GlyClass

GlycoEnzDB
Comprehensive glycoEnzyme database and dynamic pathway generation

Description: The GlycoEnzDB is a manually curated glycoEnzyme database, primarily focused on humans. It covers 390 enzymes across 28 pathway maps. Facilities are also available to create custom glycosylation reaction pathways using experimental data in SBML format and for pathway simulation
Contacts: Sriram Neelamegham (neel@buffalo.edu), Yusen Zhou (yusenzho@buffalo.edu) or Ted Groth (tgroth@buffalo.edu).

GlycoEnzDB
Create glycosylation pathway
Custom database generation
Instructions/Reference
 
Pathway Map
HEXA



General Information
Gene Symbol:   
HEXA
Protein name:   
Beta-hexosaminidase subunit alpha
Enzyme Class:   
Glycosidase
CAZy Family:   
GH20
E.C. No:   
3.2.1.52
Tissues:   
Muscular, Epithelial, Connective, Nervous
Organs:   
Reaction (SNFG):   
→
Reaction (IUBMB):   
No specified reaction.
About

• Beta hexosaminidase A and B interact with one another to degrade the GM2 ganglioside in lysosomes by removing GalNAc. These enzymes are important for regulating glycolipid homeostasis in the central nervous system.
• Defects in HEXA result in Tay Sachs disease, a condition which results in the buildup of lipids in the brain. This results in seizures, paralysis, and eventually death at an early age.
• HAEXA/B also facilitate glycoproteins (N- and O-) and keratin sulfate degradation

Related Pathway
KEGG ORTHOLOGY:   
KEGG Pathway:   
No Record 
Reactome:   
R-HSA-1660662
Related Database
Organism:   
Homo sapiens
Uniprot:   
P06865 
NCBI GeneID:   
3073
GlyMap GeneID:   
3073
DNA aRefSeq:   
NM_000520.5 
Protein RefSeq:   
NP_000511.2 
BRENDA:   
Kinetic info.
OMIM number:   
606869
GeneCards database:   
HEXA
Enzyme Specificity
Glycan Type:  
     GSL
Compartment:  
     
Substrate:  
     GalNAc(b1-?)
Product:  
     
Constraints:  
     None
Simulation Parameters
Mechaelis-Menten parameters (suggested)
VM:       (pmol/cell • hr)       KM:       (pM)
Relative conc of enzymes in compartment.(sum will be normalized to 1)
ER Cis- Medial- Trans- TGN










Expression Data in Tissue {ref. 5} Source from NCBI gene database
adrenal appendix bone marrow brain colon duodenum endometrium esophagus fat gall bladder heart kidney liver lung lymph node ovary pancreas placenta prostate salivary gland skin small intestine spleen stomach testis thyroid urinary bladder 50 40 30 20 10 0 RPKM
Expression Data in Cells {ref. 13} Source from Cancer Cell Line Encyclopedia database
Kidney Lung Breast Prostate Large intestine Ovary HL60 haematopoietic and lymphoid tissue Skin Central nervous system 786O A498 ACHN CAKI1 UO31 A549 EKVX HOP62 HOP92 NCIH226 NCIH23 NCIH322 NCIH460 NCIH522 SHP77 BT549 HS578T MCF7 MDAMB231 MDAMB468 T47D DU145 PC3 HCT116 HCT15 HT29 KM12 SW620 OVCAR4 OVCAR8 IGROV1 SKOV3 HL60 K562 RPMI8226 LOXIMVI MALME3M RPMI7951 SKMEL28 SKMEL5 UACC257 UACC62 SF268 SF295 SF539 SNB75 300 200 100 0 Transcripts per million (TPM)
Transcription factor {ref. 10}
No high degree of confidence data
miRNA-GlycoEnzyme Interactome {ref. 9}
miRNA Name Pos. Context Score Frac. Wt. Context Frac. Conserved Quality
hsa-miR-4665-3p 1133-1140 -0.487 99.0 -0.057 54.0 noncon
hsa-miR-122-3p 227-233 -0.462 98.0 -0.073 63.0 noncon
hsa-miR-146b-3p 3019-3026 -0.428 99.0 -0.051 74.0 noncon
hsa-miR-5698 524-531 -0.392 94.0 -0.063 50.0 noncon
hsa-miR-7111-5p 524-531 -0.392 94.0 -0.063 50.0 noncon
hsa-miR-339-3p 146-152 -0.392 90.0 -0.392 93.0 noncon
hsa-miR-4460 2198-2205 -0.388 96.0 -0.046 57.0 noncon
hsa-miR-572 2443-2450 -0.385 95.0 -0.046 49.0 noncon
hsa-miR-6870-5p 524-531 -0.384 93.0 -0.062 51.0 noncon
hsa-miR-4723-5p 524-531 -0.376 93.0 -0.061 49.0 noncon
hsa-miR-548al 2758-2765 -0.373 95.0 -0.045 34.0 noncon
hsa-miR-4716-5p 191-198 -0.348 98.0 -0.102 90.0 noncon
hsa-miR-4445-3p 2758-2765 -0.344 94.0 -0.042 34.0 noncon
hsa-miR-6871-3p 3084-3090 -0.33 97.0 -0.04 68.0 noncon
hsa-miR-216b-3p 219-226 -0.324 98.0 -0.053 80.0 noncon
hsa-miR-3649 1907-1914 -0.321 95.0 -0.039 50.0 noncon
hsa-miR-6501-5p 2946-2953 -0.319 94.0 -0.039 45.0 noncon
hsa-miR-1587 1366-1373 -0.312 96.0 -0.038 59.0 noncon
hsa-miR-5590-5p 2944-2951 -0.308 97.0 -0.038 59.0 noncon
hsa-miR-6791-3p 2588-2595 -0.306 97.0 -0.038 72.0 noncon
hsa-miR-4656 1365-1372 -0.306 97.0 -0.038 48.0 noncon
hsa-miR-4800-5p 2276-2282 -0.302 93.0 -0.037 52.0 noncon
hsa-miR-671-5p 52-59 -0.299 95.0 -0.299 96.0 noncon
hsa-miR-6879-5p 385-392 -0.299 96.0 -0.05 66.0 noncon
hsa-miR-6735-5p 385-392 -0.299 96.0 -0.05 66.0 noncon
hsa-miR-4436b-3p 385-392 -0.299 96.0 -0.05 66.0 noncon
hsa-miR-3064-3p 171-177 -0.298 94.0 -0.298 95.0 noncon
hsa-miR-6785-5p 549-555 -0.293 96.0 -0.049 68.0 noncon
hsa-miR-298 414-421 -0.291 97.0 -0.048 70.0 noncon
hsa-miR-1255a 538-545 -0.287 94.0 -0.048 59.0 noncon
hsa-miR-6858-3p 22-28 -0.286 97.0 -0.286 98.0 noncon
hsa-miR-6829-3p 2588-2595 -0.282 97.0 -0.035 70.0 noncon
hsa-miR-604 1548-1555 -0.282 97.0 -0.035 56.0 noncon
hsa-miR-1270 426-433 -0.282 97.0 -0.047 74.0 noncon
hsa-miR-92a-1-5p 1354-1361 -0.282 90.0 -0.035 46.0 noncon
hsa-miR-1255b-5p 538-545 -0.282 94.0 -0.047 60.0 noncon
hsa-miR-155-3p 3075-3081 -0.279 92.0 -0.034 51.0 noncon
hsa-miR-620 426-433 -0.279 97.0 -0.047 73.0 noncon
hsa-miR-4999-3p 39-45 -0.278 93.0 -0.278 96.0 noncon
hsa-miR-874-3p 3020-3026 -0.275 95.0 -0.034 62.0 noncon
hsa-miR-6743-5p 2828-2835 -0.275 94.0 -0.034 40.0 noncon
hsa-miR-6883-5p 355-362 -0.269 95.0 -0.045 65.0 noncon
hsa-miR-6871-3p 18-24 -0.265 95.0 -0.265 96.0 noncon
hsa-miR-483-3p.2 2623-2630 -0.263 96.0 -0.033 72.0 noncon
hsa-miR-149-3p 355-362 -0.261 94.0 -0.044 62.0 noncon
hsa-miR-6836-3p 2589-2596 -0.261 94.0 -0.032 68.0 noncon
hsa-miR-3925-5p 633-640 -0.258 98.0 -0.043 86.0 noncon
hsa-miR-614 229-235 -0.252 90.0 -0.042 41.0 noncon
hsa-miR-4728-5p 355-362 -0.251 93.0 -0.042 62.0 noncon
hsa-miR-6785-5p 355-362 -0.251 93.0 -0.042 66.0 noncon
hsa-miR-195-5p 582-588 -0.25 92.0 -0.042 63.0 noncon