GlyClass

GlycoEnzDB
Comprehensive glycoEnzyme database and dynamic pathway generation

Description: The GlycoEnzDB is a manually curated glycoEnzyme database, primarily focused on humans. It covers 390 enzymes across 28 pathway maps. Facilities are also available to create custom glycosylation reaction pathways using experimental data in SBML format and for pathway simulation
Contacts: Sriram Neelamegham (neel@buffalo.edu), Yusen Zhou (yusenzho@buffalo.edu) or Ted Groth (tgroth@buffalo.edu).

GlycoEnzDB
Create glycosylation pathway
Custom database generation
Instructions/Reference
 
Pathway Map
HS3ST3A1



General Information
Gene Symbol:   
HS3ST3A1
Protein name:   
Heparan sulfate glucosamine 3-O-sulfotransferase 3A1
Enzyme Class:   
Sulfate synthase
CAZy Family:   
E.C. No:   
2.8.2.30
Organs:   
Reaction (SNFG):   
PAPS +
→
+ PAP
PAPS +
→
+ PAP
Reaction (IUBMB):   
No specified reaction.
About

• Sulfation of heparan sulfate occurs at C2 of HexA. It also occurs at C3, C6, and NS residues of GlcNAc or GlcNS. HSPGs are major regulators of cell behavior with roles in inflammation, blood coagulation, tumor invasion, and malignancy.
• Seven isoenzymes display HS 3-O-sulfotransferase activity, with all enzymes transferring sulfate to C3 of GlcNAc in heparin and heparan sulfate.
• HS3ST1 sulfates an N-sulfo glucosamine (GlcNS) that has a nonsulfated glucuronic acid (GlcA) or an iduronic acid (IdoA) at the nonreducing end. This modification is necessary for anti-thrombin activity of heparin. HS
• HS3ST-2, 3 (3A and 3B), 4, and 6, all sulfate a GlcNS6S residue that has a 2-O-sulfated iduronic acid (IdoA2S) on the nonreducing end. This does not provide anti-thrombin activity, but serves viral entry for herpes simplex virus type-1.
• The tissue distribution of the different HS3STs is quite different.

Related Pathway
KEGG ORTHOLOGY:   
K07809
KEGG Pathway:   
ko00534 
Reactome:   
R-HSA-2022928
Related Database
Organism:   
Homo sapiens
Uniprot:   
Q9Y663 
NCBI GeneID:   
9955
GlyMap GeneID:   
9955
DNA aRefSeq:   
NM_006042.2 
Protein RefSeq:   
NP_006033.1 
BRENDA:   
Kinetic info.
OMIM number:   
604057
GeneCards database:   
HS3ST3A1
Enzyme Specificity
Glycan Type:  
     GAG
Compartment:  
     Cis, Medial, Trans, TGN
Substrate:  
     IdoA(a1-4 [2S])GlcN(a1-4 [2S]) or IdoA(a1-4 [2S])GlcN(a1-4 [2,6S])
Product:  
     IdoA(a1-4 [2S])GlcN(a1-4 [2,3S]) or IdoA(a1-4 [2S])GlcN(a1-4 [2,3,6S])
Simulation Parameters
Mechaelis-Menten parameters (suggested)
VM:       (pmol/cell • hr)       KM:       (pM)
Relative conc of enzymes in compartment.(sum will be normalized to 1)
ER Cis- Medial- Trans- TGN










Expression Data in Tissue {ref. 5} Source from NCBI gene database
adrenal appendix bone marrow brain colon duodenum endometrium esophagus fat gall bladder heart kidney liver lung lymph node ovary pancreas placenta prostate salivary gland skin small intestine spleen stomach testis thyroid urinary bladder 4 2 0 RPKM
Expression Data in Cells {ref. 13} Source from Cancer Cell Line Encyclopedia database
Kidney Lung Breast Prostate Large intestine Ovary HL60 haematopoietic and lymphoid tissue Skin Central nervous system 786O A498 ACHN CAKI1 UO31 A549 EKVX HOP62 HOP92 NCIH226 NCIH23 NCIH322 NCIH460 NCIH522 SHP77 BT549 HS578T MCF7 MDAMB231 MDAMB468 T47D DU145 PC3 HCT116 HCT15 HT29 KM12 SW620 OVCAR4 OVCAR8 IGROV1 SKOV3 HL60 K562 RPMI8226 LOXIMVI MALME3M RPMI7951 SKMEL28 SKMEL5 UACC257 UACC62 SF268 SF295 SF539 SNB75 50 40 30 20 10 0 Transcripts per million (TPM)
Transcription factor {ref. 10}
Transcriptional start site (TSS) 0 200 -2000 -1800 -1600 -1400 -1200 -1000 -800 -600 -400 -200
ENST00000284110 ATF2 FLI1 EGR3 RUNX1 ESR1 GLIS1 YY1 NR2F2 SP1 FLI1 ZBTB26 FOXP1 KLF9 EGR3 ZBTB42 ZNF76 ZNF341 ZNF341 ZNF35 ZNF600 ZNF600 ZNF317 ZNF549 IKZF1 KLF9 MAX SP2 SP2 SP2 ZNF263 GATA3 ZBTB17 ZBTB6 ZNF554 ZNF467 PRDM10 RUNX1 ZFP64 ZBTB20 GATA1 MYCN ZNF335 GLIS2 ZEB2 PBX2 PKNOX1 PBX3 GABPA MYC ZNF778 KLF8 SMAD1 SP2 FOXH1 SP4 ZBTB7A ZBTB7A ZNF223 YY1 KLF1 SP1 KLF15 KLF15 ZNF449 ZNF449 SP3 SP3 SP2 NEUROD1 PATZ1 ZNF320 NANOG SMAD3 SMAD4 EGR1 PAX5 ETV1 KLF12 VEZF1 ZBTB26 MTA1 MAZ ATF7 ZNF629 ATF2 JUND KMT2A CREM CREM E2F1 E2F1 ATF3 ZNF574 ZNF574 ATF1 TBP ZFP64 BHLHE40 BHLHE40 ZSCAN22 MAX USF2 CTCFL GLIS1 FOXM1 MNT MNT MBD1 MBD1 MXI1 NONO
miRNA-GlycoEnzyme Interactome {ref. 9}
miRNA Name Pos. Context Score Frac. Wt. Context Frac. Conserved Quality
hsa-miR-3196 141-148 -0.676 98.0 -0.676 98.0 noncon
hsa-miR-564 388-395 -0.664 99.0 -0.401 96.0 noncon
hsa-miR-6816-5p 141-148 -0.663 98.0 -0.663 98.0 noncon
hsa-miR-3180-3p 141-148 -0.655 98.0 -0.655 98.0 noncon
hsa-miR-3180 141-148 -0.655 98.0 -0.655 98.0 noncon
hsa-miR-517a-3p 1503-1510 -0.653 98.0 -0.303 87.0 noncon
hsa-miR-517c-3p 1503-1510 -0.653 98.0 -0.303 87.0 noncon
hsa-miR-517b-3p 1503-1510 -0.653 98.0 -0.303 87.0 noncon
hsa-miR-3918 341-348 -0.596 99.0 -0.363 98.0 noncon
hsa-miR-4536-3p 1502-1509 -0.557 98.0 -0.263 92.0 noncon
hsa-miR-4783-3p 2227-2234 -0.555 97.0 -0.099 57.0 noncon
hsa-miR-433-5p 3055-3062 -0.467 98.0 -0.085 62.0 noncon
hsa-miR-549a 274-281 -0.464 98.0 -0.464 99.0 noncon
hsa-miR-656-5p 3719-3726 -0.429 97.0 -0.079 75.0 noncon
hsa-miR-555 1013-1020 -0.411 97.0 -0.223 91.0 noncon
hsa-miR-5010-5p 1381-1388 -0.385 97.0 -0.188 86.0 noncon
hsa-miR-3170 1875-1882 -0.38 98.0 -0.185 91.0 noncon
hsa-miR-4512 340-346 -0.379 98.0 -0.238 95.0 noncon
hsa-miR-3661 1885-1892 -0.372 97.0 -0.182 88.0 noncon
hsa-miR-6822-5p 648-655 -0.371 99.0 -0.203 96.0 noncon
hsa-miR-4765 1394-1401 -0.366 98.0 -0.179 94.0 noncon
hsa-miR-6750-5p 648-655 -0.363 99.0 -0.199 96.0 noncon
hsa-miR-4681 385-391 -0.362 94.0 -0.228 85.0 noncon
hsa-miR-631 1885-1892 -0.358 96.0 -0.175 86.0 noncon
hsa-miR-193b-5p 1874-1881 -0.352 95.0 -0.172 86.0 noncon
hsa-miR-6769b-3p 1488-1495 -0.35 97.0 -0.171 92.0 noncon
hsa-miR-3127-5p 342-348 -0.346 96.0 -0.218 92.0 noncon
hsa-miR-3612 3881-3888 -0.344 98.0 -0.065 69.0 noncon
hsa-miR-26b-3p 2143-2150 -0.344 97.0 -0.169 91.0 noncon
hsa-miR-628-5p 1537-1544 -0.343 98.0 -0.168 93.0 noncon
hsa-miR-4326 1491-1498 -0.343 97.0 -0.168 90.0 noncon
hsa-miR-6855-5p 1875-1882 -0.343 97.0 -0.168 89.0 noncon
hsa-miR-4723-3p 1488-1495 -0.342 97.0 -0.168 91.0 noncon
hsa-miR-5047 1974-1981 -0.341 97.0 -0.167 91.0 noncon
hsa-miR-1301-3p 1974-1981 -0.341 97.0 -0.167 91.0 noncon
hsa-miR-4293 4615-4622 -0.34 99.0 -0.064 84.0 noncon
hsa-miR-640 177-184 -0.34 97.0 -0.34 98.0 noncon
hsa-miR-154-5p 1285-1292 -0.334 98.0 -0.184 94.0 noncon
hsa-miR-1267 181-188 -0.333 98.0 -0.333 98.0 noncon
hsa-miR-3675-5p 143-149 -0.33 95.0 -0.33 97.0 noncon
hsa-miR-4637 417-424 -0.325 97.0 -0.205 94.0 noncon
hsa-miR-942-5p 60-67 -0.322 98.0 -0.322 98.0 noncon
hsa-miR-4684-5p 58-65 -0.318 98.0 -0.318 98.0 noncon
hsa-miR-6839-3p 1562-1569 -0.313 96.0 -0.154 89.0 noncon
hsa-miR-7155-5p 4069-4076 -0.312 97.0 -0.059 74.0 noncon
hsa-miR-891a-3p 390-396 -0.31 96.0 -0.196 94.0 noncon
hsa-miR-891a-3p 1939-1946 -0.309 96.0 -0.153 90.0 noncon
hsa-miR-205-5p 1782-1789 -0.308 97.0 -0.152 93.0 noncon
hsa-miR-4724-5p 1295-1302 -0.305 98.0 -0.168 96.0 noncon
hsa-miR-650 3881-3888 -0.304 97.0 -0.058 68.0 noncon
hsa-miR-6760-5p 376-382 -0.301 97.0 -0.191 93.0 noncon
hsa-miR-6807-3p 1701-1708 -0.3 98.0 -0.148 94.0 noncon
hsa-miR-548g-3p 69-76 -0.3 99.0 -0.3 99.0 noncon
hsa-miR-610 2018-2025 -0.295 96.0 -0.146 84.0 noncon
hsa-miR-1306-5p 580-587 -0.295 96.0 -0.163 90.0 noncon
hsa-miR-217 1701-1708 -0.295 98.0 -0.146 94.0 noncon
hsa-miR-155-5p 1462-1469 -0.295 95.0 -0.146 87.0 con
hsa-miR-181d-5p 115-121 -0.293 97.0 -0.293 97.0 con
hsa-miR-136-5p 2659-2666 -0.291 97.0 -0.056 77.0 noncon
hsa-miR-5194 779-786 -0.29 96.0 -0.16 88.0 noncon
hsa-miR-6861-5p 249-255 -0.287 97.0 -0.287 97.0 noncon
hsa-miR-5092 175-181 -0.287 98.0 -0.287 98.0 noncon
hsa-miR-543 116-123 -0.287 99.0 -0.287 99.0 con
hsa-miR-3121-5p 2158-2165 -0.286 96.0 -0.109 89.0 noncon
hsa-miR-520g-5p 4699-4705 -0.284 95.0 -0.054 71.0 noncon
hsa-miR-181a-5p 115-121 -0.282 96.0 -0.282 97.0 con
hsa-miR-181b-5p 115-121 -0.282 96.0 -0.282 97.0 con
hsa-miR-181c-5p 115-121 -0.282 96.0 -0.282 97.0 con
hsa-miR-6878-5p 375-381 -0.281 95.0 -0.178 89.0 noncon
hsa-miR-615-5p 4188-4194 -0.28 92.0 -0.054 61.0 noncon
hsa-miR-6738-5p 779-786 -0.279 95.0 -0.155 87.0 noncon
hsa-miR-4283 750-756 -0.278 92.0 -0.154 85.0 noncon
hsa-miR-6759-3p 600-607 -0.277 95.0 -0.154 90.0 noncon
hsa-miR-7111-3p 1489-1496 -0.276 96.0 -0.137 89.0 noncon
hsa-miR-200b-3p 100-106 -0.276 98.0 -0.276 98.0 con
hsa-miR-200c-3p 100-106 -0.276 98.0 -0.276 98.0 con
hsa-miR-429 100-106 -0.276 98.0 -0.276 98.0 con
hsa-miR-4525 1381-1388 -0.275 96.0 -0.137 87.0 noncon
hsa-miR-6857-5p 545-551 -0.275 96.0 -0.153 91.0 noncon
hsa-miR-6832-3p 4721-4728 -0.273 96.0 -0.052 77.0 noncon
hsa-miR-7854-3p 3097-3104 -0.272 96.0 -0.052 64.0 noncon
hsa-miR-5586-3p 1632-1638 -0.272 96.0 -0.135 89.0 noncon
hsa-miR-656-3p 35-42 -0.272 99.0 -0.272 99.0 noncon
hsa-miR-5572 1876-1883 -0.27 96.0 -0.134 90.0 noncon
hsa-miR-520d-3p 1070-1077 -0.263 96.0 -0.146 91.0 noncon
hsa-miR-520a-3p 1070-1077 -0.263 96.0 -0.146 91.0 noncon
hsa-miR-629-5p 1561-1568 -0.26 96.0 -0.129 89.0 noncon
hsa-miR-3910 3010-3017 -0.257 97.0 -0.05 78.0 noncon
hsa-miR-372-3p 1070-1077 -0.255 95.0 -0.142 90.0 noncon
hsa-miR-520c-3p 1070-1077 -0.255 96.0 -0.142 91.0 noncon
hsa-miR-520e 1070-1077 -0.255 96.0 -0.142 91.0 noncon
hsa-miR-520b 1070-1077 -0.255 96.0 -0.142 91.0 noncon
hsa-miR-8089 4070-4076 -0.254 94.0 -0.049 63.0 noncon
hsa-miR-4646-5p 412-418 -0.254 97.0 -0.162 94.0 noncon
hsa-miR-384 1320-1327 -0.254 97.0 -0.141 92.0 noncon
hsa-miR-4662b 146-153 -0.254 97.0 -0.254 98.0 noncon
hsa-miR-6883-5p 1258-1265 -0.253 94.0 -0.141 84.0 noncon
hsa-miR-149-3p 1258-1265 -0.253 93.0 -0.141 82.0 noncon
hsa-miR-5584-5p 649-655 -0.252 97.0 -0.14 91.0 noncon
hsa-miR-4308 671-678 -0.252 96.0 -0.14 90.0 noncon
hsa-miR-487a-5p 158-165 -0.252 97.0 -0.252 98.0 noncon
hsa-miR-377-5p 4252-4259 -0.25 94.0 -0.048 68.0 noncon