Virtual Glycome

ST3Gal-4 controls L-, E- and P-selectin ligand biosynthesis on human myeloid cells

Ablation of human ST3Gal-4 using CRISPR-Cas9 prevents sialyl Lewis-X biosynthesis on human leukocyte O- and N-linked glycans. It also prevents cell adhesion to all types of adhesion molecules belonging to the selectin family. [Read more]

Differences in the post-translational machinery in humans versus mice?

Mouse, but not human, neutrophil adhesion to IL-1β stimulated vascular endothelial cell is abrogated upon pronase treatment of the leukocytes. Thus, glycolipids may uniquely contribute to human myeloid cell adhesion during inflammation. [Read more]

Glycoengineering stem cells to target cardiac ischemia-reperfusion injury in the pig heart

The low homing and engraftment of stem cells to damaged tissue continues to be a problem for regenerative medicine approaches aimed at resolving cardiology problems. Glycoengineering stem cells with selectin-ligands provides an avenue to address this challenge. [Read more]

Integration of systems glycobiology with bioinformatics toolboxes, glycoinformatics resources, and glycoproteomics data

In silico modeling of glycosylation pathways requires standardized modeling practices that can be shared among laboratories, and the integration of glycoinformatics resources with the computational models. [Read more]

Welcome to the Virtual Glycome

The glycome constitutes the entire complement of free carbohydrates and glycoconjugates expressed in cells or tissue. ‘Systems Glycobiology’ is an emerging discipline that aims to quantitatively describe and analyze the glycome.

This website is focused on presenting selected computational tools and experimental resources that can be used to better understand the processes regulating cellular glycosylation at multiple levels.

The pictoral representation below describes the different computational tools available at this resource, and their relationship to the central dogma that is extended in the Golgi to form the glycome on individual cells.

ST3Gal-4 controls L-, E- and P-selectin ligand biosynthesis on human myeloid cells

Differences in the post-translational machinery in humans versus mice?

Glycoengineering stem cells to target cardiac ischemia-reperfusion injury in the pig heart

Integration of systems glycobiology with bioinformatics toolboxes, glycoinformatics resources, and glycoproteomics data

Welcome to the Virtual Glycome

Information Flow