GlyClass

GlycoEnzDB
Comprehensive glycoEnzyme database and dynamic pathway generation

Description: The GlycoEnzDB is a manually curated glycoEnzyme database, primarily focused on humans. It covers 390 enzymes across 28 pathway maps. Facilities are also available to create custom glycosylation reaction pathways using experimental data in SBML format and for pathway simulation
Contacts: Sriram Neelamegham (neel@buffalo.edu), Yusen Zhou (yusenzho@buffalo.edu) or Ted Groth (tgroth@buffalo.edu).

GlycoEnzDB
Create glycosylation pathway
Custom database generation
Instructions/Reference
 
Pathway Map
GAL3ST1



General Information
Gene Symbol:   
GAL3ST1
Protein name:   
Galactosylceramide sulfotransferase
Enzyme Class:   
Sulfate synthase
CAZy Family:   
E.C. No:   
2.8.2.11
Organs:   
Reaction (SNFG):   
PAPS +
→
+ PAP
PAPS +
→
+ PAP
Reaction (IUBMB):   
No specified reaction.
About

• There are 4 enzymes that perform 3-O-sulfation of Gal.
• Gal3ST1: Acts on GalCer and LacCer to create sulfated glycolipids. Catalyzes the synthesis of sulfatides and seminolpids.
• Gal3ST2: Galβ1,4GlcNAc of O- and N-glycan, can act on sulfated substrates to form bisulfated carbohydrates like 3′,6-disulfo LewisX and 3′,6'-disulfo LewisX. This can also form 3'-sulfo-Le a and 3-O-sulfo-LeX determinants and it can act on lipid substrates
• Gal3ST3 Galβ1,4GlcNAc of O- and N-glycan and Galβ1,4GalNAc of GloboH structure. Can act on sulfated substrates to form bisulfated carbohydrates
• Gal3ST4 Galβ1,4GalNAc of Globo glycolipids and glycopeptides

Related Pathway
KEGG ORTHOLOGY:   
K01019
KEGG Pathway:   
ko00565  ko00600  ko01100 
Reactome:   
No Record
Related Database
Organism:   
Homo sapiens
Uniprot:   
Q99999 
NCBI GeneID:   
9514
GlyMap GeneID:   
9514
DNA aRefSeq:   
NM_001318103.1 
Protein RefSeq:   
NP_001305032.1 
BRENDA:   
Kinetic info.
OMIM number:   
602300
GeneCards database:   
GAL3ST1
Enzyme Specificity
Glycan Type:  
     GSL
Compartment:  
     Cis, Medial, Trans, TGN
Substrate:  
     Gal(b1-?)Cer or Gal(b1-4)Glc(b1-?)Cer
Product:  
     Gal(b1-? [3S])Cer or Gal(b1-4 [3S])Glc(b1-?)Cer
Simulation Parameters
Mechaelis-Menten parameters (suggested)
VM:       (pmol/cell • hr)       KM:       (pM)
Relative conc of enzymes in compartment.(sum will be normalized to 1)
ER Cis- Medial- Trans- TGN










Expression Data in Tissue {ref. 5} Source from NCBI gene database
adrenal appendix bone marrow brain colon duodenum endometrium esophagus fat gall bladder heart kidney liver lung lymph node ovary pancreas placenta prostate salivary gland skin small intestine spleen stomach testis thyroid urinary bladder 4 2 0 RPKM
Expression Data in Cells {ref. 13} Source from Cancer Cell Line Encyclopedia database
Kidney Lung Breast Prostate Large intestine Ovary HL60 haematopoietic and lymphoid tissue Skin Central nervous system 786O A498 ACHN CAKI1 UO31 A549 EKVX HOP62 HOP92 NCIH226 NCIH23 NCIH322 NCIH460 NCIH522 SHP77 BT549 HS578T MCF7 MDAMB231 MDAMB468 T47D DU145 PC3 HCT116 HCT15 HT29 KM12 SW620 OVCAR4 OVCAR8 IGROV1 SKOV3 HL60 K562 RPMI8226 LOXIMVI MALME3M RPMI7951 SKMEL28 SKMEL5 UACC257 UACC62 SF268 SF295 SF539 SNB75 160 140 120 100 80 60 40 20 0 Transcripts per million (TPM)
Transcription factor {ref. 10}
No high degree of confidence data
miRNA-GlycoEnzyme Interactome {ref. 9}
miRNA Name Pos. Context Score Frac. Wt. Context Frac. Conserved Quality
hsa-miR-6889-5p 233-240 -0.85 99.0 -0.85 99.0 noncon
hsa-miR-6777-5p 233-240 -0.828 99.0 -0.828 99.0 noncon
hsa-miR-4697-5p 76-83 -0.416 97.0 -0.416 98.0 noncon
hsa-miR-1237-5p 76-83 -0.411 97.0 -0.411 98.0 noncon
hsa-miR-4774-5p 206-212 -0.411 98.0 -0.411 98.0 noncon
hsa-miR-149-3p 231-237 -0.405 98.0 -0.405 98.0 noncon
hsa-miR-4728-5p 231-237 -0.393 98.0 -0.393 98.0 noncon
hsa-miR-4488 76-83 -0.392 97.0 -0.392 97.0 noncon
hsa-miR-3936 223-229 -0.389 98.0 -0.389 99.0 noncon
hsa-miR-6883-5p 231-237 -0.383 98.0 -0.383 98.0 noncon
hsa-miR-593-5p 93-100 -0.378 96.0 -0.378 97.0 noncon
hsa-miR-6785-5p 231-237 -0.371 98.0 -0.371 98.0 noncon
hsa-miR-8088 182-188 -0.37 94.0 -0.37 96.0 noncon
hsa-miR-582-3p 208-214 -0.369 98.0 -0.369 99.0 noncon
hsa-miR-6825-5p 232-238 -0.368 94.0 -0.368 95.0 noncon
hsa-miR-5003-5p 302-308 -0.366 98.0 -0.004 26.0 noncon
hsa-miR-6842-3p 144-151 -0.361 98.0 -0.361 98.0 noncon
hsa-miR-6825-5p 261-267 -0.361 94.0 -0.361 95.0 noncon
hsa-miR-4747-3p 175-181 -0.351 98.0 -0.351 99.0 noncon
hsa-miR-6797-5p 230-236 -0.351 97.0 -0.351 98.0 noncon
hsa-miR-193b-5p 221-227 -0.35 94.0 -0.35 96.0 noncon
hsa-miR-6773-5p 156-162 -0.345 96.0 -0.345 97.0 noncon
hsa-miR-542-5p 234-240 -0.343 95.0 -0.343 96.0 noncon
hsa-miR-6724-5p 156-162 -0.342 96.0 -0.342 97.0 noncon
hsa-miR-200b-3p 190-196 -0.336 99.0 -0.336 99.0 con
hsa-miR-429 190-196 -0.336 99.0 -0.336 99.0 con
hsa-miR-200c-3p 190-196 -0.336 99.0 -0.336 99.0 con
hsa-miR-1249-5p 230-236 -0.329 97.0 -0.329 97.0 noncon
hsa-miR-6865-5p 97-103 -0.324 97.0 -0.324 97.0 noncon
hsa-miR-3682-5p 284-291 -0.32 97.0 -0.097 84.0 noncon
hsa-miR-1295a 176-182 -0.319 96.0 -0.319 98.0 noncon
hsa-miR-3612 259-265 -0.317 97.0 -0.317 98.0 noncon
hsa-miR-1827 258-264 -0.316 96.0 -0.316 97.0 noncon
hsa-miR-124-5p 163-170 -0.304 94.0 -0.304 96.0 noncon
hsa-miR-6815-5p 97-103 -0.302 96.0 -0.302 97.0 noncon
hsa-miR-5681a 225-231 -0.3 97.0 -0.3 98.0 noncon
hsa-miR-6134 98-105 -0.297 94.0 -0.297 96.0 noncon
hsa-miR-3936 264-270 -0.285 96.0 -0.285 97.0 noncon
hsa-miR-629-5p 220-226 -0.282 97.0 -0.282 98.0 noncon
hsa-miR-650 259-265 -0.273 96.0 -0.273 97.0 noncon
hsa-miR-4532 108-114 -0.267 90.0 -0.267 92.0 noncon
hsa-miR-1205 38-45 -0.264 97.0 -0.264 98.0 noncon
hsa-miR-6739-5p 227-233 -0.256 96.0 -0.256 97.0 noncon
hsa-miR-7641 185-191 -0.255 96.0 -0.255 97.0 noncon