GlyClass

GlycoEnzDB
Comprehensive glycoEnzyme database and dynamic pathway generation

Description: The GlycoEnzDB is a manually curated glycoEnzyme database, primarily focused on humans. It covers 390 enzymes across 28 pathway maps. Facilities are also available to create custom glycosylation reaction pathways using experimental data in SBML format and for pathway simulation
Contacts: Sriram Neelamegham (neel@buffalo.edu), Yusen Zhou (yusenzho@buffalo.edu) or Ted Groth (tgroth@buffalo.edu).

GlycoEnzDB
Create glycosylation pathway
Custom database generation
Instructions/Reference
 
Pathway Map
HS3ST4



General Information
Gene Symbol:   
HS3ST4
Protein name:   
Heparan sulfate glucosamine 3-O-sulfotransferase 4
Enzyme Class:   
Sulfate synthase
CAZy Family:   
E.C. No:   
2.8.2.23
Organs:   
Reaction (SNFG):   
PAPS +
→
+ PAP
PAPS +
→
+ PAP
Reaction (IUBMB):   
No specified reaction.
About

• Sulfation of heparan sulfate occurs at C2 of HexA. It also occurs at C3, C6, and NS residues of GlcNAc or GlcNS. HSPGs are major regulators of cell behavior with roles in inflammation, blood coagulation, tumor invasion, and malignancy.
• Seven isoenzymes display HS 3-O-sulfotransferase activity, with all enzymes transferring sulfate to C3 of GlcNAc in heparin and heparan sulfate.
• HS3ST1 sulfates an N-sulfo glucosamine (GlcNS) that has a nonsulfated glucuronic acid (GlcA) or an iduronic acid (IdoA) at the nonreducing end. This modification is necessary for anti-thrombin activity of heparin. HS
• HS3ST-2, 3 (3A and 3B), 4, and 6, all sulfate a GlcNS6S residue that has a 2-O-sulfated iduronic acid (IdoA2S) on the nonreducing end. This does not provide anti-thrombin activity, but serves viral entry for herpes simplex virus type-1.
• The tissue distribution of the different HS3STs is quite different.

Related Pathway
KEGG ORTHOLOGY:   
K09678
KEGG Pathway:   
No Record 
Reactome:   
R-HSA-2022928
Related Database
Organism:   
Homo sapiens
Uniprot:   
Q9Y661 
NCBI GeneID:   
9951
GlyMap GeneID:   
9951
DNA aRefSeq:   
NM_006040.2 
Protein RefSeq:   
NP_006031.2 
BRENDA:   
Kinetic info.
OMIM number:   
604059
GeneCards database:   
HS3ST4
Enzyme Specificity
Glycan Type:  
     GAG
Compartment:  
     Cis, Medial, Trans, TGN
Substrate:  
     IdoA(a1-4 [2S])GlcN(a1-4 [2S]) or IdoA(a1-4 [2S])GlcN(a1-4 [2,6S])
Product:  
     IdoA(a1-4 [2S])GlcN(a1-4 [2,3S]) or IdoA(a1-4 [2S])GlcN(a1-4 [2,3,6S])
Simulation Parameters
Mechaelis-Menten parameters (suggested)
VM:       (pmol/cell • hr)       KM:       (pM)
Relative conc of enzymes in compartment.(sum will be normalized to 1)
ER Cis- Medial- Trans- TGN










Expression Data in Tissue {ref. 5} Source from NCBI gene database
adrenal appendix bone marrow brain colon duodenum endometrium esophagus fat gall bladder heart kidney liver lung lymph node ovary pancreas placenta prostate salivary gland skin small intestine spleen stomach testis thyroid urinary bladder 10 8 6 4 2 0 RPKM
Expression Data in Cells {ref. 13} Source from Cancer Cell Line Encyclopedia database
Kidney Lung Breast Prostate Large intestine Ovary HL60 haematopoietic and lymphoid tissue Skin Central nervous system 786O A498 ACHN CAKI1 UO31 A549 EKVX HOP62 HOP92 NCIH226 NCIH23 NCIH322 NCIH460 NCIH522 SHP77 BT549 HS578T MCF7 MDAMB231 MDAMB468 T47D DU145 PC3 HCT116 HCT15 HT29 KM12 SW620 OVCAR4 OVCAR8 IGROV1 SKOV3 HL60 K562 RPMI8226 LOXIMVI MALME3M RPMI7951 SKMEL28 SKMEL5 UACC257 UACC62 SF268 SF295 SF539 SNB75 2 0 Transcripts per million (TPM)
Transcription factor {ref. 10}
Transcriptional start site (TSS) 0 200 -2000 -1800 -1600 -1400 -1200 -1000 -800 -600 -400 -200
ENST00000331351 MYC MYCN MAZ AR POU5F1
miRNA-GlycoEnzyme Interactome {ref. 9}
miRNA Name Pos. Context Score Frac. Wt. Context Frac. Conserved Quality
hsa-miR-6798-3p 859-866 -0.538 98.0 -0.538 98.0 noncon
hsa-miR-4455 623-630 -0.469 97.0 -0.469 98.0 noncon
hsa-miR-6766-5p 627-634 -0.429 97.0 -0.429 98.0 noncon
hsa-miR-4683 1298-1305 -0.42 99.0 -0.42 99.0 noncon
hsa-miR-6756-5p 627-634 -0.41 97.0 -0.41 97.0 noncon
hsa-miR-4454 769-776 -0.385 98.0 -0.385 99.0 noncon
hsa-miR-6746-5p 1389-1395 -0.376 97.0 -0.376 98.0 noncon
hsa-miR-942-3p 1031-1038 -0.37 98.0 -0.37 99.0 noncon
hsa-miR-6788-3p 1134-1140 -0.368 93.0 -0.368 95.0 noncon
hsa-miR-4713-3p 167-174 -0.365 94.0 -0.365 95.0 noncon
hsa-miR-769-5p 538-545 -0.363 98.0 -0.363 99.0 noncon
hsa-miR-549a 300-307 -0.363 97.0 -0.363 98.0 noncon
hsa-miR-5195-3p 1328-1335 -0.357 96.0 -0.357 97.0 noncon
hsa-miR-6731-5p 917-924 -0.351 97.0 -0.351 98.0 noncon
hsa-miR-3614-3p 17-24 -0.35 98.0 -0.35 98.0 noncon
hsa-miR-8085 917-924 -0.343 97.0 -0.343 97.0 noncon
hsa-miR-1270 1299-1306 -0.341 98.0 -0.341 99.0 noncon
hsa-miR-4529-5p 229-236 -0.338 95.0 -0.338 96.0 noncon
hsa-miR-6825-5p 568-575 -0.334 92.0 -0.334 94.0 noncon
hsa-miR-6790-3p 815-822 -0.325 97.0 -0.325 98.0 noncon
hsa-miR-302c-3p.2 1283-1289 -0.324 97.0 -0.324 98.0 con
hsa-miR-620 1299-1306 -0.322 98.0 -0.322 98.0 noncon
hsa-miR-4641 414-421 -0.322 94.0 -0.322 96.0 noncon
hsa-miR-593-3p 312-319 -0.306 97.0 -0.306 97.0 noncon
hsa-miR-4297 16-22 -0.305 94.0 -0.305 95.0 noncon
hsa-miR-4764-3p 1352-1359 -0.299 96.0 -0.299 97.0 noncon
hsa-miR-6886-3p 980-987 -0.295 93.0 -0.295 95.0 noncon
hsa-miR-6748-5p 629-636 -0.29 91.0 -0.29 93.0 noncon
hsa-miR-302a-3p 1284-1290 -0.288 97.0 -0.288 98.0 con
hsa-miR-302b-3p 1284-1290 -0.288 97.0 -0.288 98.0 con
hsa-miR-302c-3p.1 1284-1290 -0.288 97.0 -0.288 98.0 con
hsa-miR-302d-3p 1284-1290 -0.288 97.0 -0.288 98.0 con
hsa-miR-650 134-141 -0.288 96.0 -0.288 97.0 noncon
hsa-miR-6764-3p 991-998 -0.287 97.0 -0.287 98.0 noncon
hsa-miR-491-5p 570-577 -0.286 94.0 -0.286 96.0 noncon
hsa-miR-4747-5p 1239-1246 -0.281 95.0 -0.281 96.0 noncon
hsa-miR-3612 134-141 -0.28 95.0 -0.28 97.0 noncon
hsa-miR-520f-3p 1283-1289 -0.28 95.0 -0.28 97.0 con
hsa-miR-145-5p 1328-1335 -0.279 95.0 -0.279 96.0 noncon
hsa-miR-6796-5p 571-577 -0.279 92.0 -0.279 94.0 noncon
hsa-miR-6824-3p 991-998 -0.279 97.0 -0.279 98.0 noncon
hsa-miR-4654 545-552 -0.278 96.0 -0.278 97.0 noncon
hsa-miR-4731-5p 66-72 -0.277 96.0 -0.277 96.0 noncon
hsa-miR-92a-2-5p 1074-1081 -0.275 96.0 -0.275 96.0 noncon
hsa-miR-302e 1284-1290 -0.266 96.0 -0.266 97.0 con
hsa-miR-5196-5p 1239-1246 -0.265 94.0 -0.265 95.0 noncon
hsa-miR-4309 1148-1154 -0.265 95.0 -0.265 96.0 noncon
hsa-miR-4276 605-612 -0.263 95.0 -0.263 97.0 noncon
hsa-miR-21-5p 29-36 -0.262 95.0 -0.262 97.0 noncon
hsa-miR-4639-3p 1122-1129 -0.258 96.0 -0.258 97.0 noncon
hsa-miR-4769-3p 1116-1123 -0.257 97.0 -0.257 98.0 noncon
hsa-miR-4683 105-111 -0.257 95.0 -0.257 96.0 noncon
hsa-miR-6817-5p 1116-1123 -0.257 96.0 -0.257 97.0 noncon
hsa-miR-5189-3p 832-839 -0.256 92.0 -0.256 95.0 noncon
hsa-miR-194-3p 243-249 -0.255 95.0 -0.255 97.0 noncon
hsa-miR-301a-5p 310-317 -0.253 98.0 -0.253 98.0 noncon
hsa-miR-4475 1236-1242 -0.253 96.0 -0.253 97.0 noncon
hsa-miR-561-5p 56-63 -0.252 94.0 -0.252 96.0 noncon
hsa-miR-5088-5p 691-697 -0.251 93.0 -0.251 95.0 noncon
hsa-miR-5581-5p 16-22 -0.251 90.0 -0.251 93.0 noncon
hsa-miR-3151-5p 1073-1079 -0.25 93.0 -0.25 95.0 noncon