GlyClass

GlycoEnzDB
Comprehensive glycoEnzyme database and dynamic pathway generation

Description: The GlycoEnzDB is a manually curated glycoEnzyme database, primarily focused on humans. It covers 390 enzymes across 28 pathway maps. Facilities are also available to create custom glycosylation reaction pathways using experimental data in SBML format and for pathway simulation
Contacts: Sriram Neelamegham (neel@buffalo.edu), Yusen Zhou (yusenzho@buffalo.edu) or Ted Groth (tgroth@buffalo.edu).

GlycoEnzDB
Create glycosylation pathway
Custom database generation
Instructions/Reference
 
Pathway Map
PIGA



General Information
Gene Symbol:   
PIGA
Protein name:   
Phosphatidylinositol N-acetylglucosaminyltransferase subunit A
Enzyme Class:   
Glycosyltransferase
CAZy Family:   
GT4
E.C. No:   
2.4.1.198
Organs:   
Reaction (SNFG):   
+ PI →
+ UDP
Reaction (IUBMB):   
No specified reaction.
About

• GPI-GnT: GlcNAcT activity that is necessary to form the core glycan structure on GPI-anchored proteins
• Subunit PIGA has catalytic activity, while PIGC, PIGH, PIGQ, PIGP and PIGY have other essential functions
• DPM2 is regulatory and it functions to enhance GPI-GnT activity

Related Pathway
KEGG ORTHOLOGY:   
K03857
KEGG Pathway:   
ko00563  ko01100 
Reactome:   
R-HSA-162710
Related Database
Organism:   
Homo sapiens
Uniprot:   
P37287 
NCBI GeneID:   
5277
GlyMap GeneID:   
5277
DNA aRefSeq:   
NM_002641.3 
Protein RefSeq:   
NP_002632.1 
BRENDA:   
Kinetic info.
OMIM number:   
311770
GeneCards database:   
PIGA
Enzyme Specificity
Glycan Type:  
     GPI
Compartment:  
     ER
Substrate:  
     PI
Product:  
     GlcNAc(a1-?)PI
Simulation Parameters
Mechaelis-Menten parameters (suggested)
VM:       (pmol/cell • hr)       KM:       (pM)
Relative conc of enzymes in compartment.(sum will be normalized to 1)
ER Cis- Medial- Trans- TGN










Expression Data in Tissue {ref. 5} Source from NCBI gene database
adrenal appendix bone marrow brain colon duodenum endometrium esophagus fat gall bladder heart kidney liver lung lymph node ovary pancreas placenta prostate salivary gland skin small intestine spleen stomach testis thyroid urinary bladder 12 10 8 6 4 2 0 RPKM
Expression Data in Cells {ref. 13} Source from Cancer Cell Line Encyclopedia database
Kidney Lung Breast Prostate Large intestine Ovary HL60 haematopoietic and lymphoid tissue Skin Central nervous system 786O A498 ACHN CAKI1 UO31 A549 EKVX HOP62 HOP92 NCIH226 NCIH23 NCIH322 NCIH460 NCIH522 SHP77 BT549 HS578T MCF7 MDAMB231 MDAMB468 T47D DU145 PC3 HCT116 HCT15 HT29 KM12 SW620 OVCAR4 OVCAR8 IGROV1 SKOV3 HL60 K562 RPMI8226 LOXIMVI MALME3M RPMI7951 SKMEL28 SKMEL5 UACC257 UACC62 SF268 SF295 SF539 SNB75 20 10 0 Transcripts per million (TPM)
Transcription factor {ref. 10}
No high degree of confidence data
miRNA-GlycoEnzyme Interactome {ref. 9}
miRNA Name Pos. Context Score Frac. Wt. Context Frac. Conserved Quality
hsa-miR-4746-5p 841-848 -0.681 98.0 -0.669 98.0 noncon
hsa-miR-598-5p 734-741 -0.559 99.0 -0.549 99.0 noncon
hsa-miR-3663-5p 2028-2035 -0.499 99.0 -0.49 99.0 noncon
hsa-miR-5689 161-168 -0.49 97.0 -0.482 98.0 noncon
hsa-miR-6755-3p 1317-1324 -0.482 98.0 -0.474 98.0 noncon
hsa-miR-6510-5p 1890-1897 -0.474 99.0 -0.466 99.0 noncon
hsa-miR-8073 721-728 -0.469 99.0 -0.461 99.0 noncon
hsa-miR-221-5p 721-728 -0.469 99.0 -0.461 99.0 noncon
hsa-miR-4520-2-3p 2015-2022 -0.459 98.0 -0.451 98.0 noncon
hsa-miR-3170 1164-1171 -0.454 98.0 -0.446 99.0 noncon
hsa-miR-328-3p 312-319 -0.439 98.0 -0.432 98.0 con
hsa-miR-6845-3p 209-216 -0.438 99.0 -0.431 99.0 noncon
hsa-miR-4500 116-122 -0.429 96.0 -0.422 97.0 con
hsa-miR-6855-5p 1164-1171 -0.425 98.0 -0.418 99.0 noncon
hsa-let-7a-5p 116-122 -0.418 96.0 -0.411 97.0 con
hsa-let-7i-5p 116-122 -0.418 96.0 -0.411 97.0 con
hsa-let-7g-5p 116-122 -0.418 96.0 -0.411 97.0 con
hsa-let-7c-5p 116-122 -0.418 96.0 -0.411 97.0 con
hsa-let-7f-5p 116-122 -0.418 96.0 -0.411 97.0 con
hsa-let-7b-5p 116-122 -0.418 96.0 -0.411 97.0 con
hsa-miR-98-5p 116-122 -0.418 96.0 -0.411 97.0 con
hsa-let-7e-5p 116-122 -0.418 96.0 -0.411 97.0 con
hsa-miR-1257 1323-1330 -0.417 99.0 -0.41 99.0 noncon
hsa-miR-133a-5p 547-554 -0.415 98.0 -0.408 98.0 noncon
hsa-miR-519a-3p 191-198 -0.413 99.0 -0.406 99.0 noncon
hsa-miR-519b-3p 191-198 -0.413 99.0 -0.406 99.0 noncon
hsa-miR-519c-3p 191-198 -0.413 99.0 -0.406 99.0 noncon
hsa-let-7d-5p 116-122 -0.408 95.0 -0.401 96.0 con
hsa-miR-4458 116-122 -0.397 95.0 -0.39 96.0 con
hsa-miR-329-3p 1183-1190 -0.393 99.0 -0.387 99.0 noncon
hsa-miR-148a-3p 1373-1380 -0.39 97.0 -0.384 98.0 noncon
hsa-miR-148b-3p 1373-1380 -0.39 97.0 -0.384 98.0 noncon
hsa-miR-152-3p 1373-1380 -0.39 97.0 -0.384 98.0 noncon
hsa-miR-362-3p 1183-1190 -0.385 99.0 -0.379 99.0 noncon
hsa-miR-4790-3p 638-645 -0.381 99.0 -0.375 99.0 noncon
hsa-miR-7151-3p 1734-1741 -0.377 98.0 -0.371 98.0 noncon
hsa-miR-7703 1430-1437 -0.374 99.0 -0.368 99.0 noncon
hsa-miR-202-5p 1565-1572 -0.371 98.0 -0.365 99.0 noncon
hsa-miR-5095 1734-1741 -0.364 98.0 -0.358 98.0 noncon
hsa-miR-376b-5p 1174-1181 -0.363 97.0 -0.357 97.0 noncon
hsa-miR-618 93-100 -0.359 99.0 -0.353 99.0 noncon
hsa-miR-7161-3p 1711-1718 -0.347 98.0 -0.341 99.0 noncon
hsa-miR-455-3p.1 1478-1485 -0.344 98.0 -0.338 98.0 noncon
hsa-miR-4694-5p 355-362 -0.343 97.0 -0.337 98.0 noncon
hsa-miR-7847-3p 875-882 -0.342 96.0 -0.336 97.0 noncon
hsa-miR-7850-5p 2016-2023 -0.341 99.0 -0.336 99.0 noncon
hsa-miR-2117 212-219 -0.339 97.0 -0.333 97.0 noncon
hsa-miR-3926 1590-1597 -0.338 98.0 -0.333 98.0 noncon
hsa-miR-6851-3p 311-318 -0.338 97.0 -0.333 97.0 noncon
hsa-miR-365b-3p 329-335 -0.337 97.0 -0.331 98.0 noncon
hsa-miR-365a-3p 329-335 -0.337 97.0 -0.331 98.0 noncon
hsa-miR-5572 1165-1172 -0.331 98.0 -0.326 98.0 noncon
hsa-miR-548v 263-270 -0.331 96.0 -0.326 97.0 noncon
hsa-miR-200a-5p 16-22 -0.328 98.0 -0.328 99.0 noncon
hsa-miR-200b-5p 16-22 -0.328 98.0 -0.328 99.0 noncon
hsa-miR-6728-3p 267-274 -0.324 98.0 -0.319 98.0 noncon
hsa-miR-5009-3p 205-212 -0.322 99.0 -0.317 99.0 noncon
hsa-miR-562 1894-1901 -0.319 98.0 -0.314 98.0 noncon
hsa-miR-199b-3p 250-257 -0.316 96.0 -0.311 97.0 noncon
hsa-miR-199a-3p 250-257 -0.316 96.0 -0.311 97.0 noncon
hsa-miR-193a-3p 340-346 -0.315 94.0 -0.31 96.0 noncon
hsa-miR-193b-3p 340-346 -0.315 94.0 -0.31 96.0 noncon
hsa-miR-6871-3p 303-309 -0.315 97.0 -0.31 98.0 noncon
hsa-miR-550b-3p 527-534 -0.313 97.0 -0.308 98.0 noncon
hsa-miR-3660 1847-1853 -0.313 97.0 -0.308 98.0 noncon
hsa-miR-4695-3p 678-685 -0.312 97.0 -0.307 97.0 noncon
hsa-miR-4719 1935-1942 -0.307 99.0 -0.302 99.0 noncon
hsa-miR-3179 997-1004 -0.304 95.0 -0.299 96.0 noncon
hsa-miR-1909-3p 564-570 -0.303 95.0 -0.298 95.0 noncon
hsa-miR-582-5p 1580-1587 -0.3 98.0 -0.295 98.0 noncon
hsa-miR-4698 2040-2047 -0.3 99.0 -0.295 99.0 noncon
hsa-miR-7641 679-686 -0.299 97.0 -0.294 98.0 noncon
hsa-miR-5195-5p 1456-1462 -0.295 96.0 -0.29 97.0 noncon
hsa-miR-4295 190-196 -0.295 96.0 -0.29 97.0 con
hsa-miR-4777-5p 1446-1453 -0.291 97.0 -0.286 98.0 noncon
hsa-miR-3187-5p 1142-1149 -0.287 97.0 -0.282 98.0 noncon
hsa-miR-4503 1651-1657 -0.286 95.0 -0.282 97.0 noncon
hsa-miR-6860 1142-1149 -0.284 97.0 -0.28 98.0 noncon
hsa-miR-6722-3p 564-570 -0.284 93.0 -0.28 94.0 noncon
hsa-miR-4793-3p 862-869 -0.283 97.0 -0.278 98.0 noncon
hsa-miR-376c-5p 1174-1181 -0.282 96.0 -0.278 97.0 noncon
hsa-miR-183-5p.2 1553-1559 -0.28 93.0 -0.276 95.0 con
hsa-miR-3914 519-525 -0.276 96.0 -0.272 97.0 noncon
hsa-miR-454-3p 190-196 -0.276 95.0 -0.272 97.0 con
hsa-miR-4666a-5p 505-512 -0.275 96.0 -0.271 97.0 noncon
hsa-miR-1285-3p 1142-1149 -0.274 97.0 -0.27 97.0 noncon
hsa-miR-3923 747-754 -0.274 94.0 -0.27 96.0 noncon
hsa-miR-5189-5p 1142-1149 -0.274 97.0 -0.27 98.0 noncon
hsa-miR-8064 1038-1045 -0.272 96.0 -0.268 97.0 noncon
hsa-miR-5094 1375-1382 -0.27 96.0 -0.266 97.0 noncon
hsa-miR-4799-3p 710-716 -0.269 96.0 -0.265 97.0 noncon
hsa-miR-3065-3p 304-311 -0.267 96.0 -0.263 97.0 noncon
hsa-miR-222-5p 251-258 -0.265 95.0 -0.261 97.0 noncon
hsa-miR-30e-5p 379-386 -0.264 93.0 -0.26 94.0 con
hsa-miR-301b-3p 190-196 -0.262 94.0 -0.258 96.0 con
hsa-miR-301a-3p 190-196 -0.262 94.0 -0.258 96.0 con
hsa-miR-3666 190-196 -0.262 95.0 -0.258 96.0 con
hsa-miR-130b-3p 190-196 -0.262 94.0 -0.258 96.0 con
hsa-miR-587 53-60 -0.262 98.0 -0.262 98.0 noncon
hsa-miR-519e-3p 1252-1258 -0.26 93.0 -0.256 95.0 noncon
hsa-miR-4328 1988-1995 -0.259 98.0 -0.255 98.0 noncon
hsa-miR-4732-5p 1884-1890 -0.256 95.0 -0.252 96.0 noncon
hsa-miR-548s 1591-1597 -0.256 97.0 -0.252 97.0 noncon
hsa-miR-510-5p 172-178 -0.256 95.0 -0.252 96.0 noncon
hsa-miR-325 746-752 -0.254 93.0 -0.25 95.0 noncon
hsa-miR-3926 337-344 -0.253 95.0 -0.249 96.0 noncon
hsa-miR-130a-3p 190-196 -0.251 94.0 -0.247 95.0 con
hsa-miR-219b-3p 166-172 -0.25 95.0 -0.246 96.0 noncon