GlyClass

GlycoEnzDB
Comprehensive glycoEnzyme database and dynamic pathway generation

Description: The GlycoEnzDB is a manually curated glycoEnzyme database, primarily focused on humans. It covers 390 enzymes across 28 pathway maps. Facilities are also available to create custom glycosylation reaction pathways using experimental data in SBML format and for pathway simulation
Contacts: Sriram Neelamegham (neel@buffalo.edu), Yusen Zhou (yusenzho@buffalo.edu) or Ted Groth (tgroth@buffalo.edu).

GlycoEnzDB
Create glycosylation pathway
Custom database generation
Instructions/Reference
 
Pathway Map
HS3ST2



General Information
Gene Symbol:   
HS3ST2
Protein name:   
Heparan sulfate glucosamine 3-O-sulfotransferase 2
Enzyme Class:   
Sulfate synthase
CAZy Family:   
E.C. No:   
2.8.2.29
Organs:   
Reaction (SNFG):   
PAPS +
→
+ PAP
PAPS +
→
+ PAP
Reaction (IUBMB):   
No specified reaction.
About

• Sulfation of heparan sulfate occurs at C2 of HexA. It also occurs at C3, C6, and NS residues of GlcNAc or GlcNS. HSPGs are major regulators of cell behavior with roles in inflammation, blood coagulation, tumor invasion, and malignancy.
• Seven isoenzymes display HS 3-O-sulfotransferase activity, with all enzymes transferring sulfate to C3 of GlcNAc in heparin and heparan sulfate.
• HS3ST1 sulfates an N-sulfo glucosamine (GlcNS) that has a nonsulfated glucuronic acid (GlcA) or an iduronic acid (IdoA) at the nonreducing end. This modification is necessary for anti-thrombin activity of heparin. HS
• HS3ST-2, 3 (3A and 3B), 4, and 6, all sulfate a GlcNS6S residue that has a 2-O-sulfated iduronic acid (IdoA2S) on the nonreducing end. This does not provide anti-thrombin activity, but serves viral entry for herpes simplex virus type-1.
• The tissue distribution of the different HS3STs is quite different.

Related Pathway
KEGG ORTHOLOGY:   
K07808
KEGG Pathway:   
ko00534 
Reactome:   
R-HSA-2022928
Related Database
Organism:   
Homo sapiens
Uniprot:   
Q9Y278 
NCBI GeneID:   
9956
GlyMap GeneID:   
9956
DNA aRefSeq:   
NM_006043.1 
Protein RefSeq:   
NP_006034.1 
BRENDA:   
Kinetic info.
OMIM number:   
604056
GeneCards database:   
HS3ST2
Enzyme Specificity
Glycan Type:  
     GAG
Compartment:  
     Cis, Medial, Trans, TGN
Substrate:  
     IdoA(a1-4 [2S])GlcN(a1-4 [2S]) or IdoA(a1-4 [2S])GlcN(a1-4 [2,6S])
Product:  
     IdoA(a1-4 [2S])GlcN(a1-4 [2,3S]) or IdoA(a1-4 [2S])GlcN(a1-4 [2,3,6S])
Constraints:  
     nGlcNAc(a1-4 NS-2)>=1
Simulation Parameters
Mechaelis-Menten parameters (suggested)
VM:       (pmol/cell • hr)       KM:       (pM)
Relative conc of enzymes in compartment.(sum will be normalized to 1)
ER Cis- Medial- Trans- TGN










Expression Data in Tissue {ref. 5} Source from NCBI gene database
adrenal appendix bone marrow brain colon duodenum endometrium esophagus fat gall bladder heart kidney liver lung lymph node ovary pancreas placenta prostate salivary gland skin small intestine spleen stomach testis thyroid urinary bladder 20 10 0 RPKM
Expression Data in Cells {ref. 13} Source from Cancer Cell Line Encyclopedia database
Kidney Lung Breast Prostate Large intestine Ovary HL60 haematopoietic and lymphoid tissue Skin Central nervous system 786O A498 ACHN CAKI1 UO31 A549 EKVX HOP62 HOP92 NCIH226 NCIH23 NCIH322 NCIH460 NCIH522 SHP77 BT549 HS578T MCF7 MDAMB231 MDAMB468 T47D DU145 PC3 HCT116 HCT15 HT29 KM12 SW620 OVCAR4 OVCAR8 IGROV1 SKOV3 HL60 K562 RPMI8226 LOXIMVI MALME3M RPMI7951 SKMEL28 SKMEL5 UACC257 UACC62 SF268 SF295 SF539 SNB75 2 0 Transcripts per million (TPM)
Transcription factor {ref. 10}
No high degree of confidence data
miRNA-GlycoEnzyme Interactome {ref. 9}
miRNA Name Pos. Context Score Frac. Wt. Context Frac. Conserved Quality
hsa-miR-100-5p 683-690 -0.76 99.0 -0.76 99.0 con
hsa-miR-99b-5p 683-690 -0.747 99.0 -0.747 99.0 con
hsa-miR-99a-5p 683-690 -0.744 99.0 -0.744 99.0 con
hsa-miR-4743-5p 530-537 -0.562 99.0 -0.562 99.0 noncon
hsa-miR-6515-5p 78-85 -0.484 99.0 -0.484 99.0 noncon
hsa-miR-4763-3p 173-180 -0.465 99.0 -0.465 99.0 noncon
hsa-miR-1207-5p 173-180 -0.463 99.0 -0.463 99.0 noncon
hsa-miR-1249-5p 77-84 -0.438 99.0 -0.438 99.0 noncon
hsa-miR-3928-5p 426-433 -0.435 99.0 -0.435 99.0 noncon
hsa-miR-6797-5p 77-84 -0.43 98.0 -0.43 99.0 noncon
hsa-miR-6806-3p 426-433 -0.419 99.0 -0.419 99.0 noncon
hsa-miR-498 605-612 -0.407 99.0 -0.407 99.0 noncon
hsa-miR-6794-3p 293-300 -0.401 98.0 -0.401 98.0 noncon
hsa-miR-4717-3p 741-748 -0.401 99.0 -0.401 99.0 noncon
hsa-miR-8080 768-775 -0.399 99.0 -0.399 99.0 noncon
hsa-miR-4468 33-40 -0.375 98.0 -0.375 98.0 noncon
hsa-miR-4786-5p 592-599 -0.374 98.0 -0.374 98.0 noncon
hsa-miR-1197 767-773 -0.369 99.0 -0.369 99.0 con
hsa-miR-1273e 200-207 -0.363 98.0 -0.363 98.0 noncon
hsa-miR-363-5p 376-383 -0.356 97.0 -0.356 98.0 noncon
hsa-miR-6805-5p 101-107 -0.354 94.0 -0.354 95.0 noncon
hsa-miR-6128 237-244 -0.352 99.0 -0.352 99.0 noncon
hsa-miR-3654 228-235 -0.349 99.0 -0.349 99.0 noncon
hsa-miR-1299 238-245 -0.342 98.0 -0.342 98.0 noncon
hsa-miR-140-3p.1 653-660 -0.342 98.0 -0.342 98.0 con
hsa-miR-4259 110-116 -0.336 96.0 -0.336 97.0 noncon
hsa-miR-6745 376-383 -0.319 96.0 -0.319 97.0 noncon
hsa-miR-1233-3p 25-31 -0.318 97.0 -0.318 98.0 noncon
hsa-miR-6764-3p 163-170 -0.318 98.0 -0.318 98.0 noncon
hsa-miR-218-5p 664-670 -0.312 96.0 -0.312 97.0 con
hsa-miR-505-5p 93-100 -0.306 97.0 -0.306 98.0 noncon
hsa-miR-6824-3p 163-170 -0.302 98.0 -0.302 98.0 noncon
hsa-miR-6133 76-82 -0.3 95.0 -0.3 96.0 noncon
hsa-miR-6127 76-82 -0.3 95.0 -0.3 96.0 noncon
hsa-miR-99a-3p 702-708 -0.3 98.0 -0.3 99.0 noncon
hsa-miR-653-3p 573-579 -0.291 97.0 -0.291 98.0 noncon
hsa-miR-3161 491-498 -0.29 98.0 -0.29 98.0 noncon
hsa-miR-8071 375-381 -0.285 95.0 -0.285 96.0 noncon
hsa-miR-3663-5p 161-168 -0.285 98.0 -0.285 98.0 noncon
hsa-miR-3192-5p 59-66 -0.284 98.0 -0.284 98.0 noncon
hsa-miR-6130 76-82 -0.278 94.0 -0.278 95.0 noncon
hsa-miR-99b-3p 702-708 -0.278 98.0 -0.278 99.0 noncon
hsa-miR-4510 76-82 -0.278 93.0 -0.278 95.0 noncon
hsa-miR-6129 76-82 -0.278 94.0 -0.278 95.0 noncon
hsa-miR-4270 650-656 -0.277 96.0 -0.277 97.0 noncon
hsa-miR-6510-5p 645-651 -0.276 96.0 -0.276 97.0 noncon
hsa-miR-4494 192-198 -0.274 96.0 -0.274 97.0 noncon
hsa-miR-124-5p 348-355 -0.273 93.0 -0.273 95.0 noncon
hsa-miR-4635 437-444 -0.273 98.0 -0.273 99.0 noncon
hsa-miR-760 583-589 -0.272 92.0 -0.272 94.0 noncon
hsa-miR-27a-5p 142-148 -0.271 93.0 -0.271 95.0 noncon
hsa-miR-4419a 76-82 -0.267 93.0 -0.267 94.0 noncon
hsa-miR-6854-5p 424-430 -0.261 96.0 -0.261 97.0 noncon
hsa-miR-4683 73-79 -0.261 95.0 -0.261 96.0 noncon
hsa-miR-4441 643-649 -0.261 96.0 -0.261 97.0 noncon
hsa-miR-4270 643-649 -0.26 95.0 -0.26 96.0 noncon
hsa-miR-4721 477-483 -0.257 91.0 -0.257 93.0 noncon
hsa-miR-1233-3p 15-21 -0.253 95.0 -0.253 96.0 noncon
hsa-miR-6742-3p 379-386 -0.251 97.0 -0.251 97.0 noncon
hsa-miR-127-5p 427-433 -0.251 95.0 -0.251 97.0 noncon