GlyClass

GlycoEnzDB
Comprehensive glycoEnzyme database and dynamic pathway generation

Description: The GlycoEnzDB is a manually curated glycoEnzyme database, primarily focused on humans. It covers 390 enzymes across 28 pathway maps. Facilities are also available to create custom glycosylation reaction pathways using experimental data in SBML format and for pathway simulation
Contacts: Sriram Neelamegham (neel@buffalo.edu), Yusen Zhou (yusenzho@buffalo.edu) or Ted Groth (tgroth@buffalo.edu).

GlycoEnzDB
Create glycosylation pathway
Custom database generation
Instructions/Reference
 
Pathway Map
HEXB



General Information
Gene Symbol:   
HEXB
Protein name:   
Beta-hexosaminidase subunit beta
Enzyme Class:   
Glycosidase
CAZy Family:   
GH20
E.C. No:   
3.2.1.52
Organs:   
Reaction (SNFG):   
→
Reaction (IUBMB):   
No specified reaction.
About

• Beta hexosaminidase A and B interact with one another to degrade the GM2 ganglioside in lysosomes by removing GalNAc. These enzymes are important for regulating glycolipid homeostasis in the central nervous system.
• Defects in HEXA result in Tay Sachs disease, a condition which results in the buildup of lipids in the brain. This results in seizures, paralysis, and eventually death at an early age.
• HAEXA/B also facilitate glycoproteins (N- and O-) and keratin sulfate degradation

Related Pathway
KEGG ORTHOLOGY:   
KEGG Pathway:   
No Record 
Reactome:   
R-HSA-1660662
Related Database
Organism:   
Homo sapiens
Uniprot:   
P07686 
NCBI GeneID:   
3074
GlyMap GeneID:   
3074
DNA aRefSeq:   
NM_000521.3 
Protein RefSeq:   
NP_000512.1 
BRENDA:   
Kinetic info.
OMIM number:   
606873
GeneCards database:   
HEXB
Enzyme Specificity
Glycan Type:  
     GSL
Compartment:  
     
Substrate:  
     GalNAc(b1-?)
Product:  
     
Constraints:  
     None
Simulation Parameters
Mechaelis-Menten parameters (suggested)
VM:       (pmol/cell • hr)       KM:       (pM)
Relative conc of enzymes in compartment.(sum will be normalized to 1)
ER Cis- Medial- Trans- TGN










Expression Data in Tissue {ref. 5} Source from NCBI gene database
adrenal appendix bone marrow brain colon duodenum endometrium esophagus fat gall bladder heart kidney liver lung lymph node ovary pancreas placenta prostate salivary gland skin small intestine spleen stomach testis thyroid urinary bladder 140 120 100 80 60 40 20 0 RPKM
Expression Data in Cells {ref. 13} Source from Cancer Cell Line Encyclopedia database
Kidney Lung Breast Prostate Large intestine Ovary HL60 haematopoietic and lymphoid tissue Skin Central nervous system 786O A498 ACHN CAKI1 UO31 A549 EKVX HOP62 HOP92 NCIH226 NCIH23 NCIH322 NCIH460 NCIH522 SHP77 BT549 HS578T MCF7 MDAMB231 MDAMB468 T47D DU145 PC3 HCT116 HCT15 HT29 KM12 SW620 OVCAR4 OVCAR8 IGROV1 SKOV3 HL60 K562 RPMI8226 LOXIMVI MALME3M RPMI7951 SKMEL28 SKMEL5 UACC257 UACC62 SF268 SF295 SF539 SNB75 500 400 300 200 100 0 Transcripts per million (TPM)
Transcription factor {ref. 10}
Transcriptional start site (TSS) 0 200 -2000 -1800 -1600 -1400 -1200 -1000 -800 -600 -400 -200
ENST00000261416 ENST00000511181 JARID2 MYCN TBP TFAP2C GLIS1 ZFX UBTF YY1 PAX5 EGR3 MYCN TAL1
miRNA-GlycoEnzyme Interactome {ref. 9}
miRNA Name Pos. Context Score Frac. Wt. Context Frac. Conserved Quality
hsa-miR-137 407-414 -0.751 99.0 -0.001 22.0 noncon
hsa-miR-508-3p 36-43 -0.686 99.0 -0.686 99.0 noncon
hsa-miR-95-5p 101-108 -0.575 99.0 -0.575 99.0 noncon
hsa-miR-30a-3p 426-433 -0.57 99.0 0.0 0.0 noncon
hsa-miR-30d-3p 426-433 -0.57 99.0 0.0 0.0 noncon
hsa-miR-30e-3p 426-433 -0.57 99.0 0.0 0.0 noncon
hsa-miR-3614-3p 215-222 -0.569 99.0 0.0 35.0 noncon
hsa-miR-4755-3p 145-152 -0.52 99.0 0.0 26.0 noncon
hsa-miR-212-3p 78-84 -0.494 99.0 -0.494 99.0 noncon
hsa-miR-132-3p 78-84 -0.483 99.0 -0.483 99.0 noncon
hsa-miR-562 219-226 -0.458 99.0 0.0 34.0 noncon
hsa-miR-100-3p 167-174 -0.448 99.0 0.0 35.0 noncon
hsa-miR-4662a-3p 125-132 -0.421 99.0 0.0 37.0 noncon
hsa-miR-505-5p 316-323 -0.417 99.0 0.0 20.0 noncon
hsa-miR-7108-5p 18-24 -0.396 99.0 -0.396 99.0 noncon
hsa-miR-4670-3p 233-240 -0.383 99.0 0.0 19.0 noncon
hsa-miR-1178-5p 118-124 -0.365 97.0 0.0 11.0 noncon
hsa-miR-4804-5p 393-399 -0.356 90.0 0.0 6.0 noncon
hsa-miR-4445-5p 37-43 -0.348 97.0 -0.348 98.0 noncon
hsa-miR-7153-5p 360-366 -0.344 98.0 0.0 21.0 noncon
hsa-miR-4676-3p 378-385 -0.339 98.0 0.0 29.0 noncon
hsa-miR-3919 362-369 -0.334 99.0 0.0 35.0 noncon
hsa-miR-146b-5p 360-366 -0.333 98.0 0.0 21.0 noncon
hsa-miR-15b-3p 253-260 -0.331 98.0 0.0 29.0 noncon
hsa-miR-892c-3p 378-385 -0.331 98.0 0.0 28.0 noncon
hsa-miR-452-5p 378-385 -0.328 98.0 0.0 26.0 noncon
hsa-miR-28-3p 356-362 -0.327 98.0 0.0 22.0 noncon
hsa-miR-3613-3p 83-90 -0.326 99.0 -0.326 99.0 noncon
hsa-miR-218-5p 283-289 -0.322 96.0 0.0 28.0 noncon
hsa-miR-146a-5p 360-366 -0.322 98.0 0.0 21.0 noncon
hsa-miR-577 353-360 -0.318 99.0 0.0 28.0 noncon
hsa-miR-889-3p 71-77 -0.314 99.0 -0.314 99.0 noncon
hsa-miR-891a-3p 324-330 -0.314 97.0 0.0 28.0 noncon
hsa-miR-5590-3p 44-50 -0.312 99.0 -0.312 99.0 noncon
hsa-miR-4757-5p 141-147 -0.307 96.0 0.0 22.0 noncon
hsa-miR-181b-5p 367-374 -0.306 97.0 0.0 22.0 noncon
hsa-miR-181a-5p 367-374 -0.306 97.0 0.0 22.0 noncon
hsa-miR-4666a-5p 59-65 -0.305 97.0 -0.305 98.0 noncon
hsa-miR-6873-5p 121-127 -0.304 97.0 0.0 14.0 noncon
hsa-miR-548av-5p 383-390 -0.302 99.0 0.0 51.0 noncon
hsa-miR-548k 383-390 -0.302 99.0 0.0 50.0 noncon
hsa-miR-181c-5p 367-374 -0.298 97.0 0.0 23.0 noncon
hsa-miR-1278 381-387 -0.295 97.0 0.0 21.0 noncon
hsa-miR-3925-5p 361-367 -0.291 99.0 0.0 52.0 noncon
hsa-miR-181d-5p 367-374 -0.282 96.0 0.0 22.0 noncon
hsa-miR-3677-5p 326-332 -0.276 95.0 0.0 34.0 noncon
hsa-miR-708-3p 357-363 -0.274 97.0 0.0 16.0 noncon
hsa-miR-4782-3p 37-43 -0.272 93.0 -0.272 95.0 noncon
hsa-miR-4252 265-271 -0.272 97.0 0.0 26.0 noncon
hsa-miR-4705 106-112 -0.271 96.0 0.0 34.0 noncon
hsa-miR-5187-3p 255-261 -0.271 98.0 0.0 42.0 noncon
hsa-miR-663b 18-24 -0.271 95.0 -0.271 96.0 noncon
hsa-miR-142-5p 44-50 -0.264 98.0 -0.264 99.0 noncon
hsa-miR-6766-3p 37-43 -0.26 93.0 -0.26 95.0 noncon
hsa-miR-219a-5p 37-43 -0.26 93.0 -0.26 95.0 noncon
hsa-miR-539-5p 289-296 -0.256 99.0 0.0 47.0 noncon
hsa-miR-6744-3p 141-147 -0.255 97.0 0.0 34.0 noncon
hsa-miR-5006-5p 146-152 -0.25 93.0 0.0 21.0 noncon