Glycogene CRISPR Library Viewer

Usage Instructions:

If you use this resource, please cite: Y. Zhu, T. Groth, A. Kelkar, Y. Zhou, S. Neelamegham “A GlycoGene CRISPR-Cas9 lentiviral library to study lectin binding and human glycan biosynthesis pathways”, Glycobiology, 2020 (in press) [.pdf].
All plasmids will be available from Addgene. If you need reagents urgently, please write to

This app allows users to browse sgRNA and associated data collected using the Glycogene CRISPR sgRNA library. To use:

  • "Select Glycogene:" using drop-down menu
  • “Select Gene Transcript:” as many genes have more than one protein-coding transcript variant.

Information about sgRNA targeting the selected genes, location on transcript and experimental data regarding sgRNA enrichment/depletion will appear in table format.

To determine glycogene transcripts appearing in selected, common cancer cell lines:

  • Select tissue type
  • Select cell line

Data shown is rendered as heat maps using RSEM (RNA-seq by Expectation Maximization) collected from the Cancer Cell Line Encyclopedia (CCLE) (ref 3). An RSEM value of 1 corresponds to the top 20% of RSEM values in the CCLE for the selected NCI60 cell lines. Thus, RSEM values > 1 are considered to be highly expressed.


  1. Y. Zhu, et al. Glycobiology, 2020 (in press) [link]
  2. Li, W. et al. Genome Biol 15, 554 (2014).
  3. [link]
  4. Barretina, J. et al. Nature 483, 603–607 (2012).
  5. [link]

sgRNA Sequence and Location Table

Gene Rank & False Discover Rate

sgRNA Log2 Fold Change Upon Lectin Enrichment

Selected CCLE Transcript RSEM Data