GlyClass

GlycoEnzDB
Comprehensive glycoEnzyme database and dynamic pathway generation

Description: The GlycoEnzDB is a manually curated glycoEnzyme database, primarily focused on humans. It covers 390 enzymes across 28 pathway maps. Facilities are also available to create custom glycosylation reaction pathways using experimental data in SBML format and for pathway simulation
Contacts: Sriram Neelamegham (neel@buffalo.edu), Yusen Zhou (yusenzho@buffalo.edu) or Ted Groth (tgroth@buffalo.edu).

GlycoEnzDB
Create glycosylation pathway
Custom database generation
Instructions/Reference
 
Pathway Map
NAGLU



General Information
Gene Symbol:   
NAGLU
Protein name:   
Alpha-N-acetylglucosaminidase
Enzyme Class:   
Glycosidase
CAZy Family:   
GH89
E.C. No:   
3.2.1.50
Tissues:   
Muscular, Epithelial, Connective, Nervous
Organs:   
Reaction (SNFG):   
NAGLU
H2O +
→
+
Reaction (IUBMB):   
No specified reaction.
About

• N-acetylglucosaminidase hydrolyzes GlcNAc from glycans in lysosomes. Involved in the degradation of heparan sulfates.
• Deficiencies in NAGLU causes Sanfilippo disease, also called mucopolysaccharosis type III. This disease is characterized by congitive declilne throughout and hyperactivity. Mutations in NAGLU causes death between 20 and 30 years of age.

Related Pathway
KEGG ORTHOLOGY:   
KEGG Pathway:   
No Record 
Reactome:   
R-HSA-2024096
Related Database
Organism:   
Homo sapiens
Uniprot:   
P54802 
NCBI GeneID:   
4669
GlyMap GeneID:   
4669
DNA aRefSeq:   
NM_000263.3 
Protein RefSeq:   
NP_000254.2 
BRENDA:   
Kinetic info.
OMIM number:   
609701
GeneCards database:   
NAGLU
Enzyme Specificity
Glycan Type:  
     GAG
Compartment:  
     
Substrate:  
     GlcNAc(a1-4)GlcA(b1-4)
Product:  
     GlcA(b1-4)
Constraints:  
     None
Simulation Parameters
Mechaelis-Menten parameters (suggested)
VM:       (pmol/cell • hr)       KM:       (pM)
Relative conc of enzymes in compartment.(sum will be normalized to 1)
ER Cis- Medial- Trans- TGN










Expression Data in Tissue {ref. 5} Source from NCBI gene database
adrenal appendix bone marrow brain colon duodenum endometrium esophagus fat gall bladder heart kidney liver lung lymph node ovary pancreas placenta prostate salivary gland skin small intestine spleen stomach testis thyroid urinary bladder 12 10 8 6 4 2 0 RPKM
Expression Data in Cells {ref. 13} Source from Cancer Cell Line Encyclopedia database
Kidney Lung Breast Prostate Large intestine Ovary HL60 haematopoietic and lymphoid tissue Skin Central nervous system 786O A498 ACHN CAKI1 UO31 A549 EKVX HOP62 HOP92 NCIH226 NCIH23 NCIH322 NCIH460 NCIH522 SHP77 BT549 HS578T MCF7 MDAMB231 MDAMB468 T47D DU145 PC3 HCT116 HCT15 HT29 KM12 SW620 OVCAR4 OVCAR8 IGROV1 SKOV3 HL60 K562 RPMI8226 LOXIMVI MALME3M RPMI7951 SKMEL28 SKMEL5 UACC257 UACC62 SF268 SF295 SF539 SNB75 120 100 80 60 40 20 0 Transcripts per million (TPM)
Transcription factor {ref. 10}
No high degree of confidence data
miRNA-GlycoEnzyme Interactome {ref. 9}
miRNA Name Pos. Context Score Frac. Wt. Context Frac. Conserved Quality
hsa-miR-18a-3p 40-47 -0.449 99.0 -0.449 99.0 noncon
hsa-miR-101-3p.1 189-195 -0.448 99.0 -0.448 99.0 noncon
hsa-miR-4321 29-35 -0.447 92.0 -0.447 94.0 noncon
hsa-miR-6775-3p 51-57 -0.369 96.0 -0.369 97.0 noncon
hsa-miR-7846-3p 28-34 -0.365 92.0 -0.365 94.0 noncon
hsa-miR-1293 166-173 -0.356 98.0 -0.356 98.0 noncon
hsa-miR-4267 60-67 -0.346 98.0 -0.346 98.0 noncon
hsa-miR-6880-5p 159-166 -0.338 95.0 -0.338 96.0 noncon
hsa-miR-6773-5p 53-59 -0.311 94.0 -0.311 96.0 noncon
hsa-miR-1291 51-57 -0.302 93.0 -0.302 95.0 noncon
hsa-miR-6724-5p 53-59 -0.297 94.0 -0.297 96.0 noncon
hsa-miR-188-3p 177-183 -0.29 93.0 -0.29 95.0 noncon
hsa-miR-4483 166-173 -0.272 97.0 -0.272 98.0 noncon
hsa-miR-4667-3p 141-147 -0.261 95.0 -0.261 96.0 noncon
hsa-miR-661 85-91 -0.258 95.0 -0.258 96.0 noncon
hsa-miR-3922-5p 18-24 -0.258 94.0 -0.258 96.0 noncon
hsa-miR-874-3p 39-45 -0.251 94.0 -0.251 96.0 noncon