GlyClass

GlycoEnzDB
Comprehensive glycoEnzyme database and dynamic pathway generation

Description: The GlycoEnzDB is a manually curated glycoEnzyme database, primarily focused on humans. It covers 390 enzymes across 28 pathway maps. Facilities are also available to create custom glycosylation reaction pathways using experimental data in SBML format and for pathway simulation
Contacts: Sriram Neelamegham (neel@buffalo.edu), Yusen Zhou (yusenzho@buffalo.edu) or Ted Groth (tgroth@buffalo.edu).

GlycoEnzDB
Create glycosylation pathway
Custom database generation
Instructions/Reference
 
Pathway Map
UST



General Information
Gene Symbol:   
UST
Protein name:   
Uronyl 2-sulfotransferase
Enzyme Class:   
Sulfate synthase
CAZy Family:   
E.C. No:   
2.8.2.*
Organs:   
Reaction (SNFG):   
PAPS +
→
+ PAP
PAPS +
→
+ PAP
Reaction (IUBMB):   
No specified reaction.
About

• Chondroitin sulfate chains are composed of GlcAβ1-3GalNAcβ1-4 repeating unit. Various sulfotransferases, CHST3, 7, 11, 12, 13, 14, 15, and UST, sulfate either GalNAc and GlcA residues. CS are a major functional component of aggrecan, and needed for cartilage formation and maintenance.
• This is also called CS2ST. It transfers sulfo group to C2 of GlcA or IdoA in the context of GalNAc.
• IdoA2S residue present in a highly sulfated chondroitin sulfate B (dermatan sulfate) hexasaccharide
• Does not have activity on HS

Related Pathway
KEGG ORTHOLOGY:   
K03193
KEGG Pathway:   
ko00532 
Reactome:   
R-HSA-2022923
Related Database
Organism:   
Homo sapiens
Uniprot:   
Q9Y2C2 
NCBI GeneID:   
10090
GlyMap GeneID:   
10090
DNA aRefSeq:   
NM_005715.2 
Protein RefSeq:   
NP_005706.1 
BRENDA:   
Kinetic info.
OMIM number:   
610752
GeneCards database:   
UST
Enzyme Specificity
Glycan Type:  
     GAG
Compartment:  
     
Substrate:  
     GalNAc(b1-4)GlcA(b1-3)GalNAc(b1-?) or GalNAc(b1-4)IdoA(a1-3)GalNAc(b1-?)
Product:  
     GalNAc(b1-4)GlcA(b1-3 [2S])GalNAc(b1-?) or GalNAc(b1-4)IdoA(a1-3 [2S])GalNAc(b1-?)
Constraints:  
     var=50%
Simulation Parameters
Mechaelis-Menten parameters (suggested)
VM:       (pmol/cell • hr)       KM:       (pM)
Relative conc of enzymes in compartment.(sum will be normalized to 1)
ER Cis- Medial- Trans- TGN










Expression Data in Tissue {ref. 5} Source from NCBI gene database
adrenal appendix bone marrow brain colon duodenum endometrium esophagus fat gall bladder heart kidney liver lung lymph node ovary pancreas placenta prostate salivary gland skin small intestine spleen stomach testis thyroid urinary bladder 20 10 0 RPKM
Expression Data in Cells {ref. 13} Source from Cancer Cell Line Encyclopedia database
Kidney Lung Breast Prostate Large intestine Ovary HL60 haematopoietic and lymphoid tissue Skin Central nervous system 786O A498 ACHN CAKI1 UO31 A549 EKVX HOP62 HOP92 NCIH226 NCIH23 NCIH322 NCIH460 NCIH522 SHP77 BT549 HS578T MCF7 MDAMB231 MDAMB468 T47D DU145 PC3 HCT116 HCT15 HT29 KM12 SW620 OVCAR4 OVCAR8 IGROV1 SKOV3 HL60 K562 RPMI8226 LOXIMVI MALME3M RPMI7951 SKMEL28 SKMEL5 UACC257 UACC62 SF268 SF295 SF539 SNB75 30 20 10 0 Transcripts per million (TPM)
Transcription factor {ref. 10}
Transcriptional start site (TSS) 0 200 -2000 -1800 -1600 -1400 -1200 -1000 -800 -600 -400 -200
ENST00000367463 ZNF263 NFATC3 TAF1 ARNT SMAD4 MYC IRF4 RBPJ ZNF554 NFKB2 TEAD4 TP53 NFKB1 UBTF KLF15 SP4 TCF4 TCF4 HSF1 SP2 GATA3 SP3 SP3 ZNF467 ZSCAN22 FEZF1 FEZF1 FEZF1 MZF1 PRDM1 SP1 OSR2 OSR2 YY1 FOXP1 BHLHE40 GATA6 GATA6 GATA6 SP7 DEAF1 SIX5 USF2 USF2 EGR3 IKZF2 RELB RELB ZNF341 EGR2 EGR2 SMAD2 MAZ MAZ VEZF1 NFAT5 PATZ1 ZNF507 NFE2 ZBTB7A EGR1 ZEB2 ZNF629
miRNA-GlycoEnzyme Interactome {ref. 9}
miRNA Name Pos. Context Score Frac. Wt. Context Frac. Conserved Quality
hsa-miR-584-3p 2828-2835 -0.536 99.0 -0.337 98.0 noncon
hsa-miR-2114-5p 74-81 -0.494 99.0 -0.474 99.0 noncon
hsa-miR-6813-5p 301-308 -0.48 99.0 -0.451 99.0 noncon
hsa-miR-6085 301-308 -0.413 98.0 -0.389 98.0 noncon
hsa-miR-1911-3p 2135-2142 -0.39 98.0 -0.367 98.0 noncon
hsa-miR-4800-3p 2390-2396 -0.385 92.0 -0.248 86.0 noncon
hsa-miR-6724-5p 2506-2513 -0.383 97.0 -0.247 93.0 noncon
hsa-miR-206 2621-2628 -0.369 97.0 -0.238 94.0 con
hsa-miR-1-3p 2621-2628 -0.369 97.0 -0.238 94.0 con
hsa-miR-613 2621-2628 -0.363 97.0 -0.234 94.0 con
hsa-miR-6773-5p 2506-2513 -0.362 97.0 -0.234 92.0 noncon
hsa-miR-7160-3p 2054-2061 -0.362 97.0 -0.341 97.0 noncon
hsa-miR-6865-5p 1467-1474 -0.36 97.0 -0.339 98.0 noncon
hsa-miR-1912 2313-2320 -0.355 99.0 -0.229 97.0 noncon
hsa-miR-6815-5p 1467-1474 -0.352 97.0 -0.331 98.0 noncon
hsa-miR-218-1-3p 1031-1038 -0.351 96.0 -0.331 97.0 noncon
hsa-miR-6741-5p 981-988 -0.347 99.0 -0.327 98.0 noncon
hsa-miR-6509-3p 387-394 -0.343 97.0 -0.323 98.0 noncon
hsa-miR-4445-3p 2738-2744 -0.34 93.0 -0.219 88.0 noncon
hsa-miR-608 267-274 -0.337 90.0 -0.317 91.0 noncon
hsa-miR-1295b-5p 2313-2320 -0.336 98.0 -0.218 97.0 noncon
hsa-miR-4505 2426-2433 -0.333 96.0 -0.216 91.0 noncon
hsa-miR-6716-5p 115-122 -0.326 96.0 -0.307 97.0 noncon
hsa-miR-181a-2-3p 386-393 -0.326 98.0 -0.307 98.0 noncon
hsa-miR-1291 2404-2410 -0.325 94.0 -0.211 89.0 noncon
hsa-miR-6739-3p 106-113 -0.323 97.0 -0.311 97.0 noncon
hsa-miR-92a-1-5p 772-779 -0.323 93.0 -0.304 94.0 noncon
hsa-miR-613 114-120 -0.321 96.0 -0.308 97.0 con
hsa-miR-6755-3p 2484-2491 -0.319 94.0 -0.207 87.0 noncon
hsa-miR-4764-3p 2649-2656 -0.316 96.0 -0.205 94.0 noncon
hsa-miR-5193 2017-2024 -0.314 95.0 -0.296 95.0 noncon
hsa-miR-4433b-5p 2265-2272 -0.311 95.0 -0.202 90.0 noncon
hsa-miR-526a 1770-1777 -0.307 97.0 -0.289 97.0 noncon
hsa-miR-518d-5p 1770-1777 -0.307 97.0 -0.289 97.0 noncon
hsa-miR-520c-5p 1770-1777 -0.307 97.0 -0.289 97.0 noncon
hsa-miR-518f-5p 1770-1777 -0.307 97.0 -0.289 97.0 noncon
hsa-miR-504-5p.1 415-422 -0.306 98.0 -0.289 98.0 noncon
hsa-miR-625-5p 262-269 -0.303 91.0 -0.286 92.0 noncon
hsa-miR-1200 2641-2648 -0.302 97.0 -0.197 94.0 noncon
hsa-miR-8076 2167-2174 -0.297 97.0 -0.28 97.0 noncon
hsa-miR-30c-2-3p 1001-1008 -0.296 98.0 -0.279 98.0 noncon
hsa-miR-30c-1-3p 1001-1008 -0.296 98.0 -0.279 98.0 noncon
hsa-miR-5585-5p 2520-2527 -0.295 96.0 -0.192 94.0 noncon
hsa-miR-875-3p 35-42 -0.293 98.0 -0.293 98.0 noncon
hsa-miR-6775-3p 2404-2410 -0.293 92.0 -0.191 85.0 noncon
hsa-miR-3611 2371-2378 -0.292 98.0 -0.19 96.0 noncon
hsa-miR-523-5p 1770-1777 -0.291 97.0 -0.274 97.0 noncon
hsa-miR-519c-5p 1770-1777 -0.291 97.0 -0.274 97.0 noncon
hsa-miR-519a-5p 1770-1777 -0.291 97.0 -0.274 97.0 noncon
hsa-miR-522-5p 1770-1777 -0.291 97.0 -0.274 97.0 noncon
hsa-miR-518e-5p 1770-1777 -0.291 97.0 -0.274 97.0 noncon
hsa-miR-519b-5p 1770-1777 -0.291 97.0 -0.274 97.0 noncon
hsa-miR-6750-5p 1170-1177 -0.29 98.0 -0.273 98.0 noncon
hsa-miR-6822-5p 1170-1177 -0.29 98.0 -0.273 98.0 noncon
hsa-miR-4313 707-714 -0.29 92.0 -0.273 93.0 noncon
hsa-miR-1299 36-43 -0.289 97.0 -0.289 97.0 noncon
hsa-miR-6730-5p 2611-2618 -0.283 98.0 -0.185 96.0 noncon
hsa-miR-541-5p 66-73 -0.281 97.0 -0.281 98.0 noncon
hsa-miR-5588-3p 75-81 -0.281 95.0 -0.271 96.0 noncon
hsa-miR-324-3p 1984-1991 -0.28 95.0 -0.264 96.0 noncon
hsa-miR-4677-5p 105-112 -0.277 97.0 -0.267 98.0 noncon
hsa-miR-6890-5p 269-276 -0.273 95.0 -0.257 96.0 noncon
hsa-miR-4795-5p 403-410 -0.272 95.0 -0.257 95.0 noncon
hsa-miR-4425 1003-1010 -0.27 90.0 -0.255 92.0 noncon
hsa-miR-505-5p 226-233 -0.27 96.0 -0.255 96.0 noncon
hsa-miR-6842-3p 2427-2434 -0.268 94.0 -0.175 89.0 noncon
hsa-miR-502-3p 94-100 -0.267 95.0 -0.257 96.0 noncon
hsa-miR-501-3p 94-100 -0.267 95.0 -0.257 96.0 noncon
hsa-miR-6069 305-311 -0.264 92.0 -0.249 94.0 noncon
hsa-miR-6841-5p 2140-2146 -0.263 91.0 -0.248 93.0 noncon
hsa-miR-1913 1984-1991 -0.262 94.0 -0.247 95.0 noncon
hsa-miR-3155b 1076-1083 -0.262 97.0 -0.247 97.0 noncon
hsa-miR-3155a 1076-1083 -0.262 97.0 -0.247 97.0 noncon
hsa-miR-4519 1009-1015 -0.261 95.0 -0.246 96.0 noncon
hsa-miR-376a-3p 2157-2164 -0.26 93.0 -0.245 95.0 con
hsa-miR-376b-3p 2157-2164 -0.26 93.0 -0.245 95.0 con
hsa-miR-4489 300-306 -0.258 94.0 -0.243 95.0 noncon
hsa-miR-6788-5p 1001-1008 -0.256 97.0 -0.242 97.0 noncon
hsa-miR-103a-2-5p 1323-1330 -0.255 95.0 -0.241 96.0 noncon
hsa-miR-1-3p 114-120 -0.254 94.0 -0.244 95.0 con
hsa-miR-206 114-120 -0.254 94.0 -0.244 95.0 con
hsa-miR-605-3p 1785-1792 -0.253 92.0 -0.239 93.0 noncon