C2 of HexA. It also occurs at C3, C6, and NS residues of GlcNAc or GlcNS. HSPGs are major regulators of cell behavior with roles in inflammation, blood coagulation, tumor invasion, and malignancy.
• Seven isoenzymes display HS 3-O-sulfotransferase activity, with all enzymes transferring sulfate to C3 of GlcNAc in heparin and heparan sulfate.
• HS3ST1 sulfates an N-sulfo glucosamine (GlcNS) that has a nonsulfated glucuronic acid (GlcA) or an iduronic acid (IdoA) at the nonreducing end. This modification is necessary for anti-thrombin activity of heparin. HS
• HS3ST-2, 3 (3A and 3B), 4, and 6, all sulfate a GlcNS6S residue that has a 2-O-sulfated iduronic acid (IdoA2S) on the nonreducing end. This does not provide anti-thrombin activity, but serves viral entry for herpes simplex virus type-1.
• The tissue distribution of the different HS3STs is quite different.

Related Pathway
KEGG ORTHOLOGY:   
K01024
KEGG Pathway:   
ko00534 
Reactome:   
R-HSA-2022928
Related Database
Organism:   
Homo sapiens
Uniprot:   
O14792 
NCBI GeneID:   
9957
GlyMap GeneID:   
9957
DNA aRefSeq:   
NM_005114.2 
Protein RefSeq:   
NP_005105.1 
BRENDA:   
Kinetic info.
OMIM number:   
603244
GeneCards database:   
HS3ST1
Enzyme Specificity
Glycan Type:  
     GAG
Compartment:  
     Cis, Medial, Trans, TGN
Substrate:  
     IdoA(a1-4)GlcN(a1-4 [NS]) or IdoA(a1-4)GlcN(a1-4 [6,NS]) or GlcA(a1-4)GlcN(a1-4 [NS]) or GlcA(a1-4)GlcN(a1-4 [6,NS])
Product:  
     IdoA(a1-4)GlcN(a1-4 [3,NS]) or IdoA(a1-4)GlcN(a1-4 [3,6,NS]) or GlcA(a1-4)GlcN(a1-4 [3,NS]) or GlcA(a1-4)GlcN(a1-4 [3,6,NS])
Simulation Parameters
Mechaelis-Menten parameters (suggested)
VM:       (pmol/cell • hr)       KM:       (pM)
Relative conc of enzymes in compartment.(sum will be normalized to 1)
ER Cis- Medial- Trans- TGN










Expression Data in Tissue {ref. 5} Source from NCBI gene database
adrenal appendix bone marrow brain colon duodenum endometrium esophagus fat gall bladder heart kidney liver lung lymph node ovary pancreas placenta prostate salivary gland skin small intestine spleen stomach testis thyroid urinary bladder 4 2 0 RPKM
Expression Data in Cells {ref. 13} Source from Cancer Cell Line Encyclopedia database
Kidney Lung Breast Prostate Large intestine Ovary HL60 haematopoietic and lymphoid tissue Skin Central nervous system 786O A498 ACHN CAKI1 UO31 A549 EKVX HOP62 HOP92 NCIH226 NCIH23 NCIH322 NCIH460 NCIH522 SHP77 BT549 HS578T MCF7 MDAMB231 MDAMB468 T47D DU145 PC3 HCT116 HCT15 HT29 KM12 SW620 OVCAR4 OVCAR8 IGROV1 SKOV3 HL60 K562 RPMI8226 LOXIMVI MALME3M RPMI7951 SKMEL28 SKMEL5 UACC257 UACC62 SF268 SF295 SF539 SNB75 20 10 0 Transcripts per million (TPM)
Transcription factor {ref. 10}
No high degree of confidence data
miRNA-GlycoEnzyme Interactome {ref. 9}
miRNA Name Pos. Context Score Frac. Wt. Context Frac. Conserved Quality
hsa-miR-4290 182-189 -0.57 99.0 -0.57 99.0 noncon
hsa-miR-5091 4340-4347 -0.534 97.0 -0.276 90.0 noncon
hsa-miR-6826-3p 55-62 -0.525 99.0 -0.525 99.0 noncon
hsa-miR-6724-5p 5845-5852 -0.521 99.0 -0.27 95.0 noncon
hsa-miR-6773-5p 5845-5852 -0.516 99.0 -0.268 94.0 noncon
hsa-miR-3144-3p 86-93 -0.481 99.0 -0.481 99.0 noncon
hsa-miR-205-5p 238-245 -0.456 99.0 -0.456 99.0 con
hsa-miR-4303 16-23 -0.423 99.0 -0.423 99.0 noncon
hsa-miR-4276 257-264 -0.423 99.0 -0.423 99.0 noncon
hsa-miR-1246 105-112 -0.397 99.0 -0.397 99.0 noncon
hsa-miR-3147 5847-5854 -0.375 98.0 -0.199 94.0 noncon
hsa-miR-3157-5p 5736-5743 -0.375 98.0 -0.199 94.0 noncon
hsa-miR-4493 711-718 -0.366 97.0 -0.265 95.0 noncon
hsa-miR-18a-3p 1036-1043 -0.358 98.0 -0.191 94.0 noncon
hsa-miR-6805-5p 3262-3268 -0.345 93.0 -0.184 82.0 noncon
hsa-miR-6716-5p 555-562 -0.343 97.0 -0.249 94.0 noncon
hsa-miR-365b-3p 185-191 -0.335 96.0 -0.335 98.0 con
hsa-miR-365a-3p 185-191 -0.335 96.0 -0.335 98.0 con
hsa-miR-5705 5454-5460 -0.334 90.0 -0.178 80.0 noncon
hsa-miR-6852-3p 4656-4663 -0.329 95.0 -0.176 87.0 noncon
hsa-miR-3909 4656-4663 -0.329 95.0 -0.176 87.0 noncon
hsa-miR-4682 4277-4284 -0.327 98.0 -0.175 94.0 noncon
hsa-miR-296-3p 4231-4238 -0.327 98.0 -0.175 93.0 noncon
hsa-miR-601 5561-5568 -0.318 93.0 -0.17 82.0 noncon
hsa-miR-429 126-133 -0.313 98.0 -0.313 99.0 con
hsa-miR-200b-3p 126-133 -0.313 98.0 -0.313 99.0 con
hsa-miR-200c-3p 126-133 -0.313 98.0 -0.313 99.0 con
hsa-miR-6852-5p 1383-1390 -0.31 97.0 -0.166 91.0 noncon
hsa-miR-6728-3p 5679-5686 -0.31 97.0 -0.166 93.0 noncon
hsa-miR-760 1573-1580 -0.304 94.0 -0.163 84.0 noncon
hsa-miR-3923 153-159 -0.302 96.0 -0.302 97.0 noncon
hsa-miR-1290 103-110 -0.3 97.0 -0.3 98.0 noncon
hsa-miR-6755-5p 5499-5506 -0.296 92.0 -0.159 83.0 noncon
hsa-miR-571 324-331 -0.294 94.0 -0.214 91.0 noncon
hsa-miR-1237-5p 320-326 -0.293 92.0 -0.293 94.0 noncon
hsa-miR-6722-3p 5258-5264 -0.292 94.0 -0.157 83.0 noncon
hsa-miR-3976 231-238 -0.291 96.0 -0.291 97.0 noncon
hsa-miR-19a-3p 660-667 -0.288 93.0 -0.21 89.0 noncon
hsa-miR-4697-5p 320-326 -0.285 91.0 -0.285 93.0 noncon
hsa-miR-4471 1005-1012 -0.283 93.0 -0.153 86.0 noncon
hsa-miR-19b-3p 660-667 -0.28 93.0 -0.204 89.0 noncon
hsa-miR-6775-3p 2527-2534 -0.277 91.0 -0.149 79.0 noncon
hsa-miR-3140-5p 591-598 -0.277 97.0 -0.202 96.0 noncon
hsa-miR-5697 169-175 -0.275 98.0 -0.275 98.0 noncon
hsa-miR-106a-3p 354-361 -0.274 97.0 -0.2 96.0 noncon
hsa-miR-378c 3698-3705 -0.274 95.0 -0.148 87.0 noncon
hsa-miR-3529-5p 2328-2335 -0.274 94.0 -0.148 88.0 noncon
hsa-miR-5193 1418-1425 -0.269 93.0 -0.145 86.0 noncon
hsa-miR-5585-5p 115-121 -0.267 95.0 -0.267 97.0 noncon
hsa-miR-3168 5924-5931 -0.266 99.0 -0.144 96.0 noncon
hsa-miR-378f 3698-3705 -0.266 95.0 -0.144 87.0 noncon
hsa-miR-378a-3p 3698-3705 -0.266 94.0 -0.144 87.0 noncon
hsa-miR-422a 3698-3705 -0.266 94.0 -0.144 87.0 noncon
hsa-miR-378i 3698-3705 -0.266 94.0 -0.144 87.0 noncon
hsa-miR-378h 3698-3705 -0.266 94.0 -0.144 87.0 noncon
hsa-miR-378e 3698-3705 -0.266 94.0 -0.144 87.0 noncon
hsa-miR-378d 3698-3705 -0.266 94.0 -0.144 87.0 noncon
hsa-miR-4662b 5872-5879 -0.262 97.0 -0.142 92.0 noncon
hsa-miR-6861-3p 2350-2356 -0.261 90.0 -0.141 84.0 noncon
hsa-miR-6795-3p 55-61 -0.261 96.0 -0.261 97.0 noncon
hsa-miR-221-3p 5879-5886 -0.26 93.0 -0.141 85.0 noncon
hsa-miR-30e-3p 259-266 -0.26 98.0 -0.26 98.0 noncon
hsa-miR-1909-3p 5258-5264 -0.256 91.0 -0.139 80.0 noncon
hsa-miR-30a-3p 259-266 -0.252 97.0 -0.252 98.0 noncon
hsa-miR-30d-3p 259-266 -0.252 97.0 -0.252 98.0 noncon
hsa-miR-6792-3p 4387-4394 -0.25 93.0 -0.135 84.0 noncon
hsa-miR-378b 3698-3705 -0.25 94.0 -0.135 86.0 noncon