GlyClass

GlycoEnzDB
Comprehensive glycoEnzyme database and dynamic pathway generation

Description: The GlycoEnzDB is a manually curated glycoEnzyme database, primarily focused on humans. It covers 390 enzymes across 28 pathway maps. Facilities are also available to create custom glycosylation reaction pathways using experimental data in SBML format and for pathway simulation
Contacts: Sriram Neelamegham (neel@buffalo.edu), Yusen Zhou (yusenzho@buffalo.edu) or Ted Groth (tgroth@buffalo.edu).

GlycoEnzDB
Create glycosylation pathway
Custom database generation
Instructions/Reference
 
Pathway Map
ST8SIA1



General Information
Gene Symbol:   
ST8SIA1
Protein name:   
Alpha-N-acetylneuraminide alpha-2,8-sialyltransferase
Enzyme Class:   
Glycosyltransferase
CAZy Family:   
GT29
E.C. No:   
2.4.99.8
Organs:   
Reaction (SNFG):   
+
→
+ CMP
+
→
+ CMP
Reaction (IUBMB):   
No specified reaction.
About

• Key enzyme for the biosynthesis of b-series gangliosides on glycolipids, thus called GD3 synthase. ST8Sia-I uses GM3 (Neu5Acα2-3Galβ1-4Glcβ1-O-Cer) to form GD3 (Neu5Acα2-8Neu5Acα2-3Galβ1-4Glcβ1-O-Cer) (b-series). This can then form GT3 (c-series gangliosides). ST8Sia I and b- and c-series gangliosides are implicated in oncogenesis by mediating cell proliferation, migration, tumor growth, and angiogenesis. GD3 is weakly expressed in most normal cells but upregulated in cancer.
• Expressed in various organs, particularly the brain, and overexpressed in neuroectoderm-derived malignant cells such as melanoma, glioblastoma, neuroblastoma, and estrogen receptor-negative breast cancer.
• ST8SIA1 knockout mice completely lack b- and c-series gangliosides. However, they show almost complete nervous tissue morphology and no apparent abnormal behavior. But they show reduced reneration of nerve, suggesting a role for the enzyme in repair rather than normal differentiation.
• ST8SIA1 displayed higher expression among ER-negative breast cancer tumors and this is assocaiated with poor pathohistological grading.

Related Pathway
KEGG ORTHOLOGY:   
K03371
KEGG Pathway:   
ko00601  ko00603  ko00604  ko01100 
Reactome:   
R-HSA-4085001
Related Database
Organism:   
Homo sapiens
Uniprot:   
Q92185 
NCBI GeneID:   
6489
GlyMap GeneID:   
6489
DNA aRefSeq:   
NM_001304450.1 
Protein RefSeq:   
NP_001291379.1 
BRENDA:   
Kinetic info.
OMIM number:   
601123
GeneCards database:   
ST8SIA1
Enzyme Specificity
Glycan Type:  
     GSL
Compartment:  
     
Substrate:  
     NeuAc(a2-3)Gal(b1-?) or NeuAc(a2-8)NeuAc(a2-3)Gal(b1-?)
Product:  
     NeuAc(a2-8)NeuAc(a2-3)Gal(b1-?) or NeuAc(a2-8)NeuAc(a2-8)NeuAc(a2-3)Gal(b1-?)
Simulation Parameters
Mechaelis-Menten parameters (suggested)
VM:       (pmol/cell • hr)       KM:       (pM)
Relative conc of enzymes in compartment.(sum will be normalized to 1)
ER Cis- Medial- Trans- TGN










Expression Data in Tissue {ref. 5} Source from NCBI gene database
adrenal appendix bone marrow brain colon duodenum endometrium esophagus fat gall bladder heart kidney liver lung lymph node ovary pancreas placenta prostate salivary gland skin small intestine spleen stomach testis thyroid urinary bladder 4 2 0 RPKM
Expression Data in Cells {ref. 13} Source from Cancer Cell Line Encyclopedia database
Kidney Lung Breast Prostate Large intestine Ovary HL60 haematopoietic and lymphoid tissue Skin Central nervous system 786O A498 ACHN CAKI1 UO31 A549 EKVX HOP62 HOP92 NCIH226 NCIH23 NCIH322 NCIH460 NCIH522 SHP77 BT549 HS578T MCF7 MDAMB231 MDAMB468 T47D DU145 PC3 HCT116 HCT15 HT29 KM12 SW620 OVCAR4 OVCAR8 IGROV1 SKOV3 HL60 K562 RPMI8226 LOXIMVI MALME3M RPMI7951 SKMEL28 SKMEL5 UACC257 UACC62 SF268 SF295 SF539 SNB75 100 80 60 40 20 0 Transcripts per million (TPM)
Transcription factor {ref. 10}
No high degree of confidence data
miRNA-GlycoEnzyme Interactome {ref. 9}
miRNA Name Pos. Context Score Frac. Wt. Context Frac. Conserved Quality
hsa-miR-644a 99-106 -0.624 99.0 -0.622 99.0 noncon
hsa-miR-324-5p 204-211 -0.609 99.0 -0.608 99.0 noncon
hsa-miR-760 95-102 -0.552 99.0 -0.551 99.0 noncon
hsa-miR-4509 515-522 -0.548 99.0 -0.547 99.0 noncon
hsa-miR-1256 870-877 -0.547 99.0 -0.546 99.0 noncon
hsa-miR-106a-3p 921-928 -0.532 99.0 -0.531 99.0 noncon
hsa-miR-125b-2-3p 559-566 -0.516 99.0 -0.515 99.0 noncon
hsa-miR-630 326-333 -0.513 99.0 -0.512 99.0 noncon
hsa-miR-6762-3p 31-38 -0.499 99.0 -0.498 99.0 noncon
hsa-miR-6892-3p 23-30 -0.499 99.0 -0.498 99.0 noncon
hsa-miR-3156-5p 120-127 -0.497 99.0 -0.496 99.0 noncon
hsa-miR-8073 70-77 -0.491 99.0 -0.49 99.0 noncon
hsa-miR-6765-3p 154-161 -0.488 99.0 -0.487 99.0 noncon
hsa-miR-3619-5p 380-387 -0.486 99.0 -0.485 99.0 noncon
hsa-miR-221-5p 70-77 -0.475 99.0 -0.474 99.0 noncon
hsa-miR-214-3p 380-387 -0.472 99.0 -0.471 99.0 noncon
hsa-miR-513b-5p 560-567 -0.463 99.0 -0.462 99.0 noncon
hsa-miR-5197-3p 966-973 -0.461 99.0 -0.46 99.0 noncon
hsa-miR-647 32-39 -0.457 99.0 -0.456 99.0 noncon
hsa-miR-501-5p 778-785 -0.454 99.0 -0.453 99.0 noncon
hsa-miR-548m 513-520 -0.452 99.0 -0.451 99.0 noncon
hsa-miR-4261 224-231 -0.446 99.0 -0.445 99.0 noncon
hsa-miR-8485 443-450 -0.445 99.0 -0.444 99.0 noncon
hsa-miR-202-5p 723-730 -0.439 99.0 -0.438 99.0 noncon
hsa-miR-4291 381-388 -0.437 99.0 -0.436 99.0 noncon
hsa-miR-4275 813-820 -0.434 99.0 -0.433 99.0 noncon
hsa-miR-890 614-621 -0.431 99.0 -0.43 99.0 noncon
hsa-miR-660-3p 73-80 -0.427 99.0 -0.426 99.0 noncon
hsa-miR-761 380-387 -0.426 99.0 -0.425 99.0 noncon
hsa-miR-4452 908-915 -0.425 99.0 -0.424 99.0 noncon
hsa-miR-6740-3p 133-140 -0.422 98.0 -0.421 98.0 noncon
hsa-miR-8068 826-833 -0.416 98.0 -0.415 98.0 noncon
hsa-miR-4528 902-909 -0.404 99.0 -0.403 99.0 noncon
hsa-miR-8485 879-886 -0.397 99.0 -0.396 99.0 noncon
hsa-miR-600 978-985 -0.386 99.0 -0.385 99.0 noncon
hsa-miR-197-3p 1078-1084 -0.376 99.0 -0.375 99.0 noncon
hsa-miR-583 740-747 -0.375 99.0 -0.374 99.0 noncon
hsa-miR-1185-5p 487-493 -0.371 98.0 -0.37 98.0 noncon
hsa-miR-548t-5p 1059-1066 -0.371 99.0 -0.37 99.0 noncon
hsa-miR-548az-5p 1059-1066 -0.371 99.0 -0.37 99.0 noncon
hsa-miR-548l 633-640 -0.368 99.0 -0.367 99.0 noncon
hsa-miR-642a-5p 24-31 -0.361 98.0 -0.36 99.0 noncon
hsa-miR-3679-5p 487-493 -0.359 98.0 -0.358 98.0 noncon
hsa-miR-15a-5p 652-658 -0.358 97.0 -0.357 98.0 noncon
hsa-miR-4659b-3p 369-376 -0.351 98.0 -0.35 98.0 noncon
hsa-miR-4659a-3p 369-376 -0.351 98.0 -0.35 98.0 noncon
hsa-miR-4690-5p 379-385 -0.349 99.0 -0.348 99.0 noncon
hsa-miR-16-5p 652-658 -0.347 97.0 -0.346 98.0 noncon
hsa-miR-195-5p 652-658 -0.347 97.0 -0.346 98.0 noncon
hsa-miR-3188 737-743 -0.337 98.0 -0.336 98.0 noncon
hsa-miR-539-5p 1053-1060 -0.336 99.0 -0.335 99.0 noncon
hsa-miR-15b-5p 652-658 -0.336 97.0 -0.335 98.0 noncon
hsa-miR-424-5p 652-658 -0.333 97.0 -0.332 97.0 noncon
hsa-miR-4789-5p 882-889 -0.326 99.0 -0.325 99.0 noncon
hsa-miR-5580-3p 698-705 -0.322 99.0 -0.321 99.0 noncon
hsa-miR-1267 912-918 -0.322 98.0 -0.321 98.0 noncon
hsa-miR-6851-3p 174-180 -0.314 96.0 -0.313 97.0 noncon
hsa-miR-3941 444-450 -0.313 98.0 -0.312 99.0 noncon
hsa-miR-593-5p 68-74 -0.311 93.0 -0.31 95.0 noncon
hsa-miR-497-5p 652-658 -0.311 96.0 -0.31 97.0 noncon
hsa-miR-107 651-657 -0.31 96.0 -0.309 97.0 noncon
hsa-miR-103a-3p 651-657 -0.31 96.0 -0.309 97.0 noncon
hsa-miR-4436b-5p 80-87 -0.309 98.0 -0.308 98.0 noncon
hsa-miR-4648 185-191 -0.308 96.0 -0.307 97.0 noncon
hsa-miR-3182 131-138 -0.306 99.0 -0.305 99.0 noncon
hsa-miR-4770 962-968 -0.305 98.0 -0.304 98.0 noncon
hsa-miR-6838-5p 652-658 -0.304 95.0 -0.303 97.0 noncon
hsa-miR-144-3p 1026-1032 -0.299 97.0 -0.298 98.0 noncon
hsa-miR-583 490-496 -0.299 99.0 -0.298 99.0 noncon
hsa-miR-143-3p 962-968 -0.294 97.0 -0.293 98.0 noncon
hsa-miR-4665-5p 271-277 -0.292 92.0 -0.291 94.0 noncon
hsa-miR-4531 107-113 -0.291 97.0 -0.29 98.0 noncon
hsa-miR-4727-3p 187-193 -0.29 92.0 -0.289 94.0 noncon
hsa-miR-513b-3p 818-824 -0.29 98.0 -0.289 98.0 noncon
hsa-miR-31-5p 196-202 -0.283 94.0 -0.282 96.0 noncon
hsa-miR-3941 880-886 -0.281 98.0 -0.28 98.0 noncon
hsa-miR-5587-5p 158-164 -0.281 95.0 -0.28 97.0 noncon
hsa-miR-4291 654-660 -0.28 96.0 -0.279 97.0 noncon
hsa-miR-4520-2-3p 612-618 -0.279 91.0 -0.278 94.0 noncon
hsa-miR-1275 271-277 -0.277 95.0 -0.276 96.0 noncon
hsa-miR-3529-3p 707-714 -0.277 98.0 -0.276 99.0 noncon
hsa-miR-101-3p.1 755-761 -0.276 96.0 -0.275 97.0 noncon
hsa-miR-4474-3p 98-104 -0.273 94.0 -0.272 96.0 noncon
hsa-miR-96-3p 258-264 -0.272 96.0 -0.271 97.0 noncon
hsa-miR-3119 375-381 -0.272 95.0 -0.271 96.0 noncon
hsa-miR-640 861-867 -0.267 95.0 -0.266 96.0 noncon
hsa-miR-4645-3p 756-762 -0.266 94.0 -0.265 96.0 noncon
hsa-miR-217 950-956 -0.266 97.0 -0.265 98.0 noncon
hsa-miR-9-3p 573-579 -0.266 96.0 -0.265 97.0 noncon
hsa-miR-6822-3p 96-102 -0.266 95.0 -0.265 96.0 noncon
hsa-miR-3119 735-741 -0.266 95.0 -0.265 96.0 noncon
hsa-miR-4289 163-170 -0.265 98.0 -0.264 98.0 noncon
hsa-miR-146a-3p 144-150 -0.262 96.0 -0.261 98.0 noncon
hsa-miR-6792-3p 75-81 -0.254 93.0 -0.253 95.0 noncon
hsa-miR-151a-3p 953-959 -0.253 92.0 -0.252 95.0 noncon
hsa-miR-873-3p 419-425 -0.253 97.0 -0.252 98.0 noncon
hsa-miR-604 32-38 -0.253 95.0 -0.252 96.0 noncon
hsa-miR-6768-3p 177-183 -0.251 96.0 -0.25 97.0 noncon