GlyClass

GlycoEnzDB
Comprehensive glycoEnzyme database and dynamic pathway generation

Description: The GlycoEnzDB is a manually curated glycoEnzyme database, primarily focused on humans. It covers 390 enzymes across 28 pathway maps. Facilities are also available to create custom glycosylation reaction pathways using experimental data in SBML format and for pathway simulation
Contacts: Sriram Neelamegham (neel@buffalo.edu), Yusen Zhou (yusenzho@buffalo.edu) or Ted Groth (tgroth@buffalo.edu).

GlycoEnzDB
Create glycosylation pathway
Custom database generation
Instructions/Reference
 
Pathway Map
B3GALNT1



General Information
Gene Symbol:   
B3GALNT1
Protein name:   
UDP-GalNAc:beta-1,3-N-acetylgalactosaminyltransferase 1
Enzyme Class:   
Glycosyltransferase
CAZy Family:   
GT31
E.C. No:   
2.4.1.79
Organs:   
Reaction (SNFG):   
+
→
+ UDP
Reaction (IUBMB):   
No specified reaction.
About

• This is globoside synthase as it generates the P antigen: globotetraosylceramide, Gb4, GalNAcβ1-3Galα1-4Galβ1-4G1c-Cer.
• B3galnt1 gene disruption in mice results in embryonic lethality, early, probably prior to implantation. A4GalT mice lacking the Globo series are born normally. Thus, B3galnt1 may have additional unidentified roles.

Related Pathway
KEGG ORTHOLOGY:   
K00719
KEGG Pathway:   
ko00601  ko00603  ko01100 
Reactome:   
R-HSA-1660662
Related Database
Organism:   
Homo sapiens
Uniprot:   
O75752 
NCBI GeneID:   
8706
GlyMap GeneID:   
8706
DNA aRefSeq:   
NM_001038628.1 
Protein RefSeq:   
NP_001033717.1 
BRENDA:   
Kinetic info.
OMIM number:   
603094
GeneCards database:   
B3GALNT1
Enzyme Specificity
Glycan Type:  
     GSL
Compartment:  
     Cis, Medial, Trans, TGN
Substrate:  
     Gal(a1-4)Gal(b1-4)Glc(b1-?)Cer
Product:  
     GalNAc(b1-3)Gal(a1-4)Gal(b1-4)Glc(b1-?)Cer
Simulation Parameters
Mechaelis-Menten parameters (suggested)
VM:    5.75e-04    (pmol/cell • hr)       KM:    1.50e+08    (pM)
Relative conc of enzymes in compartment.(sum will be normalized to 1)
ER Cis- Medial- Trans- TGN










Expression Data in Tissue {ref. 5} Source from NCBI gene database
adrenal appendix bone marrow brain colon duodenum endometrium esophagus fat gall bladder heart kidney liver lung lymph node ovary pancreas placenta prostate salivary gland skin small intestine spleen stomach testis thyroid urinary bladder 20 10 0 RPKM
Expression Data in Cells {ref. 13} Source from Cancer Cell Line Encyclopedia database
Kidney Lung Breast Prostate Large intestine Ovary HL60 haematopoietic and lymphoid tissue Skin Central nervous system 786O A498 ACHN CAKI1 UO31 A549 EKVX HOP62 HOP92 NCIH226 NCIH23 NCIH322 NCIH460 NCIH522 SHP77 BT549 HS578T MCF7 MDAMB231 MDAMB468 T47D DU145 PC3 HCT116 HCT15 HT29 KM12 SW620 OVCAR4 OVCAR8 IGROV1 SKOV3 HL60 K562 RPMI8226 LOXIMVI MALME3M RPMI7951 SKMEL28 SKMEL5 UACC257 UACC62 SF268 SF295 SF539 SNB75 80 60 40 20 0 Transcripts per million (TPM)
Transcription factor {ref. 10}
No high degree of confidence data
miRNA-GlycoEnzyme Interactome {ref. 9}
miRNA Name Pos. Context Score Frac. Wt. Context Frac. Conserved Quality
hsa-miR-645 18-25 -0.656 99.0 -0.656 99.0 noncon
hsa-miR-6748-3p 27-34 -0.63 99.0 -0.63 99.0 noncon
hsa-miR-133b 244-251 -0.539 98.0 -0.527 99.0 con
hsa-miR-133a-3p.2 244-251 -0.539 98.0 -0.527 99.0 con
hsa-miR-4718 1795-1802 -0.526 99.0 -0.041 61.0 noncon
hsa-miR-140-3p.2 736-743 -0.52 99.0 -0.359 99.0 con
hsa-miR-548ao-5p 541-548 -0.503 99.0 -0.348 98.0 noncon
hsa-miR-548ax 541-548 -0.503 99.0 -0.348 98.0 noncon
hsa-miR-6792-3p 193-200 -0.5 99.0 -0.489 99.0 noncon
hsa-miR-3158-3p 1089-1096 -0.495 99.0 -0.074 77.0 noncon
hsa-miR-3157-3p 1261-1268 -0.484 99.0 -0.072 77.0 noncon
hsa-miR-34c-5p 1776-1783 -0.482 98.0 -0.038 50.0 noncon
hsa-miR-449b-5p 1776-1783 -0.482 98.0 -0.038 50.0 noncon
hsa-miR-5585-5p 496-503 -0.477 99.0 -0.466 99.0 noncon
hsa-miR-449a 1776-1783 -0.464 98.0 -0.037 51.0 noncon
hsa-miR-4666b 614-621 -0.456 99.0 -0.317 97.0 noncon
hsa-miR-34a-5p 1776-1783 -0.456 97.0 -0.037 49.0 noncon
hsa-miR-514b-3p 429-436 -0.439 99.0 -0.429 99.0 noncon
hsa-miR-514a-3p 429-436 -0.439 99.0 -0.429 99.0 noncon
hsa-miR-4691-5p 193-200 -0.427 99.0 -0.418 99.0 noncon
hsa-miR-4641 934-941 -0.412 97.0 -0.063 63.0 noncon
hsa-miR-378a-5p 1317-1324 -0.407 99.0 -0.062 85.0 noncon
hsa-miR-643 581-588 -0.401 98.0 -0.28 97.0 noncon
hsa-miR-942-5p 748-755 -0.387 98.0 -0.271 97.0 noncon
hsa-miR-337-5p 1704-1710 -0.381 96.0 -0.031 32.0 noncon
hsa-miR-4635 499-506 -0.381 99.0 -0.373 99.0 noncon
hsa-miR-501-5p 592-599 -0.374 98.0 -0.262 97.0 noncon
hsa-miR-425-5p 958-965 -0.366 99.0 -0.056 83.0 noncon
hsa-miR-6505-3p 818-825 -0.364 97.0 -0.075 75.0 noncon
hsa-miR-27b-5p 309-316 -0.361 98.0 -0.353 98.0 noncon
hsa-miR-3925-3p 134-141 -0.353 96.0 -0.345 97.0 noncon
hsa-miR-6794-3p 991-998 -0.35 97.0 -0.054 75.0 noncon
hsa-miR-4276 445-452 -0.348 98.0 -0.34 98.0 noncon
hsa-miR-3692-3p 68-75 -0.343 98.0 -0.343 98.0 noncon
hsa-miR-2909 1091-1097 -0.341 97.0 -0.053 78.0 noncon
hsa-miR-3610 1112-1118 -0.328 93.0 -0.051 39.0 noncon
hsa-miR-4445-5p 1042-1049 -0.325 96.0 -0.051 67.0 noncon
hsa-miR-3171 659-666 -0.324 96.0 -0.228 93.0 noncon
hsa-miR-6759-3p 1301-1308 -0.323 96.0 -0.05 71.0 noncon
hsa-miR-496.1 1358-1365 -0.32 96.0 -0.05 65.0 noncon
hsa-miR-802 297-304 -0.316 98.0 -0.309 98.0 noncon
hsa-miR-6837-3p 993-1000 -0.314 97.0 -0.049 71.0 noncon
hsa-miR-1233-3p 1450-1457 -0.313 97.0 -0.047 64.0 noncon
hsa-miR-5088-3p 918-925 -0.309 97.0 -0.048 73.0 noncon
hsa-miR-125b-2-3p 154-161 -0.308 98.0 -0.301 98.0 noncon
hsa-miR-16-1-3p 1825-1832 -0.307 97.0 -0.026 67.0 noncon
hsa-miR-4772-3p 508-514 -0.306 98.0 -0.299 99.0 noncon
hsa-miR-3692-3p 40-47 -0.306 97.0 -0.306 98.0 noncon
hsa-miR-6828-3p 1673-1680 -0.306 97.0 -0.026 65.0 noncon
hsa-miR-5001-3p 909-916 -0.299 96.0 -0.047 70.0 noncon
hsa-miR-1199-5p 1452-1459 -0.299 96.0 -0.045 74.0 noncon
hsa-miR-6751-3p 1452-1459 -0.296 96.0 -0.045 72.0 noncon
hsa-miR-3618 271-277 -0.295 90.0 -0.289 93.0 noncon
hsa-miR-215-5p 686-692 -0.289 97.0 -0.204 95.0 noncon
hsa-miR-3185 440-447 -0.289 97.0 -0.283 97.0 noncon
hsa-miR-192-5p 686-692 -0.285 97.0 -0.201 95.0 noncon
hsa-miR-6864-5p 175-182 -0.284 96.0 -0.278 97.0 noncon
hsa-miR-5701 1837-1844 -0.283 97.0 -0.024 73.0 noncon
hsa-miR-4464 1765-1772 -0.283 98.0 -0.024 64.0 noncon
hsa-miR-6738-3p 910-917 -0.281 95.0 -0.044 72.0 noncon
hsa-miR-548b-3p 1271-1277 -0.273 97.0 -0.043 83.0 noncon
hsa-miR-335-5p 1350-1356 -0.272 97.0 -0.043 82.0 noncon
hsa-miR-891b 1004-1011 -0.269 94.0 -0.043 64.0 noncon
hsa-miR-4660 1054-1060 -0.265 94.0 -0.042 66.0 noncon
hsa-miR-3649 1653-1659 -0.262 91.0 -0.022 40.0 noncon
hsa-miR-192-5p 1193-1200 -0.26 96.0 -0.041 67.0 noncon
hsa-miR-6514-5p 666-672 -0.26 96.0 -0.184 94.0 noncon
hsa-miR-3064-3p 1580-1586 -0.26 92.0 -0.022 50.0 noncon
hsa-miR-215-5p 1193-1200 -0.257 96.0 -0.041 64.0 noncon
hsa-miR-217 1235-1241 -0.256 97.0 -0.041 79.0 noncon
hsa-miR-4685-3p 920-927 -0.253 96.0 -0.04 75.0 noncon
hsa-miR-4436b-3p 1020-1026 -0.253 93.0 -0.04 62.0 noncon
hsa-miR-6879-5p 1020-1026 -0.253 93.0 -0.04 62.0 noncon
hsa-miR-548ax 497-503 -0.252 95.0 -0.247 96.0 noncon
hsa-miR-6507-3p 716-722 -0.25 96.0 -0.177 95.0 noncon