GlyClass

GlycoEnzDB
Comprehensive glycoEnzyme database and dynamic pathway generation

Description: The GlycoEnzDB is a manually curated glycoEnzyme database, primarily focused on humans. It covers 390 enzymes across 28 pathway maps. Facilities are also available to create custom glycosylation reaction pathways using experimental data in SBML format and for pathway simulation
Contacts: Sriram Neelamegham (neel@buffalo.edu), Yusen Zhou (yusenzho@buffalo.edu) or Ted Groth (tgroth@buffalo.edu).

GlycoEnzDB
Create glycosylation pathway
Custom database generation
Instructions/Reference
 
Pathway Map
HS3ST3B1



General Information
Gene Symbol:   
HS3ST3B1
Protein name:   
Heparan sulfate glucosamine 3-O-sulfotransferase 3B1
Enzyme Class:   
Sulfate synthase
CAZy Family:   
E.C. No:   
2.8.2.30
Organs:   
Reaction (SNFG):   
PAPS +
→
+ PAP
PAPS +
→
+ PAP
Reaction (IUBMB):   
No specified reaction.
About

• Sulfation of heparan sulfate occurs at C2 of HexA. It also occurs at C3, C6, and NS residues of GlcNAc or GlcNS. HSPGs are major regulators of cell behavior with roles in inflammation, blood coagulation, tumor invasion, and malignancy.
• Seven isoenzymes display HS 3-O-sulfotransferase activity, with all enzymes transferring sulfate to C3 of GlcNAc in heparin and heparan sulfate.
• HS3ST1 sulfates an N-sulfo glucosamine (GlcNS) that has a nonsulfated glucuronic acid (GlcA) or an iduronic acid (IdoA) at the nonreducing end. This modification is necessary for anti-thrombin activity of heparin. HS
• HS3ST-2, 3 (3A and 3B), 4, and 6, all sulfate a GlcNS6S residue that has a 2-O-sulfated iduronic acid (IdoA2S) on the nonreducing end. This does not provide anti-thrombin activity, but serves viral entry for herpes simplex virus type-1.
• The tissue distribution of the different HS3STs is quite different.

Related Pathway
KEGG ORTHOLOGY:   
K07809
KEGG Pathway:   
ko00534 
Reactome:   
R-HSA-2022928
Related Database
Organism:   
Homo sapiens
Uniprot:   
Q9Y662 
NCBI GeneID:   
9953
GlyMap GeneID:   
9953
DNA aRefSeq:   
NM_006041.2 
Protein RefSeq:   
NP_006032.1 
BRENDA:   
Kinetic info.
OMIM number:   
604058
GeneCards database:   
HS3ST3B1
Enzyme Specificity
Glycan Type:  
     GAG
Compartment:  
     Cis, Medial, Trans, TGN
Substrate:  
     IdoA(a1-4 [2S])GlcN(a1-4 [2S]) or IdoA(a1-4 [2S])GlcN(a1-4 [2,6S])
Product:  
     IdoA(a1-4 [2S])GlcN(a1-4 [2,3S]) or IdoA(a1-4 [2S])GlcN(a1-4 [2,3,6S])
Simulation Parameters
Mechaelis-Menten parameters (suggested)
VM:       (pmol/cell • hr)       KM:       (pM)
Relative conc of enzymes in compartment.(sum will be normalized to 1)
ER Cis- Medial- Trans- TGN










Expression Data in Tissue {ref. 5} Source from NCBI gene database
adrenal appendix bone marrow brain colon duodenum endometrium esophagus fat gall bladder heart kidney liver lung lymph node ovary pancreas placenta prostate salivary gland skin small intestine spleen stomach testis thyroid urinary bladder 8 6 4 2 0 RPKM
Expression Data in Cells {ref. 13} Source from Cancer Cell Line Encyclopedia database
Kidney Lung Breast Prostate Large intestine Ovary HL60 haematopoietic and lymphoid tissue Skin Central nervous system 786O A498 ACHN CAKI1 UO31 A549 EKVX HOP62 HOP92 NCIH226 NCIH23 NCIH322 NCIH460 NCIH522 SHP77 BT549 HS578T MCF7 MDAMB231 MDAMB468 T47D DU145 PC3 HCT116 HCT15 HT29 KM12 SW620 OVCAR4 OVCAR8 IGROV1 SKOV3 HL60 K562 RPMI8226 LOXIMVI MALME3M RPMI7951 SKMEL28 SKMEL5 UACC257 UACC62 SF268 SF295 SF539 SNB75 30 20 10 0 Transcripts per million (TPM)
Transcription factor {ref. 10}
No high degree of confidence data
miRNA-GlycoEnzyme Interactome {ref. 9}
miRNA Name Pos. Context Score Frac. Wt. Context Frac. Conserved Quality
hsa-miR-99a-5p 297-304 -0.579 98.0 -0.579 99.0 con
hsa-miR-100-5p 297-304 -0.571 98.0 -0.571 99.0 con
hsa-miR-99b-5p 297-304 -0.566 98.0 -0.566 99.0 con
hsa-miR-6859-3p 3299-3306 -0.447 98.0 -0.441 98.0 noncon
hsa-miR-5193 3262-3269 -0.439 98.0 -0.433 98.0 noncon
hsa-miR-210-5p 1706-1713 -0.402 98.0 -0.402 98.0 noncon
hsa-miR-4529-5p 3468-3475 -0.398 96.0 -0.392 97.0 noncon
hsa-miR-613 171-178 -0.386 98.0 -0.386 98.0 con
hsa-miR-4804-5p 487-494 -0.383 92.0 -0.383 95.0 noncon
hsa-miR-1-3p 171-178 -0.376 98.0 -0.376 98.0 con
hsa-miR-6871-3p 2285-2292 -0.37 98.0 -0.37 98.0 noncon
hsa-miR-206 171-178 -0.368 97.0 -0.368 98.0 con
hsa-miR-302f 109-116 -0.363 98.0 -0.363 99.0 noncon
hsa-miR-6499-5p 894-900 -0.355 90.0 -0.355 93.0 noncon
hsa-miR-539-3p 3729-3736 -0.35 96.0 -0.344 97.0 noncon
hsa-miR-7114-3p 2651-2658 -0.35 96.0 -0.35 97.0 noncon
hsa-miR-218-5p 272-279 -0.34 97.0 -0.34 97.0 con
hsa-miR-6069 130-137 -0.337 96.0 -0.337 97.0 noncon
hsa-miR-29a-3p 221-227 -0.337 94.0 -0.337 96.0 con
hsa-miR-29c-3p 221-227 -0.337 94.0 -0.337 96.0 con
hsa-miR-29b-3p 221-227 -0.337 94.0 -0.337 96.0 con
hsa-miR-182-5p 3752-3758 -0.324 96.0 -0.001 20.0 noncon
hsa-miR-6759-3p 3107-3114 -0.32 96.0 -0.32 97.0 noncon
hsa-miR-4767 895-901 -0.319 90.0 -0.319 92.0 noncon
hsa-miR-4650-3p 1785-1792 -0.314 94.0 -0.314 96.0 noncon
hsa-miR-323a-5p 2931-2938 -0.307 95.0 -0.307 96.0 noncon
hsa-miR-6732-5p 2968-2974 -0.305 94.0 -0.305 95.0 noncon
hsa-miR-555 20-27 -0.3 93.0 -0.3 96.0 noncon
hsa-miR-200c-5p 2739-2746 -0.299 96.0 -0.299 97.0 noncon
hsa-miR-3187-3p 3469-3476 -0.297 98.0 -0.293 98.0 noncon
hsa-miR-644a 134-140 -0.296 94.0 -0.296 96.0 noncon
hsa-miR-6746-5p 828-834 -0.29 92.0 -0.29 95.0 noncon
hsa-miR-4664-5p 2845-2852 -0.289 94.0 -0.289 95.0 noncon
hsa-miR-1913 1708-1714 -0.284 95.0 -0.284 96.0 noncon
hsa-miR-4704-3p 3229-3235 -0.283 96.0 -0.283 97.0 noncon
hsa-miR-1180-5p 2651-2658 -0.282 97.0 -0.282 97.0 noncon
hsa-miR-493-5p 3618-3625 -0.276 97.0 -0.271 98.0 con
hsa-miR-3611 209-216 -0.274 97.0 -0.274 98.0 noncon
hsa-miR-324-3p 1708-1714 -0.274 95.0 -0.274 96.0 noncon
hsa-miR-3145-5p 3048-3055 -0.273 97.0 -0.273 98.0 noncon
hsa-miR-5682 2286-2293 -0.272 95.0 -0.272 96.0 noncon
hsa-miR-6773-3p 3313-3320 -0.272 95.0 -0.268 96.0 noncon
hsa-miR-550a-3p 2739-2746 -0.27 95.0 -0.27 97.0 noncon
hsa-miR-6762-3p 2069-2076 -0.266 93.0 -0.266 95.0 noncon
hsa-miR-655-5p 1758-1765 -0.265 95.0 -0.265 96.0 noncon
hsa-miR-409-5p 1658-1665 -0.26 90.0 -0.26 94.0 noncon
hsa-miR-342-5p 2845-2852 -0.259 92.0 -0.259 94.0 noncon
hsa-miR-3665 2274-2281 -0.259 92.0 -0.259 94.0 noncon
hsa-miR-5589-3p 2761-2768 -0.256 95.0 -0.256 96.0 noncon
hsa-miR-3913-3p 1983-1989 -0.255 92.0 -0.255 94.0 noncon
hsa-miR-383-5p.1 2420-2427 -0.255 91.0 -0.255 94.0 noncon
hsa-miR-221-5p 3357-3364 -0.254 96.0 -0.251 97.0 noncon
hsa-miR-8073 3357-3364 -0.254 96.0 -0.251 97.0 noncon
hsa-miR-3606-5p 118-125 -0.25 93.0 -0.25 95.0 noncon